scholarly journals Genetics of plasma transferrins in the mouse

1960 ◽  
Vol 1 (3) ◽  
pp. 431-438 ◽  
Author(s):  
B. L. Cohen
Keyword(s):  
Gel Electrophoresis ◽  
Molecular Basis ◽  
Plasma Proteins ◽  
Inbred Strains ◽  
The Other ◽  
Starch Gel Electrophoresis ◽  
Widespread Distribution ◽  
Agouti Locus ◽  
Inbred Strains Of Mice ◽  
Starch Gel

1. The plasma proteins of six inbred strains of mice have been studied, using starch-gel electrophoresis.2. The existence of two alternative plasma transferrin (β-globulin) phenotypes has been demonstrated. Five of the strains have one of these and one strain has the other. Each of the two transferrin patterns comprises three (or possibly only two) electrophoretic bands. The two patterns differ in all of these bands.3. The two transferrin types recognized are determined by a pair of allelic, autosomal genes (designated TrfA and TrfB). The TrfA phenotype (CBA strain) is determined by the genotype TrfA/TrfA, and the TrfB phenotype (A, C57BL, JU, KL, RIII strains) by the genotype TrfB/TrfB. The phenotype TrfAB, of the heterozygote (genotype TrfA/TrfB), is distinguishable and shows four (or possibly only three) bands. In this way it closely resembles a mixture of equal parts of TrfA and TrfB plasma.4. No linkage was detected between the Trf locus and sex, the agouti locus or the haemoglobin locus.5. The possible molecular basis of the action of the transferrin alleles in the mouse, and the widespread distribution in mammals of polymorphism involving the transferrins, are discussed.

Study of Mouse Hemoglobin by Starch-Gel Electrophoresis

1964 ◽  
Vol 13 (2) ◽  
pp. 185-189 ◽  
Author(s):  
T. N. Mehrotra ◽  
Giuseppe Cardinali
Keyword(s):  
Gel Electrophoresis ◽  
Adult Animal ◽  
Electrophoretic Pattern ◽  
Inbred Strains ◽  
Single Band ◽  
Starch Gel Electrophoresis ◽  
Inbred Strains Of Mice ◽  
Starch Gel ◽  
Band Type

SummaryThe hemoglobin pattern of 18 inbred strains of mice was studied by starch-gel electrophoresis. In 7 strains (C57BL/10, C57BL/6, C57BR/cd, C57L, C58, SWR, and SEC/1Re) the electrophoretic pattern was found to be of single-band type: in the remaining 11 strains (AKR, DBA/1, DBA/2, CBA, C3H/He, C3HeB/Fe, Fl/1Re, RF, 129, A, and A/He), 6 components were constantly observed when the electrophoretic run was carried out for 20 hours. Hemoglobin from late C57BL/6 fetuses showed an electrophoretic pattern identical to that of the adult animal. Hemoglobin from AKR and DBA/2 late fetuses and newborns showed an electrophoretic pattern similar to that of the adult animal, but the slowest band was more intensely stained as compared with the corresponding band of the adult animal.

Identification of Australian barley cultivars by laboratory methods: gel electrophoresis and gel isoelectric focusing of the endosperm proteins

1977 ◽  
Vol 17 (89) ◽  
pp. 1020 ◽  
Author(s):  
J McCausland ◽  
CW Wrigley
Keyword(s):  
Isoelectric Focusing ◽  
Gel Electrophoresis ◽  
Water Soluble ◽  
The Other ◽  
Starch Gel Electrophoresis ◽  
Laboratory Methods ◽  
Barley Cultivars ◽  
Starch Gel ◽  
Electrophoretic Techniques ◽  
Gel Isoelectric Focusing

A range of laboratory methods was examined for their ability to distinguish between 19 barley cultivars currently grown in Australia. Aleurone colour, revealed after mechanical or chemical dehulling, differentiated Abyssinian, Atlas, Cape and Corvette from the other cultivars. Peroxidase and phenol testing were not useful. Seven different patterns were obtained for the hordeins of lowest mobility by starch gel electrophoresis. Further distinction was provided by flat gel isoelectric focusing of the water-soluble and hordein proteins for which 13 different pattern-groupings were obtained. The two electrophoretic techniques complemented one another, so that the use of both methods left only a few cultivars that could not be distinguished.

