Separation and measurement of tricyclic antidepressant drugs in plasma by high-performance liquid chromatography

1977 ◽  
Vol 143 (5) ◽  
pp. 535-539 ◽  
Author(s):  
R.R. Brodie ◽  
L.F. Chasseaud ◽  
D.R. Hawkins
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Antidepressant Drugs ◽  
Tricyclic Antidepressant ◽  
Tricyclic Antidepressant Drugs

scholarly journals Transformation of Amoxapine byCunninghamella elegans

2000 ◽  
Vol 66 (8) ◽  
pp. 3646-3649 ◽  
Author(s):  
Joanna D. Moody ◽  
Donglu Zhang ◽  
Thomas M. Heinze ◽  
Carl E. Cerniglia
Keyword(s):  
Mass Spectrometry ◽  
Nuclear Magnetic Resonance ◽  
High Performance Liquid Chromatography ◽  
Biological Activity ◽  
Liquid Chromatography ◽  
Magnetic Resonance ◽  
High Performance ◽  
Tricyclic Antidepressant ◽  
Cunninghamella Elegans ◽  
Toxicological Evaluation

ABSTRACT We examined Cunninghamella elegans to determine its ability to transform amoxapine, a tricyclic antidepressant belonging to the dibenzoxazepine class of drugs. Approximately 57% of the exogenous amoxapine was metabolized to three metabolites that were isolated by high-performance liquid chromatography and were identified by nuclear magnetic resonance and mass spectrometry as 7-hydroxyamoxapine (48%),N-formyl-7-hydroxyamoxapine (31%), andN-formylamoxapine (21%). 7-Hydroxyamoxapine, a mammalian metabolite with biological activity, now can be produced in milligram quantities for toxicological evaluation.

scholarly journals Laboratory toxicological diagnosis of trazodone intoxications

2021 ◽  
Vol 25 (1) ◽  
pp. 57-62
Author(s):  
S. A. Karpushyna ◽  
S. V. Baiurka ◽  
S. I. Merzlikin
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Methylene Chloride ◽  
Antidepressant Drug ◽  
Antidepressant Drugs ◽  
Liquid Extraction ◽  
Spectrophotometric Detection ◽  
Trichloroacetic Acid Solution ◽  
Toxicological Studies

When determining the cause of poisoning by antidepressant drugs, the data of laboratory toxicological studies of biofluids for the presence of this group of drugs are of key importance. Aim. To develop the methods for determining the antidepressant drug trazodone in the blood and urine samples using high-performance liquid chromatography with a UV spectrophotometric detection, which is suitable for analytical diagnosis of the thymoleptics intoxications. Materials and methods. The model samples of human biofluids spiked with trazodone were studied. The antidepressant was isolated from the blood and urine by the liquid-liquid extraction with methylene chloride from the alkaline medium at pH 9. Concomitant endogenous impurities were removed by extraction with diethyl ether from the acidic medium at pH 1. In the study of the blood the erythrocyte mass was pre-precipitated with the help of 10 % of trichloroacetic acid solution. Chromatographic analysis was performed on a microcolumn chromatograph using a column with a reversed-phase of C18. Results. The absolute retention time of trazodone in extracts from the model samples of biofluids was 17.91±0.09 min. The quantitative content of trazodone was determined at 250 nm by the calibration dependence of the chromatographic peak area on the concentration (μg/ml) y=(1.74∙10-3±1∙10-5)x. Under the indicated extraction conditions, 35±4 % and 78±4 % of trazodone were isolated from the blood and urine, respectively. Conclusions. The methods of trazodone isolation from the biofliuds by liquid extraction followed by the determination of the drug by high performance liquid chromatography with a multiwave UV spectrophotometric detection have been developed. The methods are recommended for using in the practice of forensic and clinical toxicology.

Liquid-chromatographic analysis for common tricyclic antidepressant drugs and their metabolites in serum or plasma with the technicon "FAST-LC" system.

1981 ◽  
Vol 27 (6) ◽  
pp. 849-855 ◽  
Author(s):  
S J Bannister ◽  
S van der Wal ◽  
J W Dolan ◽  
L R Snyder
Keyword(s):  
High Performance ◽  
Antidepressant Drugs ◽  
Total Sample ◽  
Sample Volume ◽  
Tricyclic Antidepressant ◽  
Tricyclic Antidepressant Drugs ◽  
Liquid Chromatographic Analysis ◽  
Double Extraction ◽  
Liquid Chromatographic ◽  
Total Sample Volume

Abstract We describe a single procedure for assay of seven tricyclic antidepressant drugs and metabolites in serum or plasma: protriptyline, nortriptyline, amitriptyline, desmethyldoxepin, doxepin, desipramine, and imipramine. With the Technicon "FAST-LC" system, samples are aspirated directly into the unit and pretreated via double extraction; the concentration of each drug is then determined by "high-performance" liquid chromatography. Final chromatograms are monitored at 205 nm, at analysis rates of 7.5 samples/h. Concentration and absorbance are linearly related for each drug from 0 to 1400 micrograms/L. Day-to-day CVs averaged 5 to 6% for each drug, and there is good correlation of FAST-LC values with those obtained by gas-chromatographic methods. Total sample volume is 750 microliters.

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