Conservation of feline semen: Part I: Cooling and freezing protocols

G.C Luvoni; E Kalchschmidt; S Leoni; C Ruggiero

doi:10.1016/s1098-612x(03)00029-9

Conservation of feline semen: Part I: Cooling and freezing protocols

Journal of Feline Medicine and Surgery ◽

10.1016/s1098-612x(03)00029-9 ◽

2003 ◽

Vol 5 (4) ◽

pp. 203-208 ◽

Cited By ~ 13

Author(s):

G.C Luvoni ◽

E Kalchschmidt ◽

S Leoni ◽

C Ruggiero

Keyword(s):

Assisted Reproduction ◽

Artificial Insemination ◽

Domestic Cat ◽

Accurate Data ◽

Domestic Cats ◽

Breeding Programs ◽

Long Term Storage ◽

Wild Felids ◽

Term Storage

There has been increased interest recently in the conservation of wild felids and preservation of valuable cat breeds. Assisted reproduction, by means of artificial insemination (AI), is an important tool for developing breeding programs for conservation. Optimal use of AI requires accurate data on semen conservation protocols and its long-term storage/survival. In this paper, semen cooling and freezing processes are described, with special emphasis on the results obtained in experiments performed in the domestic cat. Conception rates after AI in wild and domestic cats are also reported.

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Epididymal tail solid-surface vitrification as an effective method for domestic cat sperm cryobanking

Zygote ◽

10.1017/s096719942100006x ◽

2021 ◽

pp. 1-7

Author(s):

Silmara L.G. Lima ◽

Airton R.B. Soares ◽

Leanne Stalker ◽

Regiane R. Santos ◽

Sheyla F.S. Domingues

Keyword(s):

Solid Surface ◽

Membrane Integrity ◽

Domestic Cat ◽

Sperm Viability ◽

Plasma Membrane Integrity ◽

Long Term Storage ◽

Normal Sperm ◽

Term Storage ◽

Motility Parameters

Summary This study aimed to describe the viability of domestic feline spermatozoa after epididymal tail vitrification. For this, 10 pairs of testis–epididymis complexes were used. The epididymal tails were vitrified using the solid-surface vitrification (SSV) method, in which two vitrification media containing ethylene glycol (EG) 40% or glycerol (GLY) 40% were tested. Vitrification with the presence of EG resulted in better results for all sperm motility parameters (motility, vigour and CASA) compared with GLY (P < 0.05). There were no statistical differences for sperm viability and acrosome integrity, plasma membrane integrity, or overall health of morphologically normal sperm before or after vitrification among experimental groups. In conclusion, epididymal tail vitrification appears to be a suitable method for long-term storage of cat sperm, especially if the procedure is performed with EG as the cryoprotectant.

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Pemanfaatan Kriopreservasi untuk Penyimpanan Bibit Anggrek Jangka Panjang

JURNAL BIOS LOGOS ◽

10.35799/jbl.11.2.2020.28793 ◽

2020 ◽

Vol 10 (2) ◽

pp. 71

Author(s):

Arkan Setiaji ◽

Rr Rifka Annisa

Keyword(s):

Review Article ◽

Breeding Programs ◽

Cold Temperatures ◽

Orchid Species ◽

Long Term Storage ◽

Cryopreservation Techniques ◽

Cryopreservation Protocol ◽

Term Storage

ABSTRAKTanaman anggrek (Orchidaceae) telah menjadi komoditas penting di pasar perdagangan internasional karena keeksotisan dan daya tahan bunganya. Meskipun keragaman anggrek spesies menduduki peringkat kedua setelah Asteraceae dan hibridanya menduduki peringkat pertama, namun anggrek menghadapi ancaman kepunahan dan penurunan kualitas genetik. Program pemuliaan, seperti persilangan dan penyisipan gen unggul, serta perbanyakan in vitro perlu didukung dengan cara memastikan ketersediaan eksplan. Dalam rangka penyimpanan untuk kebutuhan jangka panjang, eksplan dapat disimpan menggunakan teknik kriopreservasi. Review ini bertujuan untuk memberikan gambaran umum terhadap kemajuan penelitian di bidang kriopreservasi, khususnya tanaman anggrek. Melalui program penyimpanan jangka panjang, gen-gen dan eksplan unggulan baik untuk pengembangan anggrek hibrida atau khususnya untuk tujuan konservasi anggrek langka bisa dipastikan ketersediaannya. Dalam artikel review ini telah dibahas berbagai riset tentang teknik kriopreservasi pada biji, protokorm, dan serbuk sari anggrek. Kemajuan pengembangan teknik telah dicapai, namun masih diperlukan banyak modifikasi agar suatu protokol kriopreservasi dapat diterapkan pada semua spesies dan hibrida anggrek.Kata kunci: kriopreservasi; anggrek; suhu dingin; eksplan ABSTRACTOrchid plants (Orchidaceae) have become important commodities in the international trades market because of their exoticism and flower endurance. Although the diversity of orchid species is ranked second after Asteraceae and number of hybrids are ranked first, but orchids face the threat of extinction and genetic degradation. Breeding programs, including such as crossing and insertion of superior genes, and in vitro propagation need to be supported by ensuring the availability of explants. In the context of maintaining storage for long-term needs, explants can be stored using cryopreservation techniques. This review aims to provide an overview of the progress of research in the field of cryopreservation, specifically orchid plants. Through a long-term storage program, superior genes and explants both for the development of hybrid orchids or in particular for the purpose of conservation of rare orchids can be ensured availability. In this review article various researches on cryopreservation techniques in seeds, protocorms, and pollen have been discussed in this article. Advances in technical development have been achieved, but many modifications are still needed so that a cryopreservation protocol can be applied to all orchid species and hybrids.Keywords: cryopreservation; orchids; cold temperatures; explants

