P.327 Detection and characterization of hepatitis A virus RNA in fulminant hepatic failure in children in Argentina

Aim: To assess fulminant hepatic failure (FHF) in children and its interrelating factors. Methods and materials: A retrospective review study of 24 patients less than 17 years old who presented to the National Liver Institute with FHF over a period of two years was done. FHF was defined as the presence of acute liver failure with or without encephalopathy without pre-existing liver disease, within 8 weeks of the onset of clinical liver disease. Hepatitis A virus (HAV) IgM antibodies, Hepatitis B virus surface antigen (HBsAg), IgM-anti Hepatitis B core antigen (HBc), Hepatitis E virus (HEV) IgM and Hepatitis C virus (HCV) RNA in nested PCR were done in all patients. Special investigations like alpha fetoprotein, serum copper, blood culture, drug levels and other metabolic studies were carried out whenever indicated. Detailed clinical evaluations and routine hepatic laboratory profile were done. Results: Acute hepatitis-A virus was the commonest cause. Severe coma was significantly present among non-survivals and no ascites. Patients who survived had got significantly lower level of prothrombin time, they also had significantly lower rate of descent of prothrombin time. Conclusion: Hepatitis A virus was the commonest etiology of FHF. The peak level of total serum bilirubin, the rate of change of the prothrombin time/day and ammonia level were significant predictors of mortality.

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S2471 A Fatal Case of Fulminant Hepatic Failure Associated With Hepatitis A Virus

The American Journal of Gastroenterology ◽

10.14309/01.ajg.0000711932.01386.86 ◽

2020 ◽

Vol 115 (1) ◽

pp. S1307-S1308

Author(s):

Fathima K. Suhail ◽

Moeed R. Chohan ◽

Abdul Qadir Bhutta ◽

Gowthami Kanagalingam ◽

Nuri Ozden

Keyword(s):

Fulminant Hepatic Failure ◽

Hepatic Failure ◽

Hepatitis A ◽

Hepatitis A Virus ◽

Fatal Case

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In vitro characterization of an internal ribosomal entry site (IRES) present within the 5' nontranslated region of hepatitis A virus RNA: comparison with the IRES of encephalomyocarditis virus.

Journal of Virology ◽

10.1128/jvi.68.2.1066-1074.1994 ◽

1994 ◽

Vol 68 (2) ◽

pp. 1066-1074 ◽

Cited By ~ 99

Author(s):

E A Brown ◽

A J Zajac ◽

S M Lemon

Keyword(s):

Hepatitis A ◽

Hepatitis A Virus ◽

Internal Ribosomal Entry Site ◽

Encephalomyocarditis Virus ◽

Entry Site ◽

In Vitro Characterization ◽

Virus Rna ◽

Nontranslated Region

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Detection of hepatitis A virus RNA from children patients with acute and fulminant hepatitis of unknown etiology in Vietnam: Genomic characterization of Vietnamese HAV strain

Pediatrics International ◽

10.1111/j.1442-200x.2008.02626.x ◽

2008 ◽

Vol 50 (5) ◽

pp. 624-627 ◽

Cited By ~ 4

Author(s):

Phuc Le Hoang ◽

Khanh Huu Trong ◽

Tung Thanh Tran ◽

Tran Thien Tuan Huy ◽

Kenji Abe

Keyword(s):

Fulminant Hepatitis ◽

Hepatitis A ◽

Hepatitis A Virus ◽

Unknown Etiology ◽

Virus Rna ◽

Genomic Characterization

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Study of acute viral hepatitis with special reference to fulminant hepatic failure

International Journal of Contemporary Pediatrics ◽

10.18203/2349-3291.ijcp20182559 ◽

2018 ◽

Vol 5 (4) ◽

pp. 1533

Author(s):

Rajesh N. Pankhaniya ◽

Yogesh N. Parikh ◽

Dhanya Soodhana Mohan

Keyword(s):

Hepatic Encephalopathy ◽

Viral Hepatitis ◽

Fulminant Hepatic Failure ◽

Hepatic Failure ◽

Hepatitis E Virus ◽

Hepatitis A ◽

Acute Viral Hepatitis ◽

Hepatitis A Virus ◽

Hepatitis E ◽

Age Group

Background: Fulminant hepatic failure is a complication of viral hepatitis and is one of the leading causes of death in hospitalized children with viral hepatitis in India.Methods: All the patients suspected to have acute viral hepatitis and/or fulminant hepatic failures with hepatic encephalopathies were included in the study and the diagnosis was made on the basis of history, clinical examination including a detailed neurological examination. Triage scoring, Glasgow coma scale, encephalopathy grading were recorded at the time of admission and necessary investigations were carried out.Results: The occurrence of acute viral hepatitis and fulminant hepatic failure was 200 (2.004%) and 40 (0.401%) respectively during study period. Highest occurrence of 35.50% in the age group of 4-6 years, yellowish discoloration of the eyes or the urine was the most common presenting complaint. Only 45 % of the children who had fulminant hepatic failure survived and mortality was found to be 100% in those children who had grade 4 hepatic encephalopathy. Mortality was higher in those children who had duration of more than 10 days between the onset of jaundice and encephalopathy. Mortality was higher in those with prothrombin time between 41 to 50 seconds and with serum ammonia level more than 200 mmol/l. Hepatitis A virus was the most common cause of fulminant hepatic failure and Hepatitis E virus with hepatic encephalopathy was associated with 100 % mortality.Conclusions: Hepatitis A infection though associated with a good prognosis, younger age group (< 6 years) and co infection with Hepatitis E virus have a poor prognosis. Good hygiene practices and early immunization could be a step towards the prevention of Hepatitis A infection.

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Application of solid-phase immune electron microscopy to study physical and stability characteristics of enterically transmitted non-a, non-b hepatitis virus

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100102481 ◽

1988 ◽

Vol 46 ◽

pp. 80-81

Author(s):

Charles D. Humphrey ◽

E. H. Cook ◽

Karen A. McCaustland ◽

Daniel W. Bradley

Keyword(s):

Electron Microscopy ◽

Hepatitis A ◽

Hepatitis A Virus ◽

Solid Phase ◽

Etiologic Agent ◽

World Health ◽

Health Concern ◽

Morphological Identification ◽

Immune Electron Microscopy

Enterically transmitted non-A, non-B hepatitis (ET-NANBH) is a type of hepatitis which is increasingly becoming a significant world health concern. As with hepatitis A virus (HAV), spread is by the fecal-oral mode of transmission. Until recently, the etiologic agent had not been isolated and identified. We have succeeded in the isolation and preliminary characterization of this virus and demonstrating that this agent can cause hepatic disease and seroconversion in experimental primates. Our characterization of this virus was facilitated by immune (IEM) and solid phase immune electron microscopic (SPIEM) methodologies.Many immune electron microscopy methodologies have been used for morphological identification and characterization of viruses. We have previously reported a highly effective solid phase immune electron microscopy procedure which facilitated identification of hepatitis A virus (HAV) in crude cell culture extracts. More recently we have reported utilization of the method for identification of an etiologic agent responsible for (ET-NANBH).

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