Quantifying presynaptic terminals at the light microscope level in intact and deafferented central nervous structures

2002 ◽  
Vol 9 (3) ◽  
pp. 165-172 ◽  
Author(s):  
Inmaculada Gerrikagoitia ◽  
Gontzal Garcı́a del Caño ◽  
Luis Martı́nez-Millán
Keyword(s):  
Light Microscope ◽  
Microscope Level ◽  
Presynaptic Terminals ◽  
Light Microscope Level ◽  
Central Nervous Structures

scholarly journals Distribution of Intravenously Administered Gold Nanoparticles at the Light Microscope Level

2011 ◽  
Vol 25 (S1) ◽  
Author(s):  
Diana C Haines ◽  
Stephen Stern ◽  
Jennifer Hall ◽  
Anil Patri ◽  
Scott McNeil
Keyword(s):  
Gold Nanoparticles ◽  
Light Microscope ◽  
Microscope Level ◽  
Light Microscope Level

Histochemical characteristics of the metacercarial cyst wall of Gynaecotyla adunca

1995 ◽  
Vol 69 (3) ◽  
pp. 223-228
Author(s):  
J. Lee ◽  
M.A. Medlin ◽  
S.T. Dunn
Keyword(s):  
Outer Layer ◽  
Cyst Wall ◽  
Light Microscope ◽  
Structural Modification ◽  
Microscope Level ◽  
Histochemical Analysis ◽  
Light Microscope Level ◽  
Metacercarial Cyst

AbstractThe cyst wall of the metacercaria of Gynaecotyla adunca (Microphallidae: Digenea) was subjected to comprehensive histochemical analysis. At the light microscope level, a uniformly thick, bipartite cyst wall, probably wholly of parasite origin, was evident. Structural modification of the cyst wall to provide an escape aperture was not apparent. The thicker, inner layer was comprised of phospholipid and glyco- and/or mucoproteins, possibly similar in structure to collagen. The outer layer was highly proteinaceous and contained additional amounts of acidic and neutral mucosubstances. The results are discussed in the context of previous observations regarding the excystment requirements of this microphallid species.

Double and triple immunocytochemical labelling at the light microscope level in histopathology

1990 ◽  
Vol 22 (10) ◽  
pp. 530-536 ◽  
Author(s):  
T. Kren�cs ◽  
L. Kren�cs ◽  
B. Boz�ky ◽  
B. Iv�nyi
Keyword(s):  
Light Microscope ◽  
Microscope Level ◽  
Light Microscope Level

Neocallimastix: a comparative morphological study

1991 ◽  
Vol 69 (4) ◽  
pp. 835-843 ◽  
Author(s):  
D. A. Wubah ◽  
M. S. Fuller ◽  
D. E. Akin
Keyword(s):  
Light Microscopy ◽  
Key Words ◽  
Light Microscope ◽  
Morphological Study ◽  
Microscope Level ◽  
Light Microscope Level ◽  
Apical Pore ◽  
Neocallimastix Patriciarum ◽  
Neocallimastix Frontalis ◽  
Mature Thallus

The development from zoospore to a mature thallus in Neocallimastix sp. isolated from a Georgia cow was studied at the light microscope level. The zoospore had 9–14 posteriorly directed flagella, and its shape varied from amoeboid in agar to ovoid in broth. Encysted zoospores developed endogenously into extramatrical ovoid or spherical incipient zoosporangia with extensively branched intramatrical rhizoids that often had constrictions. Sessile mature zoosporangia varied in shape, and zoospores were fully formed within zoosporangia before release through an apical pore. In agar, zoospores encysted close to the parent zoosporangium and developed endogenously into second generation zoosporangia or exogenously into elongate thalli. At maturity, an elongate thallus was made up of a sporangium, a sporangial stalk, a cyst, and branched rhizoids. Elongate thalli were sometimes formed in broth. Melanized resting sporangia were formed on branched thalli in old (> 36 h) cultures. Two isolates of Neocallimastix frontalis from a cow and sheep and Neocallimastix patriciarum were grown under the same conditions as our isolate, and the morphology of zoospore, zoosporangium, and melanized sporangium of the four isolates were compared. In broth, the isolates developed in the same manner and formed elongate thalli and melanized sporangia as described for our isolate. There is insufficient justification, based on morphology alone, for separating the four isolates. The importance of basic light microscopy is discussed. Key words: Neocallimastix, development, morphology.

scholarly journals In situ hybridization at the electron microscope level: hybrid detection by autoradiography and colloidal gold

1982 ◽  
Vol 95 (2) ◽  
pp. 609-618 ◽  
Author(s):  
NJ Hutchison ◽  
PR Langer-Safer ◽  
DC Ward ◽  
BA Hamkalo
Keyword(s):  
In Situ Hybridization ◽  
Electron Microscope ◽  
Satellite Dna ◽  
Light Microscope ◽  
Test System ◽  
Microscope Level ◽  
Metaphase Chromosomes ◽  
Light Microscope Level ◽  
Mouse Satellite Dna

