[39] Assay of menaquinones in bacterial cultures, stool samples, and intestinal contents

1997 ◽  
pp. 457-466 ◽  
Author(s):  
John M. Conly
Keyword(s):  
Bacterial Cultures ◽  
Intestinal Contents ◽  
Stool Samples

scholarly journals Dipstick Test for Rapid Diagnosis of Shigella dysenteriae 1 in Bacterial Cultures and Its Potential Use on Stool Samples

PLoS ONE ◽  
2011 ◽  
Vol 6 (10) ◽  
pp. e24830 ◽  
Author(s):  
Neelam Taneja ◽  
Faridabano Nato ◽  
Sylvie Dartevelle ◽  
Jean Marie Sire ◽  
Benoit Garin ◽  
...  
Keyword(s):  
Rapid Diagnosis ◽  
Shigella Dysenteriae ◽  
Dipstick Test ◽  
Bacterial Cultures ◽  
Stool Samples ◽  
Potential Use

scholarly journals Microbial landscape of intestinal contents and mucus in frequently ill children

2016 ◽  
Vol 97 (6) ◽  
pp. 850-853 ◽  
Author(s):  
M K Kerimova
Keyword(s):  
Staphylococcus Aureus ◽  
Chlamydia Pneumoniae ◽  
Throat Swab ◽  
Immunoglobulin A ◽  
Healthy Children ◽  
Upper Respiratory Tract ◽  
Bacteriological Study ◽  
Intestinal Contents ◽  
Mycoplasma Pneumonia ◽  
Stool Samples

Aim. To study the state of the microbial landscape of intestinal contents and mucus in frequently ill children and practically healthy children.Methods. 340 frequently ill children aged 6 months to 6 years and 125 practically healthy children (comparison group) were examined. Along with routine clinical and immunological studies bacteriological study of stool samples and throat swab was performed and polymerase chain reaction was applied to the swab.Results. According to the results of bacteriological study of throat swab 195 (57.4%) children reported Staphylococcus aureus, 102 (30.0%) children - Staphylococcus epidermidis, 85 (25.0%) children - Streptococcus pneumoniae, 64 (18.8%) children - Haemophilus influenzae, 64 (18.8%) children - Klebsiella pneumoniae, 170 (50.0%) children - Candida albicans. PCR revealed Chlamydia pneumoniae in 53 (15.6%) children, Chlamydia trachomatis in 33 (9.7%) children, Mycoplasma pneumonia in 54 (15.9%) children, and Cytomegalovirus in 18 (5.3%) patients. Among frequently ill children in 67.6% of cases dysbiosis was observed with reduced number of bifidobacteria and lactobacilli and increase of opportunistic microorganisms, predominantly Staphylococcus aureus.Conclusion. The microbiota of upper respiratory tract in frequently ill children in most cases contains Staphylococcus aureus; reduced number of bifidobacteria and lactobacilli and increase of opportunistic microorganisms were noted; correlation between severity of dysbiosis and immunoglobulin A level in blood was revealed.

scholarly journals High purity galacto-oligosaccharides enhance specific Bifidobacterium species and their metabolic activity in the mouse gut microbiome

2016 ◽  
Vol 7 (2) ◽  
pp. 247-264 ◽  
Author(s):  
A. Monteagudo-Mera ◽  
J.C. Arthur ◽  
C. Jobin ◽  
T. Keku ◽  
J.M. Bruno-Barcena ◽  
...  
Keyword(s):  
Intestinal Bacteria ◽  
Intestinal Microbiome ◽  
Gastrointestinal Microbiota ◽  
Necrosis Factor Alpha ◽  
Bifidobacterium Adolescentis ◽  
Bifidobacterium Lactis ◽  
Control Female ◽  
Intestinal Contents ◽  
Stool Samples ◽  
Factor Alpha

