619 ACTIVATED FIBROBLAST GROWTH FACTOR-INDUCIBLE 14 (FN14) PROMOTES INVASION, MIGRATION AND PROLIFERATION OF ANDROGEN INDEPENDENT PROSTATE CANCER CELLS THROUGH MATRIX METALLOPROTEINASE 9 AND CORRELATES WITH POOR TREATMENT OUTCOME

2011 ◽  
Vol 10 (2) ◽  
pp. 201
Author(s):  
S. Narita ◽  
M. Mingguo ◽  
N. Tsuchiya ◽  
K. Numakura ◽  
T. Obara ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Treatment Outcome ◽  
Growth Factor ◽  
Matrix Metalloproteinase ◽  
Fibroblast Growth ◽  
Prostate Cancer Cells ◽  
Poor Treatment ◽  
Poor Treatment Outcome ◽  
Metalloproteinase 9 ◽  
Androgen Independent

scholarly journals Overexpression of Fn14 promotes androgen-independent prostate cancer progression through MMP-9 and correlates with poor treatment outcome

2011 ◽  
Vol 32 (11) ◽  
pp. 1589-1596 ◽  
Author(s):  
Mingguo Huang ◽  
Shintaro Narita ◽  
Norihiko Tsuchiya ◽  
Zhiyong Ma ◽  
Kazuyuki Numakura ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Treatment Outcome ◽  
Cancer Progression ◽  
Prostate Cancer Progression ◽  
Poor Treatment ◽  
Poor Treatment Outcome ◽  
Androgen Independent

scholarly journals Effect of Caffeic Acid Phenethyl Ester Provision on Fibroblast Growth Factor-2, Matrix Metalloproteinase-9 Expression, Osteoclast and Osteoblast Numbers during Experimental Tooth Movement in Wistar Rats (Rattus norvegicus)

2021 ◽  
Author(s):  
Ida Bagus Narmada ◽  
Paristyawati Dwi Putri ◽  
Lucky Lucynda ◽  
Ari Triwardhani ◽  
I Gusti Aju Wahju Ardani ◽  
...  
Keyword(s):  
Growth Factor ◽  
Matrix Metalloproteinase ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Tooth Movement ◽  
Caffeic Acid Phenethyl Ester ◽  
Fibroblast Growth Factor 2 ◽  
Fibroblast Growth ◽  
Male Wistar Rats ◽  
Metalloproteinase 9

Abstract Objectives To investigate the effect of caffeic acid phenethyl ester (CAPE) provision on matrix metalloproteinase-9 (MMP-9), fibroblast growth factor-2 (FGF-2) expression, osteoclast and osteoblast numbers during experimental orthodontic tooth movement (OTM) in male Wistar rats (Rattus norvegicus). Materials and Methods Forty-eight healthy male Wistar rats (R. norvegicus), 16 to 20 weeks old with 200 to 250 g body weight (bw) were divided into several groups as follows: K1: OTM for 3 days; K2: OTM for 7 days; K3: OTM for 14 days; KP1: OTM and CAPE for 3 days; KP2: OTM and CAPE for 7 days; and KP3: OTM and CAPE for 14 days. A nickel titanium closed coil spring 8.0 mm long with 10 g/mm2 was installed between the upper left first molar and upper central incisor to move molar mesially. CAPE provision with a dose of 20 mg/kg bw of animal studies was done per orally. Immunohistochemistry was done to examine MMP-9 expression and osteoclast number in compression side as well as FGF-2 expression and osteoblast number in tensile side of the OTM. Statistical Analysis One-way analysis of variance test and Tukey’s honest significant difference test were performed to determine the difference between the groups (p < 0.05). Results MMP-9 expression and osteoclast numbers in the compression side were significantly different between the groups. Similarly, FGF-2 expression and osteoclast numbers in the tensile side were significantly different between the groups. Conclusions CAPE provision during OTM increases the number of osteoblasts and the FGF-2 expression significantly in the tensile side. Osteoclast numbers and MMP-9 expression significantly decrease in the compression side.

scholarly journals Epidermal growth factor-induced neuroendocrine differentiation and apoptotic resistance of androgen-independent human prostate cancer cells

2006 ◽  
Vol 13 (1) ◽  
pp. 181-195 ◽  
Author(s):  
S Humez ◽  
M Monet ◽  
G Legrand ◽  
G Lepage ◽  
P Delcourt ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Cancer Cells ◽  
Neuroendocrine Differentiation ◽  
Calcium Entry ◽  
Calcium Overload ◽  
Prostate Cancer Cells ◽  
Epidermal Growth ◽  
Androgen Independent

Neuroendocrine differentiation (NED) has been implicated in prostate cancer progression and hormone-therapy failure. Neuroendocrine cells are non-proliferating and escape apoptotic cell death, although their origin and the causes of their apoptotic resistance have as yet been poorly elucidated. This study demonstrates a new mechanism involved in controlling NED. We report that epidermal growth factor (5–50 ng/ml) promotes neuroendocrine-like differentiation of androgen-independent DU145 prostate cancer cells. This differentiation is associated with an increase in the expression of Neuron Specific Enolase (NSE) and a reduction in cell proliferation and is blocked by inhibiting tyrosine kinase activity with genistein and with compound 56 (C56). An increase in the cAMP level, using dibutryl cAMP (db-cAMP) (1 mM) and isobutylmethylxanthine (100 μM), does not promote NED by itself, but does increase the effect of EGF on NED. In addition, EGF-induced NED protects cells from apoptosis induced with thapsigargin (1 μM) by reducing the thapsigargin-induced cytosolic calcium overload. In order to describe how EGF-induced NED protects cells against thapigargin-induced calcium overload we investigated the spatiotemporal calcium signalling linked to apoptosis. By using thapsigargin in various conditions on DU145 cells and using micro-fluorimetric calcium measurements, we show that depletion of intracellular calcium store induces apoptosis and that the amplitude and duration of the capacitive calcium entry are two apoptosis-modulating parameters. We show that protection against thapsigargin-induced apoptosis conferred by NED is achieved by reducing the amount and the speed of calcium that can be released from calcium pools, as well as modulating the amplitude of the subsequent calcium entry.

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