Imaging Mechanisms of Drug Resistance in Experimental Models of Epilepsy

The aimis to develop an antiepileptic drug based on polymer nanoparticles with 2-ethyl-6-methyl-3-oxypyridine succinate to facilitate the drug transport through the blood-brain barrier.Materials and methods.The nano-drug was created using the biologically active substance 2-ethyl-6-methyl-3-hydroxypyridine succinate and polybutyl cyanoacrylate (PBCA) nanoparticles. The advantages of this nano-form over the active ingredient of the same drug were studied using experimental models: the maximum electroshock test (MES), the antagonism test with corazol, models with a cobaltinduced epileptic focus and secondary generalized convulsions, and models of status epilepticus.Results.The antiseizure effects of the nanoform on the experimental models of epilepsy are identified.Conclusion.The nano-drug reduces the number of secondary generalized clonic-tonic seizures by 7.8 times; it also reduces 10-fold the animal mortality and diminishes the seizure manifestations that occur in the interictal period of the epileptic status.

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Gaseous neurotransmitter nitric oxide: Its role in experimental models of epilepsy

Archives of Biological Sciences ◽

10.2298/abs1203207h ◽

2012 ◽

Vol 64 (3) ◽

pp. 1207-1216 ◽

Cited By ~ 6

Author(s):

D. Hrncic ◽

Aleksandra Rasic-Markovic ◽

Jelica Bjekic-Macut ◽

Veselinka Susic ◽

D. Mladenovic ◽

...

Keyword(s):

Nitric Oxide ◽

Experimental Studies ◽

Experimental Models ◽

Initiation And Propagation ◽

Organ Systems ◽

The Central Nervous System ◽

Animal Models Of Epilepsy ◽

Almost All ◽

Gaseous Neurotransmitter ◽

Models Of Epilepsy

Epilepsy is one of the leading neurological disorders and affects 1-2% of the world?s population. Generally, it is a result of an imbalance between excitatory and inhibitory phenomena in the central nervous system (CNS), but the mechanisms of its initiation and propagation still require further investigations. Experimental models represent one of the most powerful tools to better understand the mechanisms of epileptogenesis. Nitric oxide (NO) is gaseous molecule with pleiotropic physiological and pathological effects in almost all organ systems and intriguing biological relevance, especially in the CNS where it acts as a gaseous neurotransmitter. The role of NO in the generation of epilepsy is highly contradictory, since there is evidence of its anticonvulsive, as well as proconvulsive properties. Therefore, we will discuss in this review the involvement of NO-mediated signaling pathways in the mechanisms of epileptogenesis, taking into account the findings revealed in experimental studies on animal models of epilepsy.

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