mRNA levels of the linker histone variant, H1o, in mitotically active human diploid fibroblasts as a function of the phases of the cell cycle and cumulative population doublings

2001 ◽  
Vol 121 (1-3) ◽  
pp. 101-112 ◽  
Author(s):  
Dimitra S Tsapali ◽  
Kalliope E Sekeri-Pataryas ◽  
Anastasia Prombona ◽  
Thomae G Sourlingas
Keyword(s):  
Cell Cycle ◽  
Linker Histone ◽  
Histone Variant ◽  
Mrna Levels ◽  
Diploid Fibroblasts ◽  
Human Diploid Fibroblasts ◽  
Population Doublings

scholarly journals Tocotrienol-Rich Fraction Prevents Cell Cycle Arrest and Elongates Telomere Length in Senescent Human Diploid Fibroblasts

2011 ◽  
Vol 2011 ◽  
pp. 1-11 ◽  
Author(s):  
Suzana Makpol ◽  
Lina Wati Durani ◽  
Kien Hui Chua ◽  
Yasmin Anum Mohd Yusof ◽  
Wan Zurinah Wan Ngah
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Telomere Length ◽  
Telomerase Activity ◽  
S Phase ◽  
Diploid Fibroblasts ◽  
Human Diploid Fibroblasts ◽  
Cycle Arrest ◽  
Tocotrienol Rich Fraction ◽  
Damaged Dna

This study determined the molecular mechanisms of tocotrienol-rich fraction (TRF) in preventing cellular senescence of human diploid fibroblasts (HDFs). Primary culture of HDFs at various passages were incubated with 0.5 mg/mL TRF for 24 h. Telomere shortening with decreased telomerase activity was observed in senescent HDFs while the levels of damaged DNA and number of cells in G0/G1phase were increased and S phase cells were decreased. Incubation with TRF reversed the morphology of senescent HDFs to resemble that of young cells with decreased activity of SA-β-gal, damaged DNA, and cells in G0/G1phase while cells in the S phase were increased. Elongated telomere length and restoration of telomerase activity were observed in TRF-treated senescent HDFs. These findings confirmed the ability of tocotrienol-rich fraction in preventing HDFs cellular ageing by restoring telomere length and telomerase activity, reducing damaged DNA, and reversing cell cycle arrest associated with senescence.

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