P3156 CD14 C(-260)T polymorphism, plasma levels of soluble CD14, their associations with changes in arterial wall

2003 ◽  
Vol 24 (5) ◽  
pp. 588
Author(s):  
J AMAR
Keyword(s):  
Plasma Levels ◽  
Arterial Wall ◽  
Soluble Cd14

Lack of association between the –260 C→T promoter polymorphism of the endotoxin receptor CD14 gene and the CD14 density of unstimulated human monocytes and soluble CD14 plasma levels

2001 ◽  
Vol 27 (11) ◽  
pp. 1770-1775 ◽  
Author(s):  
Michael Heesen ◽  
Brunhilde Blömeke ◽  
Bernhard Schlüter ◽  
Nicole Heussen ◽  
Rolf Rossaint ◽  
...  
Keyword(s):  
Plasma Levels ◽  
Promoter Polymorphism ◽  
Human Monocytes ◽  
Soluble Cd14 ◽  
Cd14 Gene

scholarly journals Selected Biomarkers Correlate with the Origin and Severity of Sepsis

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Michal Holub ◽  
Olga Džupová ◽  
Michaela Růžková ◽  
Alžběta Stráníková ◽  
Eva Bartáková ◽  
...  
Keyword(s):  
Intensive Care Unit ◽  
Plasma Levels ◽  
Plasma Concentrations ◽  
Community Acquired Pneumonia ◽  
Heparin Binding ◽  
Soluble Cd14 ◽  
Gram Negative ◽  
Heparin Binding Protein ◽  
Microbial Etiology ◽  
Time Point

The microbial etiology and source of sepsis influence the inflammatory response. Therefore, the plasma levels of cytokines (IL-6, IL-8, and IL-10), chemokines (CCL2/MCP-1, MIP-1β), heparin-binding protein (HBP), soluble CD14 (sCD14), and cortisol were analyzed in blood from septic patients obtained during the first 96 hours of intensive care unit hospitalization. The etiology was established in 56 out of a total of 62 patients enrolled in the study. Plasma concentrations of MCP-1, sCD14, IL-6, and IL-10 were significantly higher in patients with community-acquired pneumonia (CAP;n=10) and infective endocarditis (IE;n=11) compared to those with bacterial meningitis (BM;n=18). Next, cortisol levels were higher in IE patients than in those with BM and CAP, and at one time point, cortisol was also higher in patients with gram-negative sepsis when compared to those with gram-positive infections. Furthermore, cortisol and MCP-1 levels correlated positively with the daily measured SOFA score. In addition, HBP levels were significantly higher in patients with IE than in those with BM. Our findings suggest that MCP-1, sCD14, IL-6, IL-10, cortisol, and HBP are modulated by the source of sepsis and that elevated MCP-1 and cortisol plasma levels are associated with sepsis-induced organ dysfunction.

scholarly journals Plasma levels of the arterial wall protein fibulin-1 are associated with carotid-femoral pulse wave velocity: a cross-sectional study

2013 ◽  
Vol 12 (1) ◽  
pp. 107 ◽  
Author(s):  
Esben Laugesen ◽  
Pernille Høyem ◽  
Jens Christiansen ◽  
Søren Knudsen ◽  
Klavs Hansen ◽  
...  
Keyword(s):  
Pulse Wave Velocity ◽  
Wave Velocity ◽  
Plasma Levels ◽  
Arterial Wall ◽  
Pulse Wave ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Cross Sectional

scholarly journals Plasma Levels of Soluble CD14 Independently Predict Mortality in HIV Infection

2011 ◽  
Vol 203 (6) ◽  
pp. 780-790 ◽  
Author(s):  
Netanya G. Sandler ◽  
Handan Wand ◽  
Annelys Roque ◽  
Matthew Law ◽  
Martha C. Nason ◽  
...  
Keyword(s):  
Hiv Infection ◽  
Plasma Levels ◽  
Soluble Cd14

scholarly journals Correlation between arterial wall stiffness, N-terminal prohormone of brain natriuretic peptide, functional and structural myocardial abnormalities in patients with type 2 diabetes mellitus and cardiac autonomic neuropathy

2013 ◽  
Vol 16 (4) ◽  
pp. 72-77
Author(s):  
Viktoriya Alexandrovna Serhiyenko ◽  
Alexander Alexandrovich Serhiyenko ◽  
Boris Nikitich Mankovsky
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Brain Natriuretic Peptide ◽  
Plasma Levels ◽  
Arterial Wall ◽  
Vascular Wall ◽  
Cardiac Autonomic Neuropathy ◽  
Wall Stiffness