Inter-Species Relationships Within the Genus Oncorhynchus Based on Biochemical Systematics

1966 ◽  
Vol 23 (1) ◽  
pp. 101-107 ◽  
Author(s):  
H. Tsuyuki ◽  
E. Roberts
Keyword(s):  
Phylogenetic Relationships ◽  
Gel Electrophoresis ◽  
Plasma Proteins ◽  
Disc Electrophoresis ◽  
Starch Gel Electrophoresis ◽  
Species Relationships ◽  
Oncorhynchus Masou ◽  
Starch Gel ◽  
Specific Muscle ◽  
Species Specific

The species specific muscle myogens of Salmo gairdnerii, Oncorhynchus masou, O. masou ishikawae, O. kisutch, O. tshawytscha, O. keta, O. nerka, and O. gorbuscha are compared by starch gel electrophoresis. Plasma proteins of these same species are also examined by polyacrylamide disc electrophoresis. The range of usefulness of muscle myogens in species identification, and equally significantly, their value in establishing phylogenetic relationships of closely related groups, as the genus Oncorhynchus, are discussed. The myogen patterns of O. keta and O. gorbuscha from the Asiatic and North American coasts were found to be identical, further supporting the concept of absolute species specificity of these patterns.

STUDIES ON THE LIVETINS OF EGG YOLK: III. HETEROGENEITY AND IMMUNOELECTROPHORETIC BEHAVIOR OF BETA-LIVETIN

1966 ◽  
Vol 44 (10) ◽  
pp. 1357-1364 ◽  
Author(s):  
Shun-Fong Hui ◽  
R. H. Common
Keyword(s):  
Serum Albumin ◽  
Gel Electrophoresis ◽  
Egg Yolk ◽  
The Other ◽  
Positive Staining ◽  
Staining Reaction ◽  
Starch Gel Electrophoresis ◽  
Hen’S Egg ◽  
Starch Gel ◽  
The One

Starch-gel electrophoresis of the total livetins of hen's egg yolk resolved 16 zones: seven major zones, six minor zones, and three faint, diffuse zones. One zone was identified with the major component of paper electrophoretic alpha-livetin and hence with serum albumin. Four of the major zones were identified with the major components of paper electrophoretic beta-livetin on the one hand, and with an electrophoretically heterogeneous livetin antigen (livetin antigen 3) on the other hand, thus establishing the electrophoretic heterogeneity and relative immunological homogeneity of the paper electrophoretic beta-livetin fraction. The other two major starch-gel electrophoretic zones were identified as transferrins by their positive staining reaction for iron and comparison of their mobilities with two corresponding serum starch-gel fractions.

A Genetic Comparison of Sympatric Populations of Sand Lance (Genus Ammodytes) from the Region East of Cape Soya, Japan

1989 ◽  
Vol 46 (11) ◽  
pp. 1945-1951 ◽  
Author(s):  
Hiroaki Okamoto
Keyword(s):  
Genetic Structure ◽  
Gel Electrophoresis ◽  
Distinct Species ◽  
The Other ◽  
Starch Gel Electrophoresis ◽  
Sympatric Populations ◽  
Sand Lance ◽  
Protein Coding ◽  
Starch Gel ◽  
Genetic Comparison

Sand fance (Genus Ammodytes) collected from four stations off Japan and one station at Kodiak, Alaska were genetically characterized at 17 protein coding loci using starch-gel electrophoresis. Sand lance in Wakkanai (Cape Soya, Japan) consist of two genetically distinct groups. They are fixed for different alleles at four loci (Ldh-2, -3, G3pdh-2, and Mdhp-2). The genetic structure of one of the groups (Wakkanai-a group, W-a) is similar to that of A. personatus around Japan. The other group (Wakkanai-b group, W-b) has different genetic structure from either A. personatus or the Alaskan collection, which is presumed to belong to A. hexapterus. It is not presently possible to identify the affiliation of the W-b group; however, despite its sympatry with the W-a group, it is reproductively isolated and therefore is probably a distinct species occurring northeast of Hokkaido.

