Proliferation of peripheral blood mononuclear cells causes increased expression of the sodium-dependent multivitamin transporter gene and increased uptake of pantothenic acid ☆ ☆This work was supported by National Institutes of Health grant DK 36823, USDA/CSREES award 2001-35200-10187, and a grant from the College of Medicine at the University of Arkansas for Medical Sciences.

2001 ◽  
Vol 12 (8) ◽  
pp. 465-473 ◽  
Author(s):  
Janos Zempleni ◽  
J Steven Stanley ◽  
Donald M Mock
Keyword(s):  
Mononuclear Cells ◽  
Pantothenic Acid ◽  
National Institutes Of Health ◽  
University Of Arkansas ◽  
Transporter Gene ◽  
Medical Sciences ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells ◽  
Sodium Dependent ◽  
The University

Synthesis of a Rabbit Polyclonal Antibody to the Human Sodium-Dependent Multivitamin Transporter

2002 ◽  
Vol 72 (4) ◽  
pp. 195-198 ◽  
Author(s):  
Jacob Griffin ◽  
Steven Stanley ◽  
Janos Zempleni
Keyword(s):  
Polyclonal Antibody ◽  
Mammalian Cells ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Pantothenic Acid ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells ◽  
Sodium Dependent ◽  
Rabbit Polyclonal Antibody ◽  
And Function

In mammalian cells, biotin is covalently attached to carboxylases and histones and is required for cell proliferation and function. Cellular uptake of biotin (as well as pantothenic acid and lipoic acid) is mediated by the sodium-dependent multivitamin transporter, SMVT. Studies of cellular biotin homeostasis have been hampered by the lack of an antibody to SMVT. Here, we describe the synthesis of a rabbit polyclonal antibody to human SMVT. Using this antibody, SMVT has been identified in human peripheral blood mononuclear cells, Caco-2 cells, and HepG2 cells. Moreover, we observed that cells respond to proliferation with increased synthesis of SMVT.

The expression of ABCG1 transporter gene in peripheral blood mononuclear cells of patients with atherosclerosis

2014 ◽  
Vol 8 (4) ◽  
pp. 337-343 ◽  
Author(s):  
V. V. Miroshnikova ◽  
E. P. Demina ◽  
N. V. Mayorov ◽  
V. V. Davydenko ◽  
P. S. Kurjanov ◽  
...  
Keyword(s):  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Transporter Gene ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells
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