Aim. To assess arterial wall stiffness, plasma levels of of N-terminal prohormone of brain natriuretic peptide (NT-proBNP), as well as functional state and structure of the myocardium in patients with type 2 diabetes mellitus (T2DM) and cardiac autonomic neuropathy (CAN). Materials and Methods. The study involved a total of 65 patients with T2DM. 12 had no evidence of cardiovascular disease (CVD) or CAN, 14 were diagnosed with subclinical stage of CAN, 18 ? with functional stage, and 21 ? with organic stage. We measured aortic pulse wave velocity (PWV), aortic augmentation index (AIx), brachial artery AIx, ambulatory arterial stiffness index (AASI) and plasma levels of NT-proBNP. Clinical examination included ECG, Holter monitoring, ambulatory BP measurement and echocardiography. Results.  Patients with isolated T2DM showed a trend for increased vascular wall stiffness. PWV was increased in patients with subclinical stage of CAN. Aortic and brachial AIx, PWV and AASI were elevated in patients with functional stage of CAN, PWV being significantly higher vs. subclinical CAN subgroup. Organic stage was characterized by pathologically increased values of all primary parameters; PWV and AASI were significantly higher compared with other groups. Development and progression of CAN was accompanied by an increase in NT-proBNP plasma levels. Concentration of NT-proBNP was in direct correlation with left ventricular mass (LVM) and PWV. PWV and LVM values also directly correlated between themselves. Conclusion. Development and progression of CAN in patients with T2DM is accompanied by an increase in vascular wall stiffness. The elevation of plasma NT-proBNP in patients with T2DM correlates with the development of CAN and is significantly and independently associated with an increase in LVM and PWV. Our data suggests the pathophysiological interconnection between metabolic, functional and structural myocardial abnormalities in patients with T2DM and CAN.

scholarly journals CD14 (C-159T) polymorphism is associated with increased susceptibility to SLE, and plasma levels of soluble CD14 is a novel biomarker of disease activity: A hospital-based case-control study

Lupus ◽  
2020 ◽  
pp. 096120332097279
Author(s):  
Aditya K Panda ◽  
Rina Tripathy ◽  
Bidyut K Das
Keyword(s):  
Lupus Nephritis ◽  
Disease Activity ◽  
Plasma Levels ◽  
Enzyme Linked Immunosorbent Assay ◽  
Healthy Controls ◽  
Systemic Lupus ◽  
Soluble Cd14 ◽  
Functional Variants ◽  
Tnf Α ◽  
Kidney Involvement

Background Cluster of differentiation 14 (CD14) plays a crucial role in the innate immune response of the host in protection against various pathogens. The importance of soluble CD14 in autoimmune disorders has been described in different populations. However, the role of sCD14 in systemic lupus erythematosus (SLE) is poorly understood. Further, the association of functional variants at the promoter region of the CD14 gene (−159 C > T) with susceptibility to SLE or disease severity needs to be defined. Methods Two hundred female SLE patients diagnosed on systemic lupus international collaborating clinics (SLICC) classification criteria and age, sex, matched healthy controls were enrolled in the present study. Polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP) method was used to genotype CD14 (C-159 T) polymorphism. Plasma levels of IFN-α, TNF-α, and sCD14 were quantified by enzyme-linked immunosorbent assay (ELISA). Results Prevalence of mutant genotypes (CT and TT) and minor allele (T) of CD14 (C-159T) polymorphism was significantly higher in SLE cases compared to healthy controls (CT: P < 0.0001; OR = 3.26, TT:P < 0.0001; OR = 3.39; T:P = 0.0009, OR = 1.62). Further, lupus nephritis patients had a higher prevalence of homozygous mutants (TT) and mutant allele (T)(TT: P = 0.0002, OR = 8.07; T: P = 0.001, OR = 1.32). SLE patients displayed significantly increased plasma sCD14, TNF-α, and IFN-α levels in comparison to healthy controls. These cytokines were significantly elevated in patients of lupus nephritis compared to those without kidney involvement. Interestingly, sCD14 levels correlated positively with SLE disease activity index-2K (SLEDAI-2K) scores and 24 hours proteinuria. Conclusion CD14 (C-159T) polymorphism is associated with an increased predisposition to the development of SLE and lupus nephritis: sCD14 is a promising novel biomarker for assessing disease activity and lupus nephritis.

scholarly journals Elevated Levels of Lipopolysaccharide-Binding Protein and Soluble CD14 in Plasma in Neonatal Early-Onset Sepsis

2002 ◽  
Vol 9 (2) ◽  
pp. 440-445 ◽  
Author(s):  
Reinhard Berner ◽  
Birgitt Fürll ◽  
Felix Stelter ◽  
Jana Dröse ◽  
Hans-Peter Müller ◽  
...  
Keyword(s):  
Cord Blood ◽  
Plasma Levels ◽  
Early Onset ◽  
Binding Protein ◽  
Control Group ◽  
Lipopolysaccharide Binding Protein ◽  
Soluble Cd14 ◽  
Early Onset Sepsis ◽  
Cytokine Plasma ◽  
Lipopolysaccharide Binding

