scholarly journals EP-1654: Clinical set up and first results of EPID in vivo dosimetry in an overload Chinese Radiotherapy

2017 ◽  
Vol 123 ◽  
pp. S898-S899
Author(s):  
J. Li ◽  
A. Piermattei ◽  
P. Wang ◽  
S. Kang ◽  
M. Xiao ◽  
...  
Keyword(s):  
In Vivo Dosimetry ◽  
First Results ◽  
Set Up

OC-0065 A NATIONAL PROJECT FOR THE IN-VIVO DOSIMETRY IN RADIOTHERAPY: FIRST RESULTS

2012 ◽  
Vol 103 ◽  
pp. S26
Author(s):  
A. Piermattei ◽  
L. Azario ◽  
S. Cilla ◽  
A. Fidanzio ◽  
F. Greco ◽  
...  
Keyword(s):  
In Vivo Dosimetry ◽  
National Project ◽  
First Results

A National project for in vivo dosimetry procedures in radiotherapy: First results

2012 ◽  
Vol 274 ◽  
pp. 42-50 ◽  
Author(s):  
Angelo Piermattei ◽  
Francesca Greco ◽  
Luigi Azario ◽  
Andrea Porcelli ◽  
Savino Cilla ◽  
...  
Keyword(s):  
In Vivo Dosimetry ◽  
National Project ◽  
First Results

scholarly journals EP-1647: Validation of a set up procedure for IMRT/VMAT breast treatment using in vivo dosimetry with EPID

2017 ◽  
Vol 123 ◽  
pp. S894
Author(s):  
S. Kang ◽  
J. Li ◽  
P. Wang ◽  
X. Liao ◽  
M. Xiao ◽  
...  
Keyword(s):  
In Vivo Dosimetry ◽  
Set Up

Continuous Quantitative Monitoring of Mural, Platelet-Dependent, Thrombus Kinetics in the Crushed Rat Femoral Vein

1991 ◽  
Vol 65 (04) ◽  
pp. 425-431 ◽  
Author(s):  
F Stockmans ◽  
H Deckmyn ◽  
J Gruwez ◽  
J Vermylen ◽  
R Acland
Keyword(s):  
Thrombus Formation ◽  
Femoral Vein ◽  
Maximum Size ◽  
Digital Analysis ◽  
Video Recordings ◽  
Quantitative Monitoring ◽  
Set Up ◽  
Kinetics Of ◽  
Quantitative Nature

SummaryA new in vivo method to study the size and dynamics of a growing mural thrombus was set up in the rat femoral vein. The method uses a standardized crush injury to induce a thrombus, and a newly developed transilluminator combined with digital analysis of video recordings. Thrombi in this model formed rapidly, reaching a maximum size 391 ± 35 sec following injury, after which they degraded with a half-life of 197 ± 31 sec. Histological examination indicated that the thrombi consisted mainly of platelets. The quantitative nature of the transillumination technique was demonstrated by simultaneous measurement of the incorporation of 111In labeled platelets into the thrombus. Thrombus formation, studied at 30 min interval in both femoral veins, showed satisfactory reproducibility overall and within a given animalWith this method we were able to induce a thrombus using a clinically relevant injury and to monitor continuously and reproducibly the kinetics of thrombus formation in a vessel of clinically and surgically relevant size

Rapid, Refined, and Robust Method for Expression, Purification, and Characterization of Recombinant Human Amyloid-beta M1-42

2019 ◽  
Author(s):  
Priya Prakash ◽  
Travis Lantz ◽  
Krupal P. Jethava ◽  
Gaurav Chopra
Keyword(s):  
Amyloid Beta ◽  
Western Blots ◽  
Force Microscopy ◽  
E Coli ◽  
Atomic Force ◽  
Set Up ◽  
Short Time

Amyloid plaques found in the brains of Alzheimer’s disease (AD) patients primarily consists of amyloid beta 1-42 (Ab42). Commercially, Ab42 is synthetized using peptide synthesizers. We describe a robust methodology for expression of recombinant human Ab(M1-42) in Rosetta(DE3)pLysS and BL21(DE3)pLysS competent E. coli with refined and rapid analytical purification techniques. The peptide is isolated and purified from the transformed cells using an optimized set-up for reverse-phase HPLC protocol, using commonly available C18 columns, yielding high amounts of peptide (~15-20 mg per 1 L culture) in a short time. The recombinant Ab(M1-42) forms characteristic aggregates similar to synthetic Ab42 aggregates as verified by western blots and atomic force microscopy to warrant future biological use. Our rapid, refined, and robust technique to purify human Ab(M1-42) can be used to synthesize chemical probes for several downstream in vitro and in vivo assays to facilitate AD research.

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