scholarly journals Initial velocities in situ of G6PDH and PGDH and expression of proliferating cell nuclear antigen (PCNA): sensitive diagnostic markers of environmentally induced hepatocellular carcinogenesis in a marine flatfish (Platichthys flesus L.)

1998 ◽  
Vol 40 (2-3) ◽  
pp. 233-252 ◽  
Author(s):  
Angela Köhler ◽  
Cornelis J.F. Van Noorden
Keyword(s):  
Proliferating Cell Nuclear Antigen ◽  
Diagnostic Markers ◽  
Nuclear Antigen ◽  
Platichthys Flesus ◽  
Hepatocellular Carcinogenesis ◽  
Proliferating Cell

scholarly journals In Situ Analysis of Cellular Proliferation in Canine, Feline and Equine Tumors by Immunohistochemistry: A Comparison of Bromodeoxyuridine, Proliferating Cell Nuclear Antigen, and Interchromatin-Associated Antigen Immunostaining Techniques

1994 ◽  
Vol 6 (4) ◽  
pp. 453-457 ◽  
Author(s):  
Alain Pierre Théon ◽  
Loretta Metzger ◽  
Stephen Griffey
Keyword(s):  
Cell Proliferation ◽  
Proliferating Cell Nuclear Antigen ◽  
Cellular Proliferation ◽  
Nuclear Antigen ◽  
Brdu Incorporation ◽  
Immunohistochemical Detection ◽  
Proliferating Cells ◽  
Proliferating Cell

Cell proliferation in canine, feline, and equine tumors was evaluated using immunohistochemical detection of in vitro 5–bromodeoxyuridine (BrdU) incorporation, proliferating cell nuclear antigen (PCNA), and interchromatin-associated antigen (p105). Ten tumors in each species were analyzed. The tumor proliferative fraction (PF) was defined as the percentage of labeled nuclei for 5,000 tumor nuclei counted. Immunoreactivity was observed with all techniques in all species. A good correlation was observed between the proliferative fractions measured with the BrdU (PFBrdU) and PCNA (PFPCNA) techniques ( rs = 0.523, P = 0.0026). There was no correlation between the PFs measured with the BrdU (PFBrdU) and p105 (PFP105) techniques. Using the median values obtained from the different approaches as cutoff points to define slowly and rapidly proliferating tumors, there was an 80% agreement ( P = 0.009) between PFBrdU and PFPCNA and no agreement between PFBrdU and PFP105 The results of this study indicate that both BrdU and PCNA labeling methods can be used reliably for identifying proliferating cells in animal tumors. In addition, PCNA could be used to replace the BrdU method to assess tumor proliferative fraction because it does not require pretreatment of tissues.

Determination of cell proliferative activity by immnunohistochemical detection of proliferating cell nuclear antigen in N‐methyl nitrosoureainduced rat mammary tumours

2013 ◽  
Vol 3 (4) ◽  
pp. 188-194
Author(s):  
R. Barathidasan ◽  
R. V. S. Pawaiya ◽  
R. B. Rai ◽  
K. Dhama
Keyword(s):  
Papillary Carcinoma ◽  
Proliferating Cell Nuclear Antigen ◽  
Nuclear Antigen ◽  
Nuclear Staining ◽  
Chemical Carcinogen ◽  
Malignant Tumours ◽  
Mammary Tumours ◽  
Proliferating Cell

Rodent experimental models are considered useful experimental systems to study mammary cancer, which occurs in rodents as a result of hormonal imbalance, much like the breast cancer in women. Rodent cells are easier to transform by chemical carcinogen due to their relatively poorer controls of genetic stability and DNA repair systems. Proliferating cell nuclear antigen (PCNA) is one of the most sensitive biomarkers to measure the cell proliferative activities in the neoplasms, especially when the otherwise undetectable PCNA protein over‐expresses to the immunohistochemically detectable levels. In the present study, mammary tumours were induced in Sprague Dawley rats by intraperitoneal administration of N‐methyl nitrosourea (MNU) chemical carcinogen. Palpable tumours were noticed after 60 days postinjection (dpi) with average latency period of 144 days, and 12 (46.15%) of 26 rats developed mammary tumours. A total of 18 tumours were diagnosed in 12 rats, including 2 cases (10%) of hyperplasia, 1 (5%) benign and 17 malignant tumours (85%). Malignant tumours included in situ ductal papillary carcinoma (11.11%), in situ ductal cribriform carcinoma (38.88%), invasive tubular adenocarcinoma (5.56%), invasive papillary carcinoma (16.67%) and invasive cribriform carcinoma (22.22%) were. PCNA immunohistochemistry revealed moderate to strong nuclear staining in neoplastic cells, with highest mean PCNA index of 55.53±10.13 in invasive cribriform carcinoma. The PCNA indices were significantly different (p<0.01; one way ANOVA) between control mammary gland, hyperplasia, benign and malignant tumours. It was concluded that the measurement of PCNA expressing cells employing immunohistochemistry can help in the determination of the level of malignancy in MNU‐induced rat mammary tumours.

Nuclear DNA content, proliferating-cell nuclear antigen (PCNA) and p53 immunostaining in predicting progression of laryngeal cancer in situ lesions

2007 ◽  
Vol 56 (1) ◽  
pp. 95-99 ◽  
Author(s):  
Eva Munck-Wikland ◽  
Staffan Edström ◽  
Eva Jungmark ◽  
Richard Kuylenstierna ◽  
Johan Lindholm ◽  
...  
Keyword(s):  
Laryngeal Cancer ◽  
Dna Content ◽  
Proliferating Cell Nuclear Antigen ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Nuclear Antigen ◽  
Proliferating Cell

scholarly journals Expression of Piwi Genes during the Regeneration of Lineus sanguineus (Nemertea, Pilidiophora, Heteronemertea)

Genes ◽  
2020 ◽  
Vol 11 (12) ◽  
pp. 1484
Author(s):  
Cong-Mei Xu ◽  
Shi-Chun Sun
Keyword(s):  
Stem Cells ◽  
Proliferating Cell Nuclear Antigen ◽  
Direct Evidence ◽  
Nuclear Antigen ◽  
Regeneration Capacity ◽  
Pluripotent Cells ◽  
Self Renewal ◽  
Proliferating Cell ◽  
Anterior Regeneration

The transposon silencer piwi genes play important roles in germline determination and maintenance, gametogenesis, and stem-cell self-renewal, and the expression of certain piwi genes is indispensable for regeneration. Knowledge about piwi genes is needed for phylum Nemertea, which contains members (e.g., Lineus sanguineus) with formidable regeneration capacity. By searching the L. sanguineus genome, we identified six Argonaute genes including three ago (Ls-Ago2, Ls-Ago2a, and Ls-Ago2b) and three piwi (Ls-piwi1, Ls-piwi2, and Ls-piwi3) genes. In situ hybridization revealed that, in intact females, Ls-piwi2 and Ls-piwi3 were not expressed, while Ls-piwi1 was expressed in ovaries. During regeneration, Ls-piwi1 and Ls-pcna (proliferating cell nuclear antigen) had strong and similar expressions. The expression of Ls-piwi1 became indetectable while Ls-pcna continued to be expressed when the differentiation of new organs was finished. During anterior regeneration, expression signals of Ls-piwi2 and Ls-piwi3 were weak and only detected in the blastema stage. During posterior regeneration, no expression was observed for Ls-piwi2. To date, no direct evidence has been found for the existence of congenital stem cells in adult L. sanguineus. The “pluripotent cells” in regenerating tissues are likely to be dedifferentiated from other type(s) of cells.

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