scholarly journals Effects of Nori- and Wakame-enriched meats with or without supplementary cholesterol on arylesterase activity, lipaemia and lipoproteinaemia in growing Wistar rats

2011 ◽  
Vol 106 (10) ◽  
pp. 1476-1486 ◽  
Author(s):  
Raúl Olivero-David ◽  
Adriana Schultz-Moreira ◽  
Miguel Vázquez-Velasco ◽  
Laura González-Torres ◽  
Sara Bastida ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Plasma Cholesterol ◽  
Dietary Cholesterol ◽  
Lipoprotein Metabolism ◽  
Density Lipoprotein ◽  
Control Group ◽  
Vldl Cholesterol ◽  
Freeze Dried ◽  
Cholesterol Supplementation ◽  
Two Parameters

Some seaweeds exert antioxidant and hypocholesterolaemic properties. The effects of diets including restructured meats (RM) containing Wakame (W) or Nori (N) algae on arylesterase (AE) activity and lipoprotein concentration and composition were tested. In the present study, six groups of ten male growing Wistar rats each were fed a mix of 85 % AIN-93M diet and 15 % freeze-dried RM for 35 d. The control group (C) consumed control RM, the W and N groups consumed RM with 5 % W and 5 % N, respectively. The cholesterol-enriched C (CC), W (CW) and N (CN) groups consumed their corresponding basal diets with supplementary cholesterol (2·43 %) and cholic acid (0·49 %). Cholesterol in the diet induced lower (P < 0·001) growth ratios. Both W and N diets significantly increased AE activity. VLDL-cholesterol values were lower in N rats than in W rats. AE activity increased (P < 0·001) in CC and CW rats but not in CN rats compared with their corresponding counterparts. AE was lower (P < 0·05) in the CN group than in the CC and CW groups. The CN diet partially blocked (P < 0·001) the hypercholesterolaemic induction observed in CC and CW diets and reduced TAG levels (at least P < 0·05) with respect to those of CC rats. Although dietary cholesterol supplementation increased total cholesterol, VLDL-cholesterol and (intermediate-density lipoprotein+LDL)-cholesterol (all P < 0·001) in all rats, the CN diet moderately improved the lipoprotein profile of hypercholesterolaemic rats. Changes in AE activity and plasma cholesterol in CN rats but not in CW rats suggest a possible relationship between the two parameters. It is concluded that inclusion of RM enriched with N may be used in hypercholesterolaemic diets to improve lipoprotein metabolism.

scholarly journals Effect of seaweed and cholesterol-enriched diets on postprandial lipoproteinaemia in rats

2009 ◽  
Vol 102 (12) ◽  
pp. 1728-1739 ◽  
Author(s):  
Aránzazu Bocanegra ◽  
Sara Bastida ◽  
Juana Benedí ◽  
Meritxell Nus ◽  
Jose M. Sánchez-Montero ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Plasma Cholesterol ◽  
Dietary Treatment ◽  
Wheat Starch ◽  
The Other ◽  
Lipoprotein Profile ◽  
Postprandial Lipaemia ◽  
Freeze Dried ◽  
Male Wistar Rats ◽  
Cholesterol Supplementation

High postprandial lipaemia increases cardiovascular risk. Algae consumption may affect postprandial lipoproteinaemia. The effects of dietary alga and cholesterol supplementation on postprandial lipaemia and lipoproteinaemia and arylesterase (AE) activity in growing male Wistar rats were tested in the present study. Six groups of ten rats were fed a casein-based diet for 3 weeks. Three of the diets contained 2·4 % cholesterol-raising agent (Chol), while the other three did not (NChol). Seven percentage of the control diets (NChol-C and Chol-C) consisted of a cellulose–wheat starch mix (35:65), while the Nori alga diets (NChol-N and Chol-N) and Konbu diets (NChol-K and Chol-K) contained 7 % of each respective freeze-dried alga. Postprandial plasma was obtained after a 3 h diet withdrawal. Supplementary cholesterol and alga type significantly affected (at least P < 0·05) the cholesterol, TAG, phospholipid and protein contents of the various lipoprotein fractions. AE enzyme activity increased (P < 0·05) in NChol rats given Nori and Konbu diets. NChol-K, but not NChol-N, rats displayed higher (P < 0·05) plasma cholesterol, TAG and phospholipid levels than NChol-C animals. NChol-K rats presented higher TAG, phospholipid, protein and lipoprotein mass values than their NChol-C counterparts. Inclusion of algae in Chol diets decreased (P < 0·001) the postprandial hypertriacylglycerolaemia. The Chol-N diet affected most lipoprotein fraction contents. Chol-N rats had lower postprandial cholesterolaemia and a better lipoprotein profile (fewer LDL and a tendency toward more HDL and fewer cholesterol-enriched VLDL) than Chol-K rats, suggesting that Nori is the alga of choice in dietary treatment of hypercholesterolaemia.