Prebiotics are selectively fermented ingredients that result in specific changes in the composition and/or activity of the gastrointestinal microbiota, thus conferring benefit(s) upon the host health. The aim of this study was to evaluate the influence of a β(1-4)galacto-oligosaccharides (GOS) formulation consisting of 90% pure GOS (GOS90), on the composition and activity of the mouse gut microbiota. Germ-free mice were colonised with microbiota from four pathogen-free wt 129 mice donors (SPF), and stools were collected during a feeding trial in which GOS90 was delivered orally for 14 days. Pyrosequencing of 16S rDNA amplicons showed that Bifidobacterium and specific Lactobacillus, Bacteroides and Clostridiales were more prevalent in GOS90-fed mice after 14 days, although the prebiotic impact on Bifidobacterium varied among individual mice. Prebiotic feeding also resulted in decreased abundance of Bacteroidales, Helicobacter and Clostridium. High-throughput quantitative PCR showed an increased abundance of Bifidobacterium adolescentis, Bifidobacterium pseudocatenulatum, Bifidobacterium lactis and Bifidobacterium gallicum in the prebiotic-fed mice. Control female mice showed a higher diversity (phylogenetic diversity (PD) = 15.1±3.4 in stools and PD = 13.0±0.6 in intestinal contents) than control males (PD = 7.8±1.6 in stool samples and PD = 9.5±1.0 in intestinal contents). GOS90 did not modify inflammatory biomarkers (interleukin (IL)-6, IL-12, IL-1β, interferon gamma and tumour necrosis factor alpha). Decreased butyrate, acetate and lactate concentrations in stools of prebiotic fed mice suggested an increase in colonic absorption and reduced excretion. Overall, our results demonstrate that GOS90 is capable of modulating the intestinal microbiome resulting in expansion of the probiome (autochtonous commensal intestinal bacteria considered to have a beneficial influence on health).

Bacteriophages Associated with Turkey Coecums in Bluecomb-Infected and Healthy Birds

1969 ◽  
Vol 27 ◽  
pp. 226-227
Author(s):  
A. E. Ritchie
Keyword(s):  
Host Cell ◽  
Causal Relationship ◽  
Cell Cultures ◽  
Cell Interaction ◽  
Etiologic Agent ◽  
Viral Origin ◽  
Intestinal Contents ◽  
Host Cell Interaction

The cause of bluecomb disease in turkeys is unknown. Filtration of infective intestinal contents suggests a viral origin. To date, it has not been possible to isolate the etiologic agent in various cell cultures. The purpose of this work was to characterize as many virus-like entities as were recognizable in intestines of both healthy and bluecomb-infected turkeys. By a comparison of the viral populations it was hoped that some insight might be gained into the cause of this disease. Studies of turkey hemorraghic enteritis by Gross and Moore (Avian Dis. 11: 296-307, 1967) have suggested that a bacteriophage-host cell interaction may bear some causal relationship to that disease.

C. difficile, Other Pathogens Often in ED Stool Samples

2006 ◽  
Vol 40 (11) ◽  
pp. 17
Author(s):  
JOHN R. BELL
Keyword(s):  
Stool Samples

Rapid detection of Rotavirus antigen in stool sample of acute diarrheic children

2012 ◽  
Vol 6 (1) ◽  
pp. 11-13
Author(s):  
Sushmita Roy ◽  
S.M. Shamsuzzaman ◽  
K.Z. Mamun
Keyword(s):  
Stool Sample ◽  
Rapid Detection ◽  
Cross Sectional Study ◽  
Rapid Diagnosis ◽  
Age Group ◽  
College Hospital ◽  
Cross Sectional ◽  
Rotavirus Infection ◽  
Medical College ◽  
Stool Samples

