Expression of murine soluble CD4 protein in baculovirus infected insect cells

Immunobiology ◽  
1992 ◽  
Vol 186 (3-4) ◽  
pp. 254-267 ◽  
Author(s):  
Jörg Kupsch ◽  
Kai M. Saizawa ◽  
Klaus Eichmann
Keyword(s):  
Insect Cells ◽  
Infected Insect ◽  
Soluble Cd4

scholarly journals Production, processing and partial purification of functional G protein βγ subunits in baculovirus-infected insect cells

1992 ◽  
Vol 286 (3) ◽  
pp. 677-680 ◽  
Author(s):  
J D Robishaw ◽  
V K Kalman ◽  
K L Proulx
Keyword(s):  
G Protein ◽  
G Proteins ◽  
Insect Cells ◽  
Expression System ◽  
Baculovirus Expression System ◽  
Partial Purification ◽  
Baculovirus Expression ◽  
Gamma Subunits ◽  
Infected Insect ◽  
Beta 2

As a result of the inability to resolve the heterogeneous mixture of G protein beta gamma subunits present in tissues, it has not been possible to compare different beta gamma subunits of the G proteins in terms of their proposed roles in receptor-effector coupling. This study was undertaken to establish the utility of the baculovirus expression system in producing homogeneous beta gamma subunits of defined composition for the comparative analysis of these subunits in reconstitution systems. In this study we report the expression, and appropriate post-translational processing, of recombinant beta 2, gamma 2 and gamma 3 subunits. In addition, we show that the recombinant beta gamma subunits can be readily purified, and can functionally interact with the alpha subunits of the G proteins.

Expression and Characterization of Human Dopamine D2Receptor in Baculovirus-Infected Insect Cells

1994 ◽  
Vol 14 (2) ◽  
pp. 99-117 ◽  
Author(s):  
J. A. Javitch ◽  
J. Kaback ◽  
X. Li ◽  
A. Karlin
Keyword(s):  
Insect Cells ◽  
Infected Insect

Purification and characterization of truncated human AMPK α2β2γ3 heterotrimer from baculovirus-infected insect cells

2010 ◽  
Vol 70 (1) ◽  
pp. 13-22 ◽  
Author(s):  
Lata Ramanathan ◽  
Payal R. Sheth ◽  
Polina Ogas ◽  
Li Xiao ◽  
Hung V. Le
Keyword(s):  
Insect Cells ◽  
Purification And Characterization ◽  
Infected Insect

High-level production of biologically active human cytosolic phospholipase A2 in baculovirus-infected insect cells

Gene ◽  
1993 ◽  
Vol 128 (2) ◽  
pp. 307-308 ◽  
Author(s):  
Bernard Y. Amegadzie ◽  
Deborah Jiampetti ◽  
Robert J. Craig ◽  
Edward Appelbaum ◽  
Allan R. Shatzman ◽  
...  
Keyword(s):  
Phospholipase A2 ◽  
Insect Cells ◽  
Cytosolic Phospholipase A2 ◽  
Biologically Active ◽  
Cytosolic Phospholipase ◽  
Infected Insect ◽  
High Level ◽  
Level Production
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