Assessment of milk quality based on bovine BAF60c gene mutation

2018 ◽  
Vol 156 (4) ◽  
pp. 570-574
Author(s):  
J. Liao ◽  
T. Ku ◽  
Y. F. Liu ◽  
J. Zhao
Keyword(s):  
Milk Fat ◽  
Fragment Length Polymorphism ◽  
Raw Milk ◽  
Milk Quality ◽  
Quality Traits ◽  
Fat