Patterns of evolution and migration in the arctic ground squirrel, Spermophilus parryii (Richardson)

1977 ◽  
Vol 55 (4) ◽  
pp. 748-758 ◽  
Author(s):  
Charles F. Nadler ◽  
Robert S. Hoffmann
Keyword(s):  
Body Size ◽  
Gel Electrophoresis ◽  
Ground Squirrel ◽  
The Other ◽  
The Arctic ◽  
Starch Gel Electrophoresis ◽  
Siberian Population ◽  
Spermophilus Parryii ◽  
Starch Gel ◽  
And Migration

Serum transferrins and 11 other genetically controlled proteins representing 17 loci were examined by starch-gel electrophoresis from Siberian, Alaskan, and Canadian populations of Spermophilus parryii. Six transferrin alleles were identified. Arctic populations (S. p. parryii, S. p. osgoodi) were characterized by Tf 6 occurring alone or together with Tf 7 whereas middle and subarctic populations exhibited Tf 7 occurring either alone (S. p. ablusus, S. p. lyratus, S. p. plesius) or together with Tf 5 (S. p. plesius). Tf 8, Tf 9, and Tf 19 constituted local variants. Tf 6 displayed a clinal distribution, increasing in frequency eastward and paralleling a clinal increase in body size. Three PGM2 alleles were observed, the frequencies of which tend to differentiate arctic S. p. parryii from subarctic S. p. ablusus. G6PD-b occurred uniformly in North America and in one Siberian population; a second population (two specimens) exhibited G6PD-a, thereby suggesting that G6PD polymorphism may be present in Siberian S. parryii. The other nine proteins were monomorphic in all Holarctic populations.

CONFIRMATION OF PLASMA PROTEIN CHANGES IN AVIAN ERYTHROBLASTOSIS

1964 ◽  
Vol 42 (2) ◽  
pp. 293-297 ◽  
Author(s):  
M. Merriman ◽  
C. le Q. Darcel
Keyword(s):  
Plasma Protein ◽  
Gel Electrophoresis ◽  
Plasma Proteins ◽  
Starch Gel Electrophoresis ◽  
Starch Gel

Alterations of the plasma proteins have been previously demonstrated in avian erythroblastosis by paper and now by starch gel electrophoresis. With the latter technique, eight protein zones are recognized in normal plasmas. Heparin contributes an additional non-staining zone. In leukemic plasmas two more zones occur while another zone shows significant retardation.Heparin is not responsible for these changes because they are also observed in oxalated plasmas, but there is evidence of increased binding of heparin in leukemic plasma.

A COMPARISON OF HISTONES FROM CHICKEN TISSUES BY ZONE ELECTROPHORESIS IN STARCH GEL

1961 ◽  
Vol 39 (3) ◽  
pp. 485-491 ◽  
Author(s):  
J. M. Neelin ◽  
G. C. Butler
Keyword(s):  
Cell Differentiation ◽  
Ionic Strength ◽  
Gel Electrophoresis ◽  
The Other ◽  
Starch Gel Electrophoresis ◽  
Electrophoretic Patterns ◽  
Zone Electrophoresis ◽  
Starch Gel ◽  
Chicken Erythrocytes ◽  
Characteristic Zone

Histories were extracted at pH 1.7 from washed nuclei of chicken erythrocytes, spleen, liver, and testis and compared by starch-gel electrophoresis at pH 5.0, ionic strength 0.020. Spleen and liver histories displayed the most complex electrophoretic patterns with 18 zones each and differed only in relative proportions of certain zones. Erythrocyte histone contained a characteristic zone while lacking a group present in spleen and liver histones. Testis histone with only seven zones differed markedly from the other three. These results were consistent with chromatograms of erythrocyte, spleen, and liver histones on sodium IRC-50. The suggested correlation of tissue-specific histones with cell differentiation is discussed.

Sign in / Sign up

Export Citation Format

Share Document