In situ hybridization has become a standard method for localizing DNA or RNA sequences in cytological preparations. We developed two methods to extend this technique to the transmission electron microscope level using mouse satellite DNA hybridization to whole mount metaphase chromosomes as the test system. The first method devised is a direct extension of standard light microscope level using mouse satellite DNA hybridization to whole mount metaphase chromosomes as the test system. The first method devised is a direct extension of standard light microscope in situ hybridization. Radioactively labeled complementary RNA (cRNA) is hybridized to metaphase chromosomes deposited on electron microscope grids and fixed in 70 percent ethanol vapor; hybridixation site are detected by autoradiography. Specific and intense labeling of chromosomal centromeric regions is observed even after relatively short exposure times. Inerphase nuclei present in some of the metaphase chromosome preparations also show defined paatterms of satellite DNA labeling which suggests that satellite-containing regions are associate with each other during interphase. The sensitivity of this method is estimated to at least as good as that at the light microscope level while the resolution is improved at least threefold. The second method, which circumvents the use of autoradiogrphic detection, uses biotin-labeled polynucleotide probes. After hybridization of these probes, either DNA or RNA, to fixed chromosomes on grids, hybrids are detected via reaction is improved at least threefold. The second method, which circumvents the use of autoradiographic detection, uses biotin-labeled polynucleotide probes. After hybridization of these probes, either DNA or RNA, to fixed chromosomes on grids, hybrids are detected via reaction with an antibody against biotin and secondary antibody adsorbed to the surface of over centromeric heterochromatin and along the associated peripheral fibers. Labeling is on average ten times that of background binding. This method is rapid and possesses the potential to allow precise ultrastructual localization of DNA sequences in chromosomes and chromatin.

Mating increases the synthetic activity of the neurosecretory caudodorsal cells of Helisoma duryi (Mollusca: Pulmonata)

1989 ◽  
Vol 67 (10) ◽  
pp. 2363-2367 ◽  
Author(s):  
S. T. Mukai ◽  
A. S. M. Saleuddin
Keyword(s):  
Light Microscope ◽  
Egg Laying ◽  
Microscope Level ◽  
Synthetic Activity ◽  
Leucine Incorporation ◽  
Light Microscope Level ◽  
Cerebral Ganglia ◽  
Helisoma Duryi

In Helisoma duryi, virgin snails lay significantly fewer eggs than mated snails. It is generally accepted that in basommatophoran pulmonates, the neurosecretory caudodorsal cells located in the cerebral ganglia produce a hormone that regulates egg laying. The synthetic activity of the caudodorsal cells in Helisoma has been measured in vitro and in vivo using tritiated leucine. Virgin and castrated snails (reproductively inactive) showed significantly reduced levels of [3H]leucine incorporation compared with first-mated snails (24 and 48 h postmating). This increase in synthetic activity following mating was corroborated by autoradiography at the light microscope level which localized transport of caudodorsal cell neurosecretory proteins to the cerebral commissure, the neurohaemal area. Mating triggers an increase in synthetic activity of the caudodorsal cells.

Intertidal mangrove fungi from north Sumatra

1989 ◽  
Vol 67 (10) ◽  
pp. 3078-3082 ◽  
Author(s):  
Kevin D. Hyde
Keyword(s):  
New Species ◽  
Geographical Distribution ◽  
Light Microscope ◽  
Marine Fungi ◽  
Microscope Level ◽  
Light Microscope Level ◽  
Mangrove Fungi ◽  
First Report ◽  
Mangrove Roots ◽  
North Sumatra

Investigations into the intertidal fungi of Kampong Nelayan Mangrove (Belawan), north Sumatra, yielded 39 species of which one is new to science. Driftwood and mangrove roots and branches were examined. This first report of marine fungi from north Sumatra extends our knowledge of their ecology and geographical distribution. The new species is described and illustrated at the light microscope level.

The structure and development of the pigmented retinal clone

1975 ◽  
Vol 53 (5) ◽  
pp. 560-570 ◽  
Author(s):  
Bruce J. Crawford
Keyword(s):  
Epithelial Cells ◽  
Light Microscope ◽  
Microscope Level ◽  
Light Microscope Level ◽  
Differentiated Cells ◽  
Cytological Changes ◽  
Pass Through ◽  
Flocculent Material ◽  
Clone Cells

Cytological changes during growth and differentiation of chick pigmented epithelial cells in clonal culture are described at the light microscope level. Initially the cells are squamous and unpigmented. By 3 weeks, cells in the center of the colonies are highly differentiated, i.e. they are polygonal in shape and densely pigmented. During differentiation, cells in the center of the colony pass through a series of morphological stages comparable to those seen from the edge to the center of a 3-week-old clone. Cells in the inner two zones of a differentiated colony resemble stages found during differentiation in vivo. Those in the outer two zones appear to have no morphological counterparts in the intact animal.Extracellular materials are abundant in the stratified zone of the colony. These exhibit staining properties similar to those of acid mucopolysaccharides found in the developing retina. Vacuoles containing a flocculent material appear within the differentiated cells of colonies older than 2.5 weeks. Later, some of these vacuoles disappear and flocculent material is found between the cells and the plate. The possible significance of these observations is discussed.

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