Co-ordination of photosynthetic gene expression during low-temperature acclimation and development in Brassica napus cv. Jet Neuf leaves

Plant Science ◽  
1998 ◽  
Vol 135 (2) ◽  
pp. 171-181 ◽  
Author(s):  
Minati Singh ◽  
Anne Johnson-Flanagan
Keyword(s):  
Gene Expression ◽  
Brassica Napus ◽  
Low Temperature ◽  
Temperature Acclimation ◽  
Photosynthetic Gene

Temporal and spatial distribution of BN28 during low temperature acclimation of Brassica napus cv. Cascade seedlings

1997 ◽  
Vol 75 (1) ◽  
pp. 28-35
Author(s):  
Mitchel D. de Beus ◽  
Anne M. Johnson-Flanagan ◽  
Joseph G. Boothe
Keyword(s):  
Brassica Napus ◽  
Low Temperature ◽  
Leaf Age ◽  
Temporal Distribution ◽  
Leaf Tissue ◽  
Temperature Acclimation ◽  
Ground Tissue ◽  
Temporal And Spatial Distribution ◽  
Apical Meristems ◽  
Mature Leaves

The objective of this study was to determine the spatial and temporal distribution of the peptide BN28 in Brassica napus cv. Cascade seedlings during low temperature acclimation. Immunoblots revealed that BN28 was present in leaves and shoot apical meristems of plants grown under low temperature but was absent from older tissues in the stem. Immunocytochemistry was used to determine the distribution throughout leaf and apical meristem tissues. BN28 was found throughout the apical meristems, was localized in the ground tissue and epidermis of young leaves, and was restricted to the ground tissue and guard cells in mature leaves. Differences in total accumulation were also noted, with the youngest leaves having the highest accumulation of BN28 and the quantity decreasing with leaf age. Despite these differences, plant developmental stage did not affect the accumulation of BN28 in individual leaves. Post-transcriptional controls are expected to regulate accumulation of the protein, as bn28 mRNA accumulates during acclimation in both young and mature leaves. Immunolocalization studies of BN28 in acclimated leaf tissue confirmed that BN28 is cytoplasmically localized and has no apparent weak association with organelles or other cellular membrane systems. Key words: acclimation, Brassica, development, immunocytochemistry, low temperature, protein synthesis.

scholarly journals Unravelling the Complex Interplay of Transcription Factors Orchestrating Seed Oil Content in Brassica napus L.

2021 ◽  
Vol 22 (3) ◽  
pp. 1033
Author(s):  
Abirami Rajavel ◽  
Selina Klees ◽  
Johanna-Sophie Schlüter ◽  
Hendrik Bertram ◽  
Kun Lu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transcription Factors ◽  
Brassica Napus ◽  
Oil Content ◽  
Seed Oil ◽  
Seed Oil Content ◽  
Complex Interplay ◽  
Brassica Napus L ◽  
Data Set ◽  
Developmental Switches

Transcription factors (TFs) and their complex interplay are essential for directing specific genetic programs, such as responses to environmental stresses, tissue development, or cell differentiation by regulating gene expression. Knowledge regarding TF–TF cooperations could be promising in gaining insight into the developmental switches between the cultivars of Brassica napus L., namely Zhongshuang11 (ZS11), a double-low accession with high-oil- content, and Zhongyou821 (ZY821), a double-high accession with low-oil-content. In this regard, we analysed a time series RNA-seq data set of seed tissue from both of the cultivars by mainly focusing on the monotonically expressed genes (MEGs). The consideration of the MEGs enables the capturing of multi-stage progression processes that are orchestrated by the cooperative TFs and, thus, facilitates the understanding of the molecular mechanisms determining seed oil content. Our findings show that TF families, such as NAC, MYB, DOF, GATA, and HD-ZIP are highly involved in the seed developmental process. Particularly, their preferential partner choices as well as changes in their gene expression profiles seem to be strongly associated with the differentiation of the oil content between the two cultivars. These findings are essential in enhancing our understanding of the genetic programs in both cultivars and developing novel hypotheses for further experimental studies.

scholarly journals Analysis of Transcriptional Changes in Different Brassica napus Synthetic Allopolyploids

Genes ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 82
Author(s):  
Yunxiao Wei ◽  
Guoliang Li ◽  
Shujiang Zhang ◽  
Shifan Zhang ◽  
Hui Zhang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Brassica Napus ◽  
Differentially Expressed Genes ◽  
Expression Patterns ◽  
Female Parent ◽  
Enrichment Analysis ◽  
Differentially Expressed ◽  
Transcriptional Changes ◽  
Aa Genome ◽  
High Degree

Allopolyploidy is an evolutionary and mechanistically intriguing process involving the reconciliation of two or more sets of diverged genomes and regulatory interactions, resulting in new phenotypes. In this study, we explored the gene expression patterns of eight F2 synthetic Brassica napus using RNA sequencing. We found that B. napus allopolyploid formation was accompanied by extensive changes in gene expression. A comparison between F2 and the parent shows a certain proportion of differentially expressed genes (DEG) and activation\silent gene, and the two genomes (female parent (AA)\male parent (CC) genomes) showed significant differences in response to whole-genome duplication (WGD); non-additively expressed genes represented a small portion, while Gene Ontology (GO) enrichment analysis showed that it played an important role in responding to WGD. Besides, genome-wide expression level dominance (ELD) was biased toward the AA genome, and the parental expression pattern of most genes showed a high degree of conservation. Moreover, gene expression showed differences among eight individuals and was consistent with the results of a cluster analysis of traits. Furthermore, the differential expression of waxy synthetic pathways and flowering pathway genes could explain the performance of traits. Collectively, gene expression of the newly formed allopolyploid changed dramatically, and this was different among the selfing offspring, which could be a prominent cause of the trait separation. Our data provide novel insights into the relationship between the expression of differentially expressed genes and trait segregation and provide clues into the evolution of allopolyploids.

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