Androgen-induced vitellogenin gene expression in primary cultures of rainbow trout hepatocytes

1998 ◽  
Vol 67 (2) ◽  
pp. 133-141 ◽  
Author(s):  
Tsukasa Mori ◽  
Hitoshi Matsumoto ◽  
Hiroaki Yokota
Keyword(s):  
Gene Expression ◽  
Rainbow Trout ◽  
Primary Cultures ◽  
Vitellogenin Gene

Vitellogenin gene expression in male rainbow trout (Salmo gairdneri

1988 ◽  
Vol 71 (3) ◽  
pp. 359-371 ◽  
Author(s):  
Katherine Le Guellec ◽  
Kenneth Lawless ◽  
Yves Valotaire ◽  
Michel Kress ◽  
Martin Tenniswood
Keyword(s):  
Gene Expression ◽  
Rainbow Trout ◽  
Salmo Gairdneri ◽  
Vitellogenin Gene

Influence of xenobiotics on rainbow trout liver estrogen receptor and vitellogenin gene expression

1995 ◽  
Vol 15 (2) ◽  
pp. 143-151 ◽  
Author(s):  
G Flouriot ◽  
F Pakdel ◽  
B Ducouret ◽  
Y Valotaire
Keyword(s):  
Gene Expression ◽  
Estrogen Receptor ◽  
Rainbow Trout ◽  
Expression Vector ◽  
Mrna Accumulation ◽  
Trout Hepatocyte ◽  
Rainbow Trout Hepatocyte ◽  
Vitellogenin Gene ◽  
Rainbow Trout Liver ◽  
Hepatocyte Primary Culture

ABSTRACT Rainbow trout hepatocyte primary culture was used to test the influence of some xenobiotics on the expression of two genes implicated in reproduction, those for the estrogen receptor (ER) and vitellogenin (Vg). We showed that chlordecone, nonylphenol, a polychlorobiphenol (PCB) mixture (Aroclor 1245) and lindane were able to induce ER and Vg mRNA accumulation. Antiestrogens, 4-hydroxytamoxifen and ICI 164,384, prevented the effects of the xenobiotics, indicating that the induction of gene expression is mediated by the ER. Among these four xenobiotics, only chlordecone and nonylphenol were able to displace the binding of [3H]estradiol to ER-enriched COS-1 extracts, and to activate an estrogen-dependent reporter gene (ERE-TK-CAT) cotransfected with an expression vector containing ER cDNA. The results suggest that chlordecone and nonylphenol are direct inducers of rainbow trout ER and Vg gene expression, whereas PCBs and lindane act through their hepatic metabolites. Moreover, pentachlorophenol acts as an antagonist of the induction by estradiol of rainbow trout ER and Vg gene expression.

Vitellogenin gene expression in primary culture of male rainbow trout hepatocytes

1988 ◽  
Vol 70 (2) ◽  
pp. 284-290 ◽  
Author(s):  
Colette Vaillant ◽  
Catherine Le Guellec ◽  
Farzad Pakdel ◽  
Yves Valotaire
Keyword(s):  
Gene Expression ◽  
Rainbow Trout ◽  
Primary Culture ◽  
Vitellogenin Gene

Differences in metallothionein gene expression in primary cultures of rainbow trout hepatocytes and the RTH-149 cell line

1990 ◽  
Vol 1049 (1) ◽  
pp. 78-82 ◽  
Author(s):  
Per-Eric Olsson ◽  
Sven Johan Hyllner ◽  
Muhammad Zafarullah ◽  
Tommy Andersson ◽  
Lashitew Gedamu
Keyword(s):  
Gene Expression ◽  
Rainbow Trout ◽  
Cell Line ◽  
Primary Cultures ◽  
Metallothionein Gene
Sign in / Sign up

Export Citation Format

Share Document