Simple and sensitive high-performance liquid chromatographic method for the determination of 1,5-benzodiazepine clobazam and its active metabolite N-desmethylclobazam in human serum and urine with application to 1,4-benzodiazepines analysis

2001 ◽  
Vol 750 (1) ◽  
pp. 41-49 ◽  
Author(s):  
Paweł K Kunicki
Keyword(s):  
Human Serum ◽  
Active Metabolite ◽  
High Performance ◽  
Chromatographic Method ◽  
High Performance Liquid Chromatographic ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic ◽  
Serum And Urine

A High-Performance Liquid Chromatographic Method for the Determination of Meropenem in Serum

2019 ◽  
Vol 58 (2) ◽  
pp. 144-150
Author(s):  
Demet Dincel ◽  
Olcay Sagirli ◽  
Gulacti Topcu
Keyword(s):  
Human Serum ◽  
High Performance ◽  
Chromatographic Method ◽  
Acetic Acid Solution ◽  
High Performance Liquid Chromatographic ◽  
Array Detector ◽  
Therapeutic Drug ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic

Abstract In this study, a new, sensitive and selective high-performance liquid chromatographic method was developed for the determination of meropenem (MEM) in human serum. In the developed method, C18 column (3.9 × 150 mm, 5 μm) was selected as stationary phase at 30°C, and methanol: acetic acid solution mixture was used as mobile phase with gradient program. Chromatographic separation was carried out at a flow rate of 1 mL/min, and detection was performed at 300 nm with diode array detector. Doripenem was selected as an internal standard, and the analytes were extracted from serum using protein precipitation method with ortho-phosphoric acid: methanol. Detection wavelength was selected as 300 nm. The developed method was validated according to International Council for Harmonisation (ICH) guidelines. The calibration curve was linear over a concentration range of 4–240 μg/mL with correlation coefficient of 0.9985. The limit of detection and limit of quantification values were found as 0.057 and 0.192 μg/mL, respectively. The validated method was successfully applied for the determination of MEM in human serum samples collected from patient volunteers at different time intervals, and therapeutic drug monitoring of MEM has been investigated.

Fluoroimmunoassays for procainamide and N-acetylprocainamide compared with a liquid-chromatographic method.

1984 ◽  
Vol 30 (5) ◽  
pp. 768-773 ◽  
Author(s):  
J J MacKichan ◽  
J D Coyle ◽  
B J Shields ◽  
H Boudoulas ◽  
J J Lima
Keyword(s):  
Excellent Agreement ◽  
Pharmacokinetic Study ◽  
Active Metabolite ◽  
High Performance ◽  
Chromatographic Method ◽  
High Performance Liquid Chromatographic ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic

Abstract We measured procainamide and its active metabolite, N-acetylprocainamide (NAPA), in 80 sera from 37 patients by a new fluorimmunoassay procedure and an established "high-performance" liquid-chromatographic method. Additive and proportional differences between the methods were 0.07 mg/L and 9%, respectively, for procainamide and 0.62 mg/L and 16% for NAPA. Between-day CVs by the chromatographic and immunoassay methods, respectively, were 3.9% and 2.2% for procainamide at a concentration of 6 mg/L, and 5.1% and 1.2% for NAPA (14 mg/L). We applied a modification of the fluoroimmunoassay for determination of procainamide concentrations, using sera obtained during a pharmacokinetic study, and demonstrated excellent agreement with the chromatographic method.

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