Rotavirus is one of the leading causes of pediatric diarrhea globally. Accurate and rapid diagnosis of Rotavirus diarrhea should reduce unnecessary use of antibiotics and ultimately reduce drug resistance. Study was designed for rapid diagnosis of Rotavirus antigen in stool sample by ICT (Immunochromatographic test) as well as to observe the seasonal variation of rotavirus infection. This cross sectional study was carried out in the department of Microbiology, Dhaka Medical College from January 2011 to December 2011. Eighty stool samples were collected from Dhaka Shishu Hospital and Dhaka Medical College Hospital. All samples were tested for rotavirus antigen by ICT. Among 80 patients, 42 (52.5%) samples were positive for rotavirus antigen. Among these 42 positive samples, 30 (71.43%) were from 0-12 months of age group, 10 (23.81%) from 13 to 24 months of age group and rest 2 (4.76%) from 25 to 36 months of age group. Rotavirus Ag was detected in stool samples from January to April and another peak episode from October to December. Considering the importance of Rotavirus associated diarrhea, rapid detection of Rotavirus infection in human is substantially needed and should be routinely practiced.DOI: http://dx.doi.org/10.3329/bjmm.v6i1.19354 Bangladesh J Med Microbiol 2012; 06(01): 11-13

An Epidemiological, Diagnostic and Therapeutic Study of Giardia lamblia in Anbar Province – Iraq

2019 ◽  
Vol 9 (01) ◽  
pp. 39-45
Author(s):  
Suad Shallal Shahatha
Keyword(s):  
Teaching Hospital ◽  
Rural Areas ◽  
Giardia Lamblia ◽  
Urban Areas ◽  
Culture Media ◽  
Age Groups ◽  
Stool Samples ◽  
Women And Children ◽  
Teucrium Polium L ◽  
Therapeutic Study

This study was carried out to investigate the epidemiology of Giardia lamblia parasites in patients who visited some of the hospitals in Anbar province, which included (Fallujah Teaching Hospital, Ramadi Teaching Hospital, Ramadi Teaching Hospital for Women and Children and Hit Hospital) during by examining 864 stool samples in a direct examination method, The results revealed the infection rate was 41.7 % and the percentage of infection among males 47.8% is higher than that of females 35.4% with significant differences (p≤0.05). The age groups (1-9) years recorded the highest rates 55.4% and the lowest rate 13.6% in the age group (40-49) years. The highest rate of infection was 62.5% during the month of June, while the month of October was the lowest rate 5% and significant differences. The incidence rate in rural areas was 50.6% higher than in the urban areas 32.5%. The study also included the effect of Teucrium polium L. on the parasite in the culture media HSP-1, the concentrations of 0.5-3 mg / mL significantly affected Giardia, it was noted whenever the greater the concentration, the greater the effect during different treatment periods (1-4) days, as the highest concentration 3 mg/ml killed all Giardia parasites on the fourth day of treatment.

Hepatic Encephalopathy and Liver Failure in Pediatric Patient with hepatitis A

2019 ◽  
Vol 9 (o3) ◽  
Author(s):  
Shaima’a Dakhel AbdulHassa
Keyword(s):  
Hepatic Encephalopathy ◽  
Liver Failure ◽  
Hepatitis A ◽  
Gene Sequence ◽  
Stool Examination ◽  
Age Group ◽  
Significant Difference ◽  
Medical City ◽  
Sequence Method ◽  
Stool Samples

Gairdia lamblia is one of parasites that cause intestinal problems within the human body, particularly private travelers and children. In this study a total of (100) diarrheal patients, 20 patients with Giardiasis were identified by fecal antigen. 9 out of 20(20%) of them were infected by fecal antigen, while 9(9%) of them were infected by using the screening general stool examination (GSE). The stool samples were collected from patient how vested the Medical City/ Baghdad and Tikrit teaching Hospital during the period from 1 st may 2018 to 1 February 2019. The results revealing a significant difference (p andlt; 0.05) between the two methods of detection for G. lamblia (Fecal antigen method and GSE). IT has been shown that out of 20 infected individuals 12(12%) were males and 8(8%) were females, indicating regarding no significant deference in the distribution of Giardiasis among genders. In regard the age, our results showed that highest infection rate 8(3.2%) was recorded in the age group (10-19) years, followed by the age group (20-2) years which was 692.4%). In this study five mutations were recorded at position (926, 1094, 1202and 1304), by using tpiA gene sequence method, and tpiB gene was on point mutation change (G254A), in the position (85) of triose phosphate isomease.

Sign in / Sign up

Export Citation Format

Share Document