Free radical scavenging activity of Castanopsis indica in mediating hepatoprotective activity of carbon tetrachloride intoxicated rats

2012 ◽  
Vol 2 (1) ◽  
pp. S243-S251 ◽  
Author(s):  
Narayan Dolai ◽  
Indrajit Karmakar ◽  
RB Suresh Kumar ◽  
Biswakanth Kar ◽  
Asis Bala ◽  
...  
Keyword(s):  
Free Radical ◽  
Carbon Tetrachloride ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Hepatoprotective Activity ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Castanopsis Indica

scholarly journals Antimicrobial, Cytotoxic, Thrombolytic and Antioxidant Activities of Syzygium fruticosum (Roxb.) DC.

2015 ◽  
Vol 17 (1) ◽  
pp. 51-54
Author(s):  
Somaia Haque Chadni ◽  
Abdullah Al Hasan ◽  
ATM Zafrul Azam
Keyword(s):  
Free Radical ◽  
Carbon Tetrachloride ◽  
Soluble Fraction ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Zone Of Inhibition ◽  
Aqueous Fraction

The crude methanol extracts of the bark and leaves of Syzygium fruticosum (Roxb.) DC. were partitioned with petroleum ether, carbon tetrachloride, chloroform and water for biological investigation. In the brine shrimp lethality bioassay, carbon tetrachloride soluble fraction of leaf extract (LCTF) showed significant lethality having the LC50 value 0.65 ?g/ml. In free radical scavenging activity screening (DPPH assay), chloroform fraction of methanolic extract of bark of S. fruticosum showed the highest free radical scavenging activity with IC50 value 20.01 ?g/ml. In the microbiological investigation, only chloroform soluble fraction of bark (BCF) and aqueous fraction of bark (BAF) showed mild antimicrobial activity with zone of inhibition ranging from 7 to 14 mm as compared to standard ciprofloxacin (zone of inhibition of 50 mm). In the study for thrombolytic property, different extracts of S. fruticosum revealed varying degrees of thrombolytic activity ranging from 33.46% to 62.51%. DOI: http://dx.doi.org/10.3329/bpj.v17i1.22315 Bangladesh Pharmaceutical Journal 17(1): 51-54, 2014

scholarly journals CHARACTERIZATION, IN VITRO ANTIOXIDANT AND HEPATOPROTECTIVE ACTIVITY OF FUNGAL ENDOPHYTIC EXTRACTS OF ANDROGRAPHIS PANICULATA LEAVES IN CCL4 INDUCED HEPATOTOXICITY

2019 ◽  
Vol 11 (1) ◽  
pp. 44 ◽  
Author(s):  
Smita K. Puri ◽  
Prasanna V. Habbu ◽  
Preeti V. Kulkarni ◽  
Venkatrao H. Kulkarni
Keyword(s):  
Free Radical ◽  
Endophytic Fungi ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Hepatoprotective Activity ◽  
Free Radical Scavenging ◽  
Andrographis Paniculata ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity

Objective: To isolate and characterize the endophytic fungi from the leaves of Andrographis paniculata for free radical scavenging antioxidant and hepatoprotective activity against CCl4 induced hepatotoxicityMethods: Two fungal endophytes, APLF-3 (Andrographis paniculata leaf fungi-3) and APLF-4 (Andrographis paniculata leaf fungi-4) were isolated from leaves of Andrographis paniculata to get chloroform (A3C, A4C), ethyl acetate (A3EA, A4EA) and n butanol (A3nB, A4nB) extracts. rDNA sequencing by PCR technique was carried out for identification of APLF-3 and APLF-4. All the APLF-3 and APLF-4 extracts were assayed for in vitro free radical scavenging activity against 2, 2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl radical and reducing power. Then, A4EA and A4nB were screened for hepatoprotective activity against CCl4 induced hepatotoxicity at 50 mg/kg and100 mg/kg doses.Results: The endophytic fungi, APLF-3 and APLF-4, were identified as Phyllosticta sp. ZLY-2010 isolate M13 and Aspergillus tubingensis strain Cs/7/2 respectively based on their morphological and molecular characterization. A4EA and A4nB showed significant in vitro free radical scavenging activity as compared to other extracts. A4EA and A4nB (50 mg/kg and100 mg/kg) reversed the increased serum biochemical parameters as compared to CCl4 treated group (p<0.001). A4EA and A4nB (100 mg/kg p. o) also restored the LPO, SOD and CAT levels.Conclusion: These findings suggested that the extracts (A4EA and A4nB) obtained from endophytic fungi APLF-4 contributed towards hepatoprotective activity.

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