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PROFESSOR I. V. SMIRNOV (1911–1993) – ORGANIZER OF THE SYSTEM OF HIGHER PROFESSIONAL EDUCATION AND RESEARCH WORK, FOUNDER OF THE THEORY AND METHODOLOGY OF REPRODUCTIVE BIOTECHNOLOGY

Animal Breeding and Genetics ◽

10.31073/abg.61.01 ◽

2021 ◽

Vol 61 ◽

pp. 5-16

Author(s):

M. V. Gladiy ◽

Yu. P. Polupan ◽

S. I. Kovtun ◽

I. S. Borodai

Keyword(s):

Professional Education ◽

Artificial Insemination ◽

Animal Husbandry ◽

Educational Process ◽

Farm Animals ◽

Long Term Storage ◽

Mammalian Sperm ◽

Higher Professional Education ◽

Term Storage

The purpose of the article is to highlight the main milestones in the life and creative path of the famous scientist in the field of animal husbandry, Doctor of Biological Sciences, Professor I. V. Smirnov, his contribution to the formation of the sectorial experimental work and the spread of higher professional education. Research methods – general scientific (analysis, synthesis, classification), special historical (problem-chronological, comparative-historical, biographical), source studies. The scientific novelty of the article lies in the detailed study of the Terezyno period of I. V. Smirnov's activities, during which the new technological solutions were established and implemented contributing to the development of the theory and methodology of artificial insemination of farm animals. It is proved that on the basis of the Kiev Experimental Station of Animal Husbandry "Terezyno" the scientist implemented the concept of the development of higher professional education, based on the combination of the educational process with long-term industrial practice; laid the foundations for international cooperation in the field of reproduction biotechnology in animal husbandry. The main components of the I. V. Smirnov's creativity are highlighted, namely: methods of sperm long-term storage, technologies and techniques of artificial insemination, biology and physiology of reproduction of farm animals, etc. The researcher developed the theory of anabiosis, the theory of cold sperm shock, established the methods for mammalian sperm diluting and evaluating. He updated research on the use of blood groups for testing the lineage of animals and artificially regulating the sex of the offspring. It is shown that the method of mammalian sperm long-term storage formed the theoretical basis for the reorganization of domestic breeding on the basis of the widespread introduction of artificial insemination, contributed to a fast transformation of the gene pool of farm animals due to the involvement of genetic resources of foreign breeding in the selection process. It has been proved that thanks to the I. V. Smirnov's creative initiatives, the foundations have been laid for the development of a new direction in the science of the reproduction of organic species – reproductive biotechnology of farm animals.

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Modelling of Moisture Movement in Wood during Outdoor Storage

Nonlinear Analysis Modelling and Control ◽

10.15388/na.2001.6.1.15210 ◽

2001 ◽

Vol 6 (2) ◽

pp. 3-14 ◽

Cited By ~ 23

Author(s):

R. Baronas ◽

F. Ivanauskas ◽

I. Juodeikienė ◽

A. Kajalavičius

Keyword(s):

Experimental Data ◽

Finite Difference ◽

Climatic Conditions ◽

Two Dimensional ◽

Moisture Movement ◽

Finite Difference Technique ◽

Long Term Storage ◽

Space Formulation ◽

Term Storage

A model of moisture movement in wood is presented in this paper in a two-dimensional-in-space formulation. The finite-difference technique has been used in order to obtain the solution of the problem. The model was applied to predict the moisture content in sawn boards from pine during long term storage under outdoor climatic conditions. The satisfactory agreement between the numerical solution and experimental data was obtained.

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