Rapid detection of columnaris disease in channel catfish (Ictalurus punctatus) with a new species-specific 16-S rRNA gene-based PCR primer for Flavobacterium columnare

2003 ◽  
Vol 52 (2) ◽  
pp. 209-220 ◽  
Author(s):  
Joel A Bader ◽  
Craig A Shoemaker ◽  
Phillip H Klesius
Keyword(s):  
New Species ◽  
Ictalurus Punctatus ◽  
Channel Catfish ◽  
Rapid Detection ◽  
Rrna Gene ◽  
Flavobacterium Columnare ◽  
Columnaris Disease ◽  
Species Specific ◽  
16 S Rrna ◽  
A New Species

scholarly journals Draft Genome Sequences of Flavobacterium columnare Strains ARS1 and BGFS27, Isolated from Channel Catfish (Ictalurus punctatus)

2019 ◽  
Vol 8 (26) ◽  
Author(s):  
Wenlong Cai ◽  
C. R. Arias
Keyword(s):  
Ictalurus Punctatus ◽  
Channel Catfish ◽  
Draft Genome ◽  
Genetic Group ◽  
Flavobacterium Columnare ◽  
Genome Sequences ◽  
Content Type ◽  
Columnaris Disease ◽  
Group 3 ◽  
Group 2

Flavobacterium columnare strain BGFS27 was isolated from an apparently healthy wild channel catfish (Ictalurus punctatus) collected from the Mobile River in 2005. F. columnare strain ARS1 was isolated from a channel catfish suffering from columnaris disease in a commercial farm in 1996. BGFS27 belongs to genomovar II (genetic group 2), while ARS1 belongs to genomovar III (genetic group 3). Here, we report the draft genome sequences of F. columnare BGFS27 and ARS1, obtained by PacBio sequencing.

Description of Tylenchulus musicola sp. n. (Nematoda: Tylenchulidae) from banana in Iran with molecular phylogeny and characterisation of species of Tylenchulus Cobb, 1913

Nematology ◽  
2012 ◽  
Vol 14 (3) ◽  
pp. 353-369 ◽  
Author(s):  
Zahra Tanha Maafi ◽  
Majid Amani ◽  
Jason D. Stanley ◽  
Renato N. Inserra ◽  
Esther Van den Berg ◽  
...  
Keyword(s):  
New Species ◽  
Rapid Identification ◽  
Rrna Genes ◽  
Rrna Gene ◽  
28S Rrna ◽  
Specific Primers ◽  
Diagnostic Pcr ◽  
Second Stage ◽  
Species Specific ◽  
A New Species

During a survey conducted on banana plantations in Sistan and Blouchestan province, south-east Iran, a new species of Tylenchulus was extracted from the soil and roots of banana plants. This species, named Tylenchulus musicola sp. n., is characterised by mature females having a swollen, hook-shaped body with a conical and elongate post-vulval portion ending in a round terminus, males having a weak stylet and a cylindrical and thick tail ending in a bluntly rounded and smooth terminus, and by second-stage juveniles having a slender body and a posterior body portion ending in a finely pointed or mucronate terminus. The results of glasshouse host tests indicated that the new species does not parasitise sugarcane ratoons or sour orange seedlings. Tylenchulus musicola sp. n. is distinguished from other known Tylenchulus species by the sequences of D2-D3 expansion segments of 28S rRNA and ITS rRNA genes. Phylogenetic relationships within Tylenchulus were reconstructed based on rRNA gene sequences using Bayesian inference. Diagnostic PCR-ITS-RFLP profiles are presented for T. musicola sp. n., T. furcus, T. graminis, T. palustris, T. semipenetrans and Trophotylenchulus floridensis. PCR with species-specific primers and genus-specific primer are tested and developed for rapid identification of five Tylenchulus species. An identification key to Tylenchulus species is provided.

Crepidobothrium fragile n.sp., a Tapeworm of the Channel Catfish

Parasitology ◽  
1929 ◽  
Vol 21 (1-2) ◽  
pp. 164-167
Author(s):  
Hiram E. Essex
Keyword(s):  
New Species ◽  
Ictalurus Punctatus ◽  
Channel Catfish ◽  
Intestinal Parasites ◽  
Fish Host ◽  
Entire Family ◽  
Complete Knowledge ◽  
The Family ◽  
A New Species