scholarly journals A Nori but not a Konbu, dietary supplement decreases the cholesterolaemia, liver fat infiltration and mineral bioavailability in hypercholesterolaemic growing Wistar rats

2008 ◽  
Vol 99 (2) ◽  
pp. 272-280 ◽  
Author(s):  
Arancha Bocanegra ◽  
Ana Nieto ◽  
Sara Bastida ◽  
Juana Benedí ◽  
Francisco J. Sánchez-Muniz
Keyword(s):  
Dietary Supplement ◽  
Wistar Rats ◽  
Body Weight Gain ◽  
Plasma Cholesterol ◽  
Dietary Cholesterol ◽  
Liver Fat ◽  
Control Group ◽  
Efficiency Ratio ◽  
Fat Infiltration ◽  
Apparent Absorption

The nutritional consequences of algae consumption in young populations consuming hypercholesterolaemic diets have hardly been investigated. This study tests the effect of algae supplementation of cholesterol-enriched balanced diets on growth, dietary efficiency ratio, mineral intake and absorption, organ weight and structure and cholesterolaemia in growing Wistar rats. Three groups of ten rats each were fed for 3 weeks with experimental diets containing 93 % casein-soyabean oil base with 2·4 % cholesterol-raising agent and 7 % supplement. The control group received cellulose (35 %), group 2 consumed Nori (33·8 % fibre) and group 3 consumed Konbu (36·1 % fibre). Food intake and body weight gain were not significantly affected. Algae groups presented significantly higher dietary efficiency ratio values than control rats. Apparent absorption of several minerals appeared significantly affected, mainly in Nori-fed rats, with a significant decrease in the ratio of Zn and Cu intakes and apparent absorption. Nori diet significantly decreased plasma cholesterol. Algae supplement did not significantly affect organ size and structure. Control and Konbu rats showed severe liver fat infiltration, while Nori rats exhibited a significantly lower degree of lipid-like hepatocyte vacuolization but light to moderate leukocyte infiltration. Light to moderate scaling off of the epithelium and moderate submucosa oedema was observed in all groups. Although long-term studies are needed to check the possible extrapolation of these data to human subjects, it can be concluded that a Nori, but not a Konbu, dietary supplement reverses the negative effect of dietary cholesterol intake and also appears to be related to mineral availability in growing subjects.

scholarly journals Flaxseed, olive and fish oil influence plasmatic lipids, lymphocyte migration and morphometry of the intestinal of Wistar rats

2010 ◽  
Vol 25 (3) ◽  
pp. 275-280 ◽  
Author(s):  
Damiana Diniz Rosa ◽  
Regiane Lopes de Sales ◽  
Luis Fernando de Sousa Moraes ◽  
Fabíola Cesário Lourenço ◽  
Clóvis Andrade Neves ◽  
...  
Keyword(s):  
Fish Oil ◽  
Intestinal Mucosa ◽  
Wistar Rats ◽  
Density Lipoprotein ◽  
Parameter Analysis ◽  
Male Rats ◽  
Histological Evaluation ◽  
Control Group ◽  
Soy Oil ◽  
Lymphocyte Migration

PURPOSE: Evaluate the effect of flaxseed, olive and fish oil on the lipid profile, preservation of villosities and lymphocyte migration in the intestinal mucosa of Wistar rats. METHODS: Thirty Wistar male rats were divided into four groups, which received the AIN-93M diet, with changes only to their lipid source: flaxseed, olive, fish, and soy oil (control group). The serum was separated for the biochemical parameter analysis. A histological evaluation was performed in the ileal portion. RESULTS: The group which was fed fish oil presented lower values when compared to the other treatments for Total Cholesterol, High-density Lipoprotein Cholesterol and Triacylglycerol (p<0.05). The animals treated with fish and olive oils presented better intestinal villosities preservation. Less deposition of lymphocytes was observed in the flaxseed group (p<0.001). CONCLUSIONS: This study demonstrated that flaxseed, olive and fish oils present different responses than soy oil for the intestinal mucosa preservation and lymphocyte proliferation in Wistar rats.