ABSTRACT No data on lipopolysaccharide-binding protein (LBP) in newborns with sepsis have been available up to now. We therefore determined levels of LBP and soluble CD14 (sCD14) in plasma of healthy and septic neonates in order to evaluate their potential diagnostic role. The study included prospectively collected patient samples of two recently published studies on cytokine expression in neonatal sepsis. Twenty-nine septic patients were enrolled in the present analysis. Samples—either cord blood or peripheral blood—from patients admitted within the first 24 h of life for suspicion of sepsis and cord blood samples of a control group of 40 healthy mature infants delivered spontaneously were analyzed. For seven patients of the septic group, a second sample collected between 24 and 48 h of life was available. Levels of sCD14 and LBP in plasma were determined by an enzyme immunoassay using recombinant CD14 and LBP as standards. LBP and sCD14 were correlated to cytokine plasma levels. In septic neonates, LBP (median, 36.6 versus 7.8 μg/ml; P < 0.001) and sCD14 (median, 0.42 versus 0.28 μg/ml; P < 0.001) levels were highly elevated when compared to those of healthy neonates and strongly correlated to granulocyte colony-stimulating factor (G-CSF), interleukin-1β (IL-1β), IL-6, and IL-8 levels. LBP levels in septic neonates analyzed between 24 and 48 h of life even increased when compared to samples obtained at or shortly after delivery (median, 36.6 versus 60 μg/ml; P = 0.038). In summary, levels of LBP in plasma of neonates with early-onset sepsis are significantly elevated; the elevated plasma levels seem to persist for more than 24 h, which could provide the clinician with a prolonged time period to identify the newborn with bacterial sepsis.

scholarly journals Changes in Endotoxin-Binding Proteins during Major Elective Surgery: Important Role for Soluble CD14 in Regulation of Biological Activity of Systemic Endotoxin

1999 ◽  
Vol 6 (6) ◽  
pp. 844-850 ◽  
Author(s):  
Naoki Hiki ◽  
Dieter Berger ◽  
Mieke A. Dentener ◽  
Yoshikazu Mimura ◽  
Wim A. Buurman ◽  
...  
Keyword(s):  
Abdominal Surgery ◽  
Plasma Levels ◽  
Binding Proteins ◽  
Biologically Active ◽  
Major Abdominal Surgery ◽  
Human Model ◽  
High Density Lipoproteins ◽  
Soluble Cd14 ◽  
Plasma Endotoxin ◽  
Neutralizing Capacity

ABSTRACT Assessment of circulating endotoxin during the perioperative period, which is only demonstrated by the Limulus amebocyte lysate (LAL) test, may be modulated by several endotoxin-binding proteins. Endotoxin-neutralizing capacity (ENC) and the plasma levels of soluble CD14 (sCD14), lipopolysaccharide-binding protein, and bactericidal/permeability-increasing protein (BPI) were determined in 40 patients 6 h prior to skin incision for major abdominal surgery. The bioactivity of plasma endotoxin was tested by the polymyxin B-inhibited stimulatory activity of the plasma samples on healthy monocytes as measured by the release of tumor necrosis factor alpha. Plasma endotoxin levels in almost all patients increased from 0.05 ± 0.01 to 0.23 ± 0.03 experimental units (EU) per ml (P < 0.001); more specifically, 17 of 40 samples showed endotoxin levels of greater than 0.2 EU per ml and corresponding reductions in ENC. Soluble CD14 plasma levels were decreased from 5.6 ± 0.3 to 4.6 ± 0.3 μg per ml (P < 0.05). ENC was strongly correlated with the sCD14 plasma concentration throughout the period of observation. The addition of sCD14-neutralizing monoclonal anti-sCD14 antibodies reduced ENC both pre- and postoperatively. No correlation could be established between ENC and the plasma levels of BPI, high-density lipoproteins, or low-density lipoproteins determined by measuring the concentrations of apoprotein A and apoprotein B. Biologically active endotoxin was found in only 6 of 17 samples with endotoxin levels greater than 0.2 EU per ml in the LAL test. These samples could be characterized by their perioperative loss of at least 35% of their sCD14. No change in sCD14 was detected in the remaining 11 samples. The perioperative loss of ENC is partly caused by the loss of sCD14 resulting from its consumption by endotoxin reaching the bloodstream. This study demonstrated the role of sCD14 on the bioactivity of circulating endotoxin in a human model of endotoxemia after major abdominal surgery.

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