Flow cytometric analyses of the specific activation of peripheral blood mononuclear cells from healthy donors after in vitro stimulation with a fermented mistletoe extract and mistletoe lectins

1998 ◽  
Vol 34 (7) ◽  
pp. 1105-1110 ◽  
Author(s):  
G.M Stein ◽  
P.A Berg
Keyword(s):  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Peripheral Blood Mononuclear ◽  
Mistletoe Extract ◽  
Flow Cytometric ◽  
Healthy Donors ◽  
Blood Mononuclear Cells

scholarly journals Human antibodies isolated from plasma by affinity chromatography increase the coxsackievirus B4-induced synthesis of interferon-α by human peripheral blood mononuclear cells in vitro

2001 ◽  
Vol 82 (8) ◽  
pp. 1899-1907 ◽  
Author(s):  
Wassim Chehadeh ◽  
Ahmed Bouzidi ◽  
Gunar Alm ◽  
Pierre Wattré ◽  
Didier Hober
Keyword(s):  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Interferon Α ◽  
Peripheral Blood Mononuclear ◽  
Coxsackievirus B4 ◽  
Healthy Donors ◽  
Blood Mononuclear Cells

Coxsackievirus B4 (CVB4) can be found in circulating blood of patients; however, the interaction of CVB4 with peripheral blood mononuclear cells (PBMCs) is poorly understood. CVB4 induced low levels of IFN-α synthesis in PBMCs from healthy donors. In contrast, preincubation of infectious CVB4 with plasma from these donors containing anti-CVB4 antibodies strongly enhanced the synthesis of IFN-α. IgG obtained from plasma by chromatography formed immune complexes with CVB4 and increased significantly the CVB4-induced production of IFN-α by PBMCs. These antibodies did not have a neutralizing effect on CVB4 infection of Hep-2 cells. The role of CVB and adenovirus receptor (CAR), FcγRII and FcγRIII in the increased synthesis of IFN-α induced by CVB4 preincubated with IgG was shown by inhibition with specific antibodies. The major interferon-α-producing cells in response to CVB4–IgG complexes were CD14+ cells and monocyte-enriched PBMCs. With the latter, detection of IFN-α by immunostaining was positive whereas in monocyte-depleted PBMCs it was not. This study shows that CVB4-induced synthesis of IFN-α by PBMCs can be enhanced by an antibody-dependent mechanism through interactions between the virus, non-neutralizing antivirus antibodies, FcγRII and III and CAR.

Sign in / Sign up

Export Citation Format

Share Document