scholarly journals RESVERATROL INCLUSION COMPLEX WITH β-CYCLODEXTRIN (RCD): CHARACTERIZATION AND EVALUATION OF TOXICITY IN WISTAR RATS

2016 ◽  
Vol 9 (1) ◽  
pp. 27 ◽  
Author(s):  
V. S. K. Nishihira ◽  
N. J. Mezzomo ◽  
M. D. Baldissera ◽  
R. A. Vaucher ◽  
C. G. Pinto ◽  
...  
Keyword(s):  
Inclusion Complex ◽  
Oral Administration ◽  
Biochemical Parameters ◽  
Wistar Rats ◽  
Metabolic Diseases ◽  
Hematological Parameters ◽  
Density Lipoprotein ◽  
Blood Parameters ◽  
Control Group ◽  
Scanning Calorimetry

<p class="RSCB01ARTAbstract"><strong>Objective</strong>:<strong> </strong>The aim of this study was to characterise the resveratrol inclusion complex with β-cyclodextrin (RCD) and evaluate their toxicity in wistar rats.</p><p class="RSCB01ARTAbstract"><strong>Methods: </strong>The RCD were prepared in ultra-turrax. For characterization of the RCD were used: Fourier transform infra-red Spectroscopy, Nuclear Magnetic Resonance (NMR), Differential Scanning Calorimetry (DSC) and X-ray powder diffraction. The RCD and others 4 treatments were performed by the chronic oral administration in 35 rats during 60 ds. After the treatments they were euthanized and the serum blood were collected to analyzed some hemogram and biochemical parameters including aspartyl aminotransferase (AST); alanine aminotransferase (AST); phosphatase alkaline (ALP); total bilirubin (TB); direct bilirubin (DB); total protein (TP); total cholesterol (TC), triacylglycerol (TAG), very low-density lipoprotein (VLDL), high-density lipoprotein (HDL), calcium, iron and phosphate using fully automated biochemistry analyzer.</p><p class="RSCB01ARTAbstract"><strong>Results: </strong>The characterization results indicated a successful formation of the RCD. All hematological parameters analysed were within the normal values in all the groups. Furthermore, the hemogram and biochemical parameters were significantly (P&gt;0.05) similar to the control group.</p><p class="RSCB01ARTAbstract"><strong>Conclusion: </strong>The daily oral administration during 60 d of RCD are not harmful on blood parameters of Wistar rats. Thus, RCD can be used safely for treatment of some metabolic diseases.</p>

scholarly journals PENGARUH PEMBERIAN QUERCETIN TERHADAP KADAR LEPTIN SERUM TIKUS WISTAR YANG DIBERI DIET TINGGI LEMAK

2012 ◽  
Vol 6 (2) ◽  
Author(s):  
Sudiarto . ◽  
Sri Hidayati Suprihatin
Keyword(s):  
Metabolic Syndrome ◽  
Wistar Rats ◽  
Block Design ◽  
Density Lipoprotein ◽  
Control Group ◽  
Diet Induced Obesity ◽  
Post Test ◽  
Ultimate Cause ◽  
Chronical Disease ◽  
High Level

Obesity, one of the degenerative diseases, is a risk factor for chronical disease and an ultimate cause of metabolic syndrome which ismarked by the raising level of leptin on blood (hyperleptinemia). Metabolic syndrome is marked by the size of the waist is more than 40 inchfor man or 35 inch for woman, hypertension, hyperglycemia, high level of triglyceride, and the level of HDL (high-density lipoprotein) is low.This research is aimed to know the giving of quercetin (which has high anti oxide activities) on the declining level of leptin to the giving of DIO(Diet Induced Obesity). An experimental study using Post Test Control Group was done to the experimental wistar rats. Samples werechosen by using randomized complete block design. Rats were treated for 8 weeks. The dose of given quercetin were 2 mg/kgBB/day, 10mg/kgBB/day, and 50 mg/kgBB/day during the last 8 weeks of research. The variable measured in this research is the level of leptin onblood. Based on the experiment, quercetin is proven that gives influence to the level of leptin on obesity wistar rats’ serum compare to the givenlevel of leptin on controlled rats. There is different average on the level of leptin on wistar rats’ serum which being obesity on each grouptreatment. The giving of quercetin have significant correlation (p<0.05) with the level of leptin on wistar rats’ serum which being obesity (r = -0.704, p = 0.001). The conclusion from this research is the giving of quercetin to obesity rats for 8 weeks can decline the level of leptin onblood.Keywords: quercetin, leptin, high fat diet

scholarly journals Black, pinto and white beans lower hepatic lipids in hamsters fed high fat diets by excretion of bile acids