While investigating the species of Corallobothrium (Essex, 1928) I examined the intestinal parasites of 180 channel catfish (Ictalurus punctatus) taken in Rock River (Illinois). Only six of these fish harboured tapeworms that could not be assigned to the genus Corallobothrium. From the six fish, besides the species of Corallobothrium, I found fifteen adult Cestodes belonging to the genus Crepidobothrium. A study of these parasites has shown that they constitute a new species for which I propose the name Crepidobothrium fragile, because of the ease with which the strobila is broken. The members of this genus have been reported previously only from amphibians and reptiles. This is, so far as I am aware, the first recorded occurrence of a species of Crepidobothrium from a fish host. Nybelin (1917) has shown that the genus Ophiotaenia (La Rue, 1911) is synonymous with Crepidobothrium. It has been pointed out by Woodland (1925, 1925a) that the characters used by La Rue (1914) as the basis for the separation of the genera of Proteocephalids have been unreliable because of their inconstancy. He has proposed that all the genera of the family Proteocephalidae be placed in the genus Proteocephalus. Meggitt (1927) has indicated that this procedure is too sweeping and if followed would make “a most heterogeneous assemblage of some 100 species.” Therefore, he has placed all these species under four genera, namely, Corallobothrium, Crepidobothrium, Gangesia and Ichthyotaenia (= Proteocephalus), all of which have been well established for a number of years, and greater confusion of the literature may be avoided if they are retained until some worker, in the light of more complete knowledge, again makes a revision of the entire family. Since there yet remains some doubt as to the characters that will ultimately prove trustworthy in the classification of the Proteocephalids, descriptions of new species should be as complete as possible. Although there is much variability in the measurements of a given species of Cestode, yet the fact should not be overlooked that the range of size of the organism and its parts is of considerable aid in classification. It is admitted that there is great variability in size in a given species of Cestode, but if the extremes are known the size becomes of significant value.

Bioeconomic Analysis of Flavobacterium columnare Vaccine Pond Trials with Channel Catfish Ictalurus punctatus

2021 ◽  
Author(s):  
Jillian K. Malecki ◽  
Luke A. Roy ◽  
Cova R. Arias ◽  
Miles D. Lange ◽  
Craig A. Shoemaker ◽  
...  
Keyword(s):  
Ictalurus Punctatus ◽  
Channel Catfish ◽  
Flavobacterium Columnare

Description of a new species of Auriculostoma (Digenea: Allocreadiidae) from Characidium heirmostigmata (Characiformes: Crenuchidae) from Argentina, using morphological and molecular data

2021 ◽  
Vol 95 ◽  
Author(s):  
M.M. Montes ◽  
J. Barneche ◽  
Y. Croci ◽  
D. Balcazar ◽  
A. Almirón ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
National Park ◽  
Molecular Data ◽  
Rrna Gene ◽  
28S Rrna ◽  
The Family ◽  
Parasitological Survey ◽  
Morphological And Molecular Data ◽  
A New Species

Abstract During a parasitological survey of fishes at Iguazu National Park, Argentina, specimens belonging to the allocreadiid genus Auriculostoma were collected from the intestine of Characidium heirmostigmata. The erection of the new species is based on a unique combination of morphological traits as well as on phylogenetic analysis. Auriculostoma guacurarii n. sp. resembles four congeneric species – Auriculostoma diagonale, Auriculostoma platense, Auriculostoma tica and Auriculostoma totonacapanensis – in having smooth and oblique testes, but can be distinguished by a combination of several morphological features, hosts association and geographic distribution. Morphologically, the new species can be distinguished from both A. diagonale and A. platense by the egg size (bigger in the first and smaller in the last); from A. tica by a shorter body length, the genital pore position and the extension of the caeca; and from A. totonacapanensis by the size of the oral and ventral sucker and the post-testicular space. Additionally, one specimen of Auriculostoma cf. stenopteri from the characid Charax stenopterus (Characiformes) from La Plata River, Argentina, was sampled and the partial 28S rRNA gene was sequenced. The phylogenetic analysis revealed that A. guacurarii n. sp. clustered with A. tica and these two as sister taxa to A. cf. stenopteri. The new species described herein is the tenth species in the genus and the first one parasitizing a member of the family Crenuchidae.

Steinernema borjomiense n. sp. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from Georgia

Nematology ◽  
2018 ◽  
Vol 20 (7) ◽  
pp. 653-669 ◽  
Author(s):  
Oleg Gorgadze ◽  
Elena Fanelli ◽  
Manana Lortkhipanidze ◽  
Alberto Troccoli ◽  
Medea Burjanadze ◽  
...  
Keyword(s):  
New Species ◽  
18S Rrna ◽  
Entomopathogenic Nematode ◽  
Phylogenetic Analyses ◽  
Tail Length ◽  
Morphological Characters ◽  
18S Rrna Gene ◽  
The Body ◽  
Rrna Gene ◽  
A New Species

Summary A new species of entomopathogenic nematode, Steinernema borjomiense n. sp., was isolated from the body of the host insect, Oryctes nasicornis (Coleoptera: Scarabaeidae), in Georgia, in the territory of Borjomi-Kharagauli. Morphological characters indicate that the new species is closely related to species of the feltiae-group. The infective juveniles are characterised by the following morphological characters: body length of 879 (777-989) μm, distance between the head and excretory pore = 72 (62-80) μm, pharynx length = 132 (122-142) μm, tail length = 70 (60-80) μm, ratio a = 26.3 (23.0-29.3), H% = 45 (40-51), D% = 54 (47-59), E% = 102 (95-115), and lateral fields consisting of seven ridges (eight incisures) at mid-body. Steinernema borjomiense n. sp. was molecularly characterised by sequencing three ribosomal regions (the ITS, the D2-D3 expansion domains and the 18S rRNA gene) and the mitochondrial COI gene. Phylogenetic analyses revealed that S. borjomiense n. sp. differs from all other known species of Steinernema and is a member of the monticolum-group.

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