Cholesterol sulfate, an activator of protein kinase C mediating squamous cell differentiation: a review

2000 ◽  
Vol 462 (2-3) ◽  
pp. 189-195 ◽  
Author(s):  
Toshio Kuroki ◽  
Tohgo Ikuta ◽  
Mariko Kashiwagi ◽  
Shoko Kawabe ◽  
Motoi Ohba ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Cell Differentiation ◽  
Protein Kinase ◽  
Squamous Cell ◽  
Cholesterol Sulfate ◽  
Kinase C

scholarly journals Induction of Differentiation in Normal Human Keratinocytes by Adenovirus-Mediated Introduction of the η and δ Isoforms of Protein Kinase C

1998 ◽  
Vol 18 (9) ◽  
pp. 5199-5207 ◽  
Author(s):  
Motoi Ohba ◽  
Keiko Ishino ◽  
Mariko Kashiwagi ◽  
Shoko Kawabe ◽  
Kazuhiro Chida ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Cell Differentiation ◽  
Protein Kinase ◽  
Squamous Cell ◽  
Human Keratinocytes ◽  
Kinase C ◽  
Normal Human Keratinocytes ◽  
Transglutaminase 1 ◽  
Normal Human ◽  
Human And Mouse

ABSTRACT Protein kinase C (PKC) plays a crucial role(s) in regulation of growth and differentiation of cells. In the present study, we examined possible roles of the α, δ, η, and ζ isoforms of PKC in squamous differentiation by overexpressing these genes in normal human keratinocytes. Because of the difficulty of introducing foreign genes into keratinocytes, we used an adenovirus vector system, Ax, which allows expression of these genes at a high level in almost all the cells infected for at least 72 h. Increased kinase activity was demonstrated in the cells overexpressing the α, δ, and η isoforms. Overexpression of the η isoform inhibited the growth of keratinocytes of humans and mice in a dose (multiplicity of infection [MOI])-dependent manner, leading to G1 arrest. The η-overexpressing cells became enlarged and flattened, showing squamous cell phenotypes. Expression and activity of transglutaminase 1, a key enzyme of squamous cell differentiation, were induced in the η-overexpressing cells in dose (MOI)- and time-dependent manners. The inhibition of growth and the induction of transglutaminase 1 activity were found only in the cells that express the η isoform endogenously, i.e., in human and mouse keratinocytes but not in human and mouse fibroblasts or COS1 cells. A dominant-negative η isoform counteracted the induction of transglutaminase 1 by differentiation inducers such as a phorbol ester, 1α,25-dihydroxyvitamin D3, and a high concentration of Ca2+. Among the isoforms examined, the δ isoform also inhibited the growth of keratinocytes and induced transglutaminase 1, but the α and ζ isoforms did not. These findings indicate that the η and δ isoforms of PKC are involved crucially in squamous cell differentiation.

Antitumor activity of protein kinase C inhibitors and cisplatin in human head and neck squamous cell carcinoma lines

2002 ◽  
Vol 13 (1) ◽  
pp. 93-100 ◽  
Author(s):  
Thomas K Hoffmann ◽  
Katrin Leenen ◽  
Dieter Hafner ◽  
Vera Balz ◽  
Claus D Gerharz ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Protein Kinase C ◽  
Protein Kinase ◽  
Antitumor Activity ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Squamous Cell ◽  
Human Head ◽  
Protein Kinase C Inhibitors ◽  
Kinase C

Akt, protein kinase C, and mitogen-activated protein kinase phosphorylation status in head and neck squamous cell carcinoma

Head & Neck ◽  
2005 ◽  
Vol 27 (2) ◽  
pp. 130-137 ◽  
Author(s):  
Lara Tosi ◽  
Eliana Rinaldi ◽  
Francesco Carinci ◽  
Antonio Farina ◽  
Antonio Pastore ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Protein Kinase C ◽  
Protein Kinase ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Squamous Cell ◽  
Mitogen Activated Protein Kinase ◽  
Kinase C ◽  
Mitogen Activated Protein ◽  
Kinase Phosphorylation

Protein kinase C isoenzymes in human neuroblasts involvement of PKCε in cell differentiation

FEBS Letters ◽  
1993 ◽  
Vol 322 (2) ◽  
pp. 120-124 ◽  
Author(s):  
M. Ponzoni ◽  
E. Lucarelli ◽  
M.V. Corrias ◽  
P. Cornaglia-Ferraris
Keyword(s):  
Protein Kinase C ◽  
Cell Differentiation ◽  
Protein Kinase ◽  
Kinase C

Protein kinase C mRNA levels and activity in reconstituted normal human epidermis: Relationships to cell differentiation

1992 ◽  
Vol 184 (1) ◽  
pp. 283-291 ◽  
Author(s):  
Roberto Gherzi ◽  
Bianca Sparatore ◽  
Mauro Patrone ◽  
Andrea Sciutto ◽  
Paola Briata
Keyword(s):  
Protein Kinase C ◽  
Cell Differentiation ◽  
Protein Kinase ◽  
Human Epidermis ◽  
Mrna Levels ◽  
Kinase C ◽  
Normal Human

scholarly journals p300/β-Catenin Interactions Regulate Adult Progenitor Cell Differentiation Downstream of WNT5a/Protein Kinase C (PKC)

2016 ◽  
Vol 291 (12) ◽  
pp. 6569-6582 ◽  
Author(s):  
Megan E. Rieger ◽  
Beiyun Zhou ◽  
Nicola Solomon ◽  
Mitsuhiro Sunohara ◽  
Changgong Li ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Cell Differentiation ◽  
Protein Kinase ◽  
Progenitor Cell ◽  
Kinase C

scholarly journals Protein Kinase C ε Is an Endogenous Photosensitizer That Enhances Ultraviolet Radiation-Induced Cutaneous Damage and Development of Squamous Cell Carcinomas1

2004 ◽  
Vol 64 (21) ◽  
pp. 7756-7765 ◽  
Author(s):  
Deric L. Wheeler ◽  
Kaitlin E. Martin ◽  
Kristin J. Ness ◽  
Yafan Li ◽  
Nancy E. Dreckschmidt ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Ultraviolet Radiation ◽  
Squamous Cell ◽  
Kinase C ◽  
Radiation Induced ◽  
Cutaneous Damage
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