2020 ◽  
Vol 2 (1) ◽  
Author(s):  
Priscila L. S. Alves ◽  
Jose De J Berrios ◽  
James Pan ◽  
Wallace H. Yokoyama
Keyword(s):  
Lithocholic Acid ◽  
Plasma Cholesterol ◽  
Liver Cholesterol ◽  
Control Group ◽  
Chow Diet ◽  
High Fat ◽  
Bean Flour ◽  
Freeze Dried ◽  
High Fat Diets ◽  
Fat Diets

Abstract Hypolipidemic and anti-obesity properties of extruded black, pinto and white beans were examined in male Syrian hamsters fed high fat diets for 3 weeks. The efficiency and effectiveness of extruder processing to eliminate heat-labile antinutrients were also determined. Hamsters fed a high fat diet containing 40% extruded black beans gained the same amount of weight as animals on a low-fat chow diet (based on AIN-93G). Total plasma cholesterol of the hamsters fed bean flour- based diets tended to be lower, ranging between 298 and 356 mg/dL, compared to 365 mg/dL for the control group and total and free liver cholesterol was about 50 and 33% lower, respectively, in the livers (freeze-dried) of hamsters fed the black turtle bean (BB) and pinto bean (PB) diets. The nitrogen content of the feces from hamsters fed the PB and white bean (WB) diets were 2.6x higher than the control and the excretion of deoxycholic acid (DCA) and lithocholic acid (LCA) was higher in all legume fed hamsters The results show that extrusion efficiently and economically reduces anti-nutritive factors that inhibit the digestion and absorption of proteins and carbohydrates. Graphical abstract

scholarly journals Copper bioavailability, mineral utilization, and lipid metabolism in broilers

2019 ◽  
Vol 64 (No. 12) ◽  
pp. 483-490
Author(s):  
Aiyou Wen ◽  
Sifa Dai ◽  
Xuezhuang Wu ◽  
Zhihua Cai
Keyword(s):  
Lipid Metabolism ◽  
Copper Sulphate ◽  
Plasma Lipids ◽  
Plasma Cholesterol ◽  
Average Daily Gain ◽  
Density Lipoprotein ◽  
Relative Bioavailability ◽  
Lipoprotein Cholesterol ◽  
Control Group ◽  
Mineral Utilization

The study was conducted to investigate the effects of copper (Cu) sources and levels on mineral utilization, tissue copper residues, and lipid metabolism in Arbor Acres broilers. A total of 640 male broilers were randomly divided into 5 groups with 8 replicates per group and 16 broilers per replicate. The experiment was used in a 2 × 2 + 1 factorial experiment design. Broilers in the control group were fed a basal diet, and animals in the other four groups were fed basal diets supplemented with Cu from copper sulphate and copper methionine. Copper concentrations of the experimental diets were 20 and 40 mg Cu/kg dry matter. A metabolism trial of 4 days was conducted during the last week of experimental feeding. Neither Cu source nor Cu level affected average daily gain, average daily feed intake or feed gain ratio (P &gt; 0.05). Broilers fed 40 mg Cu/kg diets had lower plasma cholesterol than those in the control group (P &lt; 0.05). Copper supplementation decreased (P &lt; 0.05) plasma low-density lipoprotein cholesterol but did not alter plasma high-density lipoprotein cholesterol concentrations or plasma triglyceride concentrations. Copper sulphate supplementation increased (P &lt; 0.05) liver Cu but did not alter pectorals Cu, heart Cu, tibia Cu and tibia P. Broilers fed 40 mg/kg Cu from copper sulphate had a lower (P &lt; 0.05) tibia Ca level. The concentration of liver Cu in the broilers fed copper methionine diets was higher (P &lt; 0.05) than that in those fed copper sulphate. Compared with copper sulphate (100%), the relative bioavailability value of copper methionine was 117%. In conclusion, the relative bioavailability of copper methionine obtained in this study was greater than that of copper sulphate. Copper plays an important role in plasma lipids and in the digestion of dietary Ca in broiler chickens.

