Sucrose Transport into, and Unloading from, the Seed Coat of Empty Ovules of Pisum sativum over Time-Spans Greater than Three Hours

1992 ◽  
Vol 140 (6) ◽  
pp. 687-690
Author(s):  
Pieter Wolswinkel ◽  
Judith W. Koerselman-Kooij
Keyword(s):  
Pisum Sativum ◽  
Seed Coat ◽  
Sucrose Transport ◽  
Over Time

scholarly journals Anatomy and Histochemistry of Seed Coat Development of Wild (Pisum sativum subsp. elatius (M. Bieb.) Asch. et Graebn. and Domesticated Pea (Pisum sativum subsp. sativum L.)

2021 ◽  
Vol 22 (9) ◽  
pp. 4602
Author(s):  
Lenka Zablatzká ◽  
Jana Balarynová ◽  
Barbora Klčová ◽  
Pavel Kopecký ◽  
Petr Smýkal
Keyword(s):  
Pisum Sativum ◽  
Seed Coat ◽  
Developmental Stages ◽  
Histochemical Staining ◽  
Ruthenium Red ◽  
Detailed Knowledge ◽  
Maternal Plant ◽  
Wild Progenitor ◽  
Strong Signal ◽  
Biochemical Studies

In angiosperms, the mature seed consists of embryo, endosperm, and a maternal plant-derived seed coat (SC). The SC plays a role in seed filling, protects the embryo, mediates dormancy and germination, and facilitates the dispersal of seeds. SC properties have been modified during the domestication process, resulting in the removal of dormancy, mediated by SC impermeability. This study compares the SC anatomy and histochemistry of two wild (JI64 and JI1794) and two domesticated (cv. Cameor and JI92) pea genotypes. Histochemical staining of five developmental stages: 13, 21, 27, 30 days after anthesis (DAA), and mature dry seeds revealed clear differences between both pea types. SC thickness is established early in the development (13 DAA) and is primarily governed by macrosclereid cells. Polyanionic staining by Ruthenium Red indicated non homogeneity of the SC, with a strong signal in the hilum, the micropyle, and the upper parts of the macrosclereids. High peroxidase activity was detected in both wild and cultivated genotypes and increased over the development peaking prior to desiccation. The detailed knowledge of SC anatomy is important for any molecular or biochemical studies, including gene expression and proteomic analysis, especially when comparing different genotypes and treatments. Analysis is useful for other crop-to-wild-progenitor comparisons of economically important legume crops.

Hugo field pea

2009 ◽  
Vol 89 (1) ◽  
pp. 73-75 ◽  
Author(s):  
Deng-Jin Bing ◽  
Don Beauchesne ◽  
Al Sloan ◽  
Yantai Gan ◽  
Cecil Vera ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Pisum Sativum ◽  
Seed Coat ◽  
Field Pea ◽  
Western Canada ◽  
Seed Shape ◽  
Leaf Type ◽  
Pisum Sativum L ◽  
Cultivar Description ◽  
Round Seed

Hugo is a high-yielding field pea (Pisum sativum L.) cultivar with yellow cotyledons. It has a semi-leafless leaf type, and is powdery mildew resistant. It has round seed shape, medium seed size and high seed coat integrity. The cultivar is adapted to field pea growing regions in western Canada. Key words: Field pea, Pisum sativum, cultivar description

Dormancy and Viability in Acacia suaveolens (Sm) Willd

1986 ◽  
Vol 34 (4) ◽  
pp. 463 ◽  
Author(s):  
TD Auld
Keyword(s):  
Seed Coat ◽  
Fruit Ripening ◽  
Plant Age ◽  
Time Space ◽  
High Level ◽  
Tetrazolium Test ◽  
Over Time

Germination in A. suaveolens was controlled by a seed coat-linked innate dormancy, and scarification was successful in breaking this dormancy in the laboratory. The tetrazolium test was a useful indicator of potential germination capability. Such a baseline for germination is necessary before comparisons between different pre-germination treatments can be validly attempted both inter and intraspecifically. The onset of innate dormancy in the field occurs at the time of fruit ripening and most seeds are dispersed in a state of innate dormancy (96 � 0.5%). This level of dormancy and a correspondingly high level of viability (93.8 � 1.5%) are constant over time, space and plant age.

scholarly journals Anatomical and micromorphological characteristics of the seed coat of field pea (Pisum sativum L.) genotypes in relation to cracks and damage of seeds

2017 ◽  
Vol 69 (3) ◽  
pp. 503-512 ◽  
Author(s):  
Jelena Lazarevic ◽  
Lana Zoric ◽  
Djura Karagic ◽  
Branko Milosevic ◽  
Dunja Karanovic ◽  
...  
Keyword(s):  
Pisum Sativum ◽  
Cross Sections ◽  
Seed Coat ◽  
Morphological Characteristics ◽  
Average Diameter ◽  
Seed Length ◽  
Damage Level ◽  
Pisum Sativum L ◽  
Multivariate Discriminant ◽  
Micromorphological Analysis

In this paper, we present the morphological characteristics of the seed and micromorphological, anatomical and chemical characteristics of the seed coat of pea (Pisum sativum L.) genotypes, Jezero, Javor and NS Junior. Our aim was to investigate whether these genotypes can be differentiated based on seed coat morphoanatomical characteristics, depending on the harvest treatment. The observations and measurements of seed coat cross-sections were performed using light microscopy. The seed coat surface was observed using SEM. A tuberculate seed coat surface characterized all examined pea genotypes, and the average diameter of the tubercle was about 12 ?m. Statistical and laboratory analyses revealed that major damage was the most frequent defect type as the result of mechanized harvest in all the examined genotypes. Genotype NS Junior had the shortest seed length (6.1 mm). Micromorphological analysis revealed that the seed surface was tuberculate in all genotypes. The genotype Jezero had the highest number of tubercle ribs (11.0) and a significantly higher proportion of parenchyma tissue (50.6%), while NS Junior was characterized by the greatest share of macrosclereids (49.8%). The highest number of osteosclereids (832/mm2) was counted in genotype Javor. In addition, genotype NS Junior stands out due to the highest percentage of crude fiber (62.75 g/100g) in the seed coat. There was a marked difference among the studied genotypes with regard to the seed coat morphoanatomical characteristics, which is confirmed by the results of multivariate discriminant analysis (MDA). These results suggested that the morphological, micromorphological and anatomical characteristics of the seed might have an impact on the seed coat damage level at harvest.

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