Characterization of monoclonal antibodies directed against the bovine herpesvirus-1 glycoprotein E and use for the differentiation between vaccinated and infected animals

2001 ◽  
Vol 83 (4) ◽  
pp. 301-315 ◽  
Author(s):  
C Letellier ◽  
A Delangre ◽  
A De Smet ◽  
P Kerkhofs
Keyword(s):  
Monoclonal Antibodies ◽  
Bovine Herpesvirus ◽  
Bovine Herpesvirus 1 ◽  
Glycoprotein E ◽  
Herpesvirus 1

Molecular Characterization of South American Bovine Herpesvirus-1 Isolates with Monoclonal Antibodies and SDS-PAGE

1993 ◽  
Vol 40 (1-10) ◽  
pp. 125-130 ◽  
Author(s):  
A. Suarez Heinlein ◽  
A. E. Metzler ◽  
R. Weiblen ◽  
P. Berrios ◽  
A. A. Schudel ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Molecular Characterization ◽  
Bovine Herpesvirus ◽  
South American ◽  
Bovine Herpesvirus 1 ◽  
Sds Page ◽  
Herpesvirus 1

Characterization of monoclonal antibodies against bovine herpesvirus 1 gD fusion protein expressed in E. coli

1999 ◽  
Vol 83 (1-2) ◽  
pp. 83-89 ◽  
Author(s):  
Japhet R.S Lyaku ◽  
Patricia K McKenna ◽  
Robert A Fredrickson ◽  
Frederick S.B Kibenge
Keyword(s):  
Monoclonal Antibodies ◽  
Fusion Protein ◽  
Bovine Herpesvirus ◽  
Bovine Herpesvirus 1 ◽  
E Coli ◽  
Herpesvirus 1

Biological characterization of bovine herpesvirus 1 recombinants possessing the vaccine glycoprotein E negative phenotype

2006 ◽  
Vol 113 (3-4) ◽  
pp. 283-291 ◽  
Author(s):  
Benoît Muylkens ◽  
François Meurens ◽  
Frédéric Schynts ◽  
Katalin de Fays ◽  
Aldo Pourchet ◽  
...  
Keyword(s):  
Bovine Herpesvirus ◽  
Biological Characterization ◽  
Bovine Herpesvirus 1 ◽  
Glycoprotein E ◽  
Herpesvirus 1 ◽  
Negative Phenotype

scholarly journals Construction and Manipulation of an Infectious Clone of the Bovine Herpesvirus 1 Genome Maintained as a Bacterial Artificial Chromosome

2002 ◽  
Vol 76 (13) ◽  
pp. 6660-6668 ◽  
Author(s):  
Timothy J. Mahony ◽  
Fiona M. McCarthy ◽  
Jennifer L. Gravel ◽  
Lani West ◽  
Peter L. Young
Keyword(s):  
Bacterial Artificial Chromosome ◽  
Infectious Clone ◽  
Bovine Herpesvirus ◽  
Artificial Chromosome ◽  
Gene Encoding ◽  
Kinase Gene ◽  
Bovine Herpesvirus 1 ◽  
Glycoprotein E ◽  
Herpesvirus 1

ABSTRACT The complete genome of bovine herpesvirus 1 (BoHV-1) strain V155 has been cloned as a bacterial artificial chromosome (BAC). Following electroporation into Escherichia coli strain DH10B, the BoHV-1 BAC was stably propagated over multiple generations of its host. BAC DNA recovered from DH10B cells and transfected into bovine cells produced a cytopathic effect which was indistinguishable from that of the parent virus. Analysis of the replication kinetics of the viral progeny indicated that insertion of the BAC vector into the thymidine kinase gene did not affect viral replication. Specific manipulation of the BAC was demonstrated by deleting the gene encoding glycoprotein E by homologous recombination in DH10B cells facilitated by GET recombination. These studies illustrate that the propagation and manipulation of herpesviruses in bacterial systems will allow for rapid and accurate characterization of BoHV-1 genes. In turn, this will allow for the full utilization of BoHV-1 as a vaccine vector.

EvaGreen-based Multiplex Real-time PCR Assay for Rapid Differentiation of Wild-Type and Glycoprotein E-Deleted Bovine Herpesvirus-1 Strains

2017 ◽  
Vol 28 (4) ◽  
pp. 248-252 ◽  
Author(s):  
Sachin S. Pawar ◽  
Chetan D. Meshram ◽  
Niraj K. Singh ◽  
Mohini Saini ◽  
B. P. Mishra ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Bovine Herpesvirus ◽  
Wild Type ◽  
Pcr Assay ◽  
Bovine Herpesvirus 1 ◽  
Glycoprotein E ◽  
Herpesvirus 1

scholarly journals Safety and immunogenicity of a glycoprotein E gene-deleted bovine herpesvirus 1 strain as a candidate vaccine strain

2016 ◽  
Vol 36 (11) ◽  
pp. 1067-1074
Author(s):  
Marcelo Weiss ◽  
◽  
Deniz Anziliero ◽  
Mathias Martins ◽  
Rudi Weiblen ◽  
...  
Keyword(s):  
Acute Infection ◽  
Clinical Signs ◽  
Bovine Herpesvirus ◽  
Elisa Test ◽  
Bovine Herpesvirus 1 ◽  
Glycoprotein E ◽  
Virus Shedding ◽  
Group I ◽  
Virus Dose ◽  
Herpesvirus 1

ABSTRACT: A glycoprotein E-deleted Brazilian bovine herpesvirus 1 (BoHV-1gEΔ) was tested regarding to safety and immunogenicity. Intramuscular inoculation of young calves with a high virus dose did not result in clinical signs or virus shedding during acute infection or after dexamethasone administration. Calves vaccinated once IM (group I) or subcutaneously (group II) with live BoHV-1gEΔ or twice with inactivated virus plus aluminum hydroxide (group IV) or Montanide™ (group V) developed VN titers of 2 to 8 (GMT:2); 2 to 4 (GMT:1.65); 2 to 16 (GMT:2.45) and 2 to 128 (GMT:3.9), respectively. All BoHV-1gEΔ vaccinated calves remained negative in an anti-gE ELISA. Lastly, six young calves vaccinated with live BoHV-1gEΔ and subsequently challenged with a virulent BoHV-1 strain shed less virus and developed only mild and transient nasal signs comparing to unvaccinated calves. Thus, the recombinant BoHV-1gEΔ is safe and immunogenic for calves and allows for serological differentiation by a gE-ELISA test.

Sign in / Sign up

Export Citation Format

Share Document