Effect of Prunus Dulcis & Álpha-Tocopherol in Ethanol Induced Dyslipidemia In Wistar Rats

2020 ◽  
Vol 13 (4) ◽  
pp. 1619-1624
Author(s):  
Samuel Sundar Doss ◽  
J. Vijayakumar ◽  
E. Sukumar ◽  
K. Rekha
Keyword(s):  
Wistar Rats ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Prunus Dulcis ◽  
Alpha Tocopherol ◽  
Control Group ◽  
P Value ◽  
Low Density ◽  
Ethanol Group ◽  
Beneficial Effects

The study is aimed at assessing the effect of Prunus dulcis and alpha-tocopherol treatment against ethanol induced dyslipidemia in Wistar rats. 30 albino Wistar rats were selected based on the selection criteria and equally distributed into 5 groups – Control, ethanol, Prunus dulcis, alpha-tocopherol and combination of alpha-tocopherol + Prunus dulcis treated for 40 days. After the treatment for 40 days, all the animals were euthanized and a retro-orbital puncture was made to collect the blood samples for biochemical investigations. Obtained results were statistically analysed using ANOVA. Compared to ethanol group alpha tocopherol, Prunus dulcis and alpha tocopherol + Prunus dulcis treatment significantly decreased total cholesterol and triglycerides levels with p value <0.001. High density lipoprotein (66.31%) levels in the ethanol group were decreased compared to the control group and were significantly increased in other groups. Low density lipoprotein and Very low density lipoprotein levels were higher in the ethanol group compared with the control group and were significantly reduced in other groups with p value <0.001. Results suggest that ethanol has an ill effect on the lipid profile. Treatment with Prunus dulcius and alpha-tocopherol both solely or in combination has produced beneficial effects against dyslipidemia.

scholarly journals Dietary cholesterol reduces lipoprotein lipase activity in the atherosclerosis-susceptible Bio F1B hamster

2003 ◽  
Vol 89 (3) ◽  
pp. 341-350 ◽  
Author(s):  
Martina A. McAteer ◽  
David C. Grimsditch ◽  
Martin Vidgeon-Hart ◽  
G. Martin Benson ◽  
Andrew M. Salter
Keyword(s):  
Lipoprotein Lipase ◽  
Lipase Activity ◽  
Plasma Cholesterol ◽  
Dietary Cholesterol ◽  
Lipoprotein Metabolism ◽  
Coconut Oil ◽  
Hdl Cholesterol ◽  
High Cholesterol Diet ◽  
Lipoprotein Lipase Activity ◽  
Golden Syrian Hamster

We have compared lipoprotein metabolism in, and susceptibility to atherosclerosis of, two strains of male Golden Syrian hamster, the Bio F1B hybrid and the dominant spot normal inbred (DSNI) strain. When fed a normal low-fat diet containing approximately 40 g fat and 0·3 g cholestero/g, triacylglycerol-rich lipoprotein (chylomicron+VLDL) and HDL-cholesterol were significantly higher (P<0·001) in Bio F1B hamsters than DSNI hamsters. When this diet was supplemented with 150 g coconut oil and either 0·5 or 5·0 g cholestero/g, significant differences were seen in response. In particular, the high-cholesterol diet produced significantly greater increases in plasma cholesterol and triacylglycerol in the Bio F1B compared with the DSNI animals (P=0·002 and P<0·001 for cholesterol and triacylglycerol, respectively). This was particularly dramatic in non-fasting animals, suggesting an accumulation of chylomicrons. In a second experiment, animals were fed 150 g coconut oi/g and 5·0 g cholestero/g for 6 and 12 months. Again, the Bio F1B animals showed dramatic increases in plasma cholesterol and triacylglycerol, and this was confirmed as primarily due to a rise in chylomicron concentration. Post-heparin lipoprotein lipase activity was significantly reduced (P<0·001) in the Bio F1B compared with the DSNI animals at 6 months, and virtually absent at 12 months. Bio F1B animals were also shown to develop significantly more (P<0·001) atherosclerosis. These results indicate that, in the Bio F1B hybrid hamster, cholesterol feeding reduces lipoprotein lipase activity, thereby causing the accumulation of chylomicrons that may be associated with their increased susceptibility to atherosclerosis.

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