A Tissue-Culture Derived Salt-Tolerant Line of Flax (Linum usitatissimum)

1984 ◽  
Vol 117 (2) ◽  
pp. 109-117 ◽  
Author(s):  
A. McHughen ◽  
M. Swartz
Keyword(s):  
Tissue Culture ◽  
Linum Usitatissimum ◽  
Salt Tolerant ◽  
Tolerant Line

FIELD EVALUATION ON NONSALINE SOILS OF A SOMACLONAL VARIANT OF McGREGOR FLAX SELECTED FOR SALT TOLERANCE IN VITRO

1988 ◽  
Vol 68 (2) ◽  
pp. 345-349 ◽  
Author(s):  
G. G. ROWLAND ◽  
A. McHUGHEN ◽  
C. McONIE
Keyword(s):  
Tissue Culture ◽  
Linum Usitatissimum ◽  
Oil Content ◽  
Field Experiments ◽  
Salient Feature ◽  
Field Evaluation ◽  
Salt Tolerant ◽  
Somaclonal Variant ◽  
Early Maturing

Field experiments, comparing the performance of a tissue-culture-derived salt-tolerant selection (STS) of McGregor flax (Linum usitatissimum) with the cultivars McGregor, Linott, Noralta, NorLin and NorMan, were sown at three nonsaline locations in Saskatchewan in 1984 and 1985. The salient feature of this experiment was the dissimilarity of STS to its parent, McGregor. STS yielded less, was earlier to bolt, flower and mature, and had larger seeds with a lower oil content than McGregor. The yield of STS was similar to other early-maturing cultivars under test.Key words: Linum usitatissimum, somaclonal variation, seed yield

Selection of Salt-Tolerant Plants Using Tissue Culture

1980 ◽  
pp. 279-292 ◽  
Author(s):  
D. W. Rains ◽  
T. P. Croughan ◽  
S. J. Stavarek
Keyword(s):  
Tissue Culture ◽  
Salt Tolerant ◽  
Tolerant Plants ◽  
Selection Of

scholarly journals Comparative transcriptome analyses of maize seedling root responses to salt stress

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e10765
Author(s):  
Xiaoxiang Zhang ◽  
Peng Liu ◽  
Chunyan Qing ◽  
Cong Yang ◽  
Yaou Shen ◽  
...  
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Maize Seedling ◽  
Salt Tolerant ◽  
Maize Roots ◽  
Differential Expression Genes ◽  
Salt Response ◽  
Seedling Roots ◽  
Tolerant Line ◽  
Sensitive Line

Salt stress affects crop yield by limiting growth and delaying development. In this study, we constructed 16 transcriptome libraries from maize seedling roots using two maize lines, with contrasting salt tolerance, that were exposed to salt stress for 0, 6, 18 and 36 h. In total, 6,584 differential expression genes (DEGs; 3,669 upregulated, 2,915 downregulated) were induced in the salt-sensitive line and 6,419 DEGs (3,876 upregulated, 2,543 downregulated) were induced in the salt-tolerant line. Several DEGs common to both lines were enriched in the ABA signaling pathway, which was presumed to coordinate the process of maize salt response. A total of 459 DEGs were specifically induced in the salt-tolerant line and represented candidate genes responsible for high salt-tolerance. Expression pattern analysis for these DEGs indicated that the period between 0 and 6 h was a crucial period for the rapid response of the tolerant genes under salt stress. Among these DEGs, several genes, Aux/IAA, SAUR, and CBL-interacting kinase have been reported to regulate salt tolerance. In addition, the transcription factors WRKY, bZIP and MYB acted as regulators in the salt-responsive regulatory network of maize roots. Our findings will contribute to understanding of the mechanism on salt response and provide references for functional gene revelation in plants.

Development of salt tolerant lines of KDML and LPT rice cultivars through tissue culture

1989 ◽  
Vol 8 (7) ◽  
pp. 411-414 ◽  
Author(s):  
M. Vajrabhaya ◽  
T. Thanapaisal ◽  
T. Vajrabhaya
Keyword(s):  
Tissue Culture ◽  
Rice Cultivars ◽  
Salt Tolerant

Phenolic content and antioxidant activity in two contrasting Medicago ciliaris lines cultivated under salt stress

Biologia ◽  
2011 ◽  
Vol 66 (5) ◽  
Author(s):  
Imène Salah ◽  
Héla Mahmoudi ◽  
Margaret Gruber ◽  
Tarek Slatni ◽  
Mondher Boulaaba ◽  
...  
Keyword(s):  
Salt Stress ◽  
Leaf Growth ◽  
Soluble Sugars ◽  
Total Biomass ◽  
Salt Tolerant ◽  
Total Soluble Sugars ◽  
Micronutrient Status ◽  
Medicago Ciliaris ◽  
Tolerant Line ◽  
Sensitive Line

AbstractThe objective of this study was to determine more indepth physiological and antioxidant responses in two Medicago ciliaris lines (a salt-tolerant line TNC 1.8 and a salt-sensitive line TNC 11.9) with contrasting responses to 100 mM NaCl. Under salt stress, both lines showed a decrease in total biomass and in the growth rate for roots, but TNC 1.8 was less affected by salt than TNC 11.9 in that it maintained leaf growth even in the presence of added salt. In both lines, salt stress mainly affected micronutrient status (Fe, Mn, Cu and Zn) rather than K nutrition, but the tolerant line TNC 1.8 accumulated more Na in leaves and less in roots compared with TNC 11.9. Salt stress decreased total soluble sugars (TSS) in all organs of the sensitive line TNC 11.9, whereas TSS was only reduced in roots of the tolerant line. The salt-induced drop in growth was linked to an increase in lipid peroxidation in roots of both lines and in leaves of the sensitive line. The salt-tolerant line TNC 1.8 was more efficient at managing salt-induced oxidative damage in leaves and to a lesser extent in roots than the salt-sensitive line TNC 11.9, by preserving higher phenolic compound and superoxide dismutase levels in both organs.

FIELD PERFORMANCE AT SALINE-AFFECTED SITES OF A SOMACLONAL VARIANT OF McGREGOR FLAX SELECTED FOR SALT TOLERANCE IN VITRO

1989 ◽  
Vol 69 (1) ◽  
pp. 49-60 ◽  
Author(s):  
G. G. ROWLAND ◽  
A. McHUGHEN ◽  
C. McONIE
Keyword(s):  
Seed Yield ◽  
Linum Usitatissimum ◽  
Dry Matter ◽  
Field Performance ◽  
Dry Matter Yield ◽  
Plant Establishment ◽  
Salt Tolerant ◽  
Somaclonal Variant ◽  
Salinity Response

The response to salinity of a tissue culture derived, salt-tolerant selection (STS) of flax (Linum usitatissimum) was measured at three saline-affected field sites in Saskatchewan in 1985 and 1986. Two experiments were conducted at each site in each year. Experiment A compared the plant population, dry matter yield and seed yield of STS to its parent cultivar McGregor. Experiment B compared the plant establishment, growth rates, days to flower, days to mature and seed yield per plant of STS, McGregor and the cultivar Noralta which has a maturity similar to STS. The seed yield of McGregor was greater than that of STS in both saline and nonsaline conditions and was significantly less affected by salinity than STS. However, when equal numbers of seeds were sown, more STS plants survived to maturity than either McGregor or Noralta. The decrease in seed yield per plant with increasing salinity was usually greater for McGregor and Noralta than STS and sometimes significantly so. The maturity differences among McGregor, STS and Noralta were not correlated with the differences in seed yield per plant. There was no consistent pattern in the results to suggest that STS was more saline-tolerant than were flax cultivars developed using conventional breeding procedures.Key words: Linum usitatissimum, somaclonal variation, salinity response

scholarly journals Constitutive Overexpression of the Plasma Membrane Na+/H+ Antiporter for Conferring Salinity Tolerance in Rice

2016 ◽  
Vol 25 (2) ◽  
pp. 257-272 ◽  
Author(s):  
Farida Yasmin ◽  
Sudip Biswas ◽  
GM Nurnabi Azad Jewel ◽  
Sabrina M Elias ◽  
Zeba I Seraj
Keyword(s):  
Tissue Culture ◽  
Plasma Membrane ◽  
Indica Rice ◽  
Rice Variety ◽  
Segregation Ratio ◽  
Transformation Method ◽  
Cellular Level ◽  
Salt Tolerant ◽  
In Planta ◽  
Rice Transformation

At the cellular level, the Salt Overly Sensitive (SOS) signaling pathway comprising SOS3, SOS2, and SOS1 has been proposed to mediate cellular signaling under salt stress to maintain ion (Na+) homeostasis. In this regulatory pathway, both OsSOS1 encoding plasma membrane and OsNHX1 encoding vacuolar Na+/H+ antiporters are regulated by SOS3?SOS2 protein kinase complex. In the present study, the rice variety BRRI dhan28 - which is popular with farmers and high yielding, but salt sensitive, was transformed with the OsSOS1 gene isolated from salt tolerant Pokkali rice and driven by the constitutive promoter, CaMV35S. The construct was transformed through a tissue culture-independent Agrobacteriummediated in planta transformation method that circumvents the problems associated with tissue culture-based indica rice transformation methods. Integration of the foreign genes (OsSOS1) into the genome of transgenic plants was confirmed by gene-specific PCR and Southern blot analysis. The level of transgene expression (SOS1) was also quantified by semi-quantitative RT PCR and real time PCR. Genetic segregation ratio for T1 progenies was calculated and found to follow the Mendelian law of inheritance in case of positive transformants. The transformants were shown to be salt tolerant compared to wild type in molecular analysis as well as physiological screening. Future work will involve transformation of both the OsSOS1 and OsNHX1 genes together; with the expectation for enhancing the tolerance level compared to currently available transgenic rice.Plant Tissue Cult. & Biotech. 25(2): 257-272, 2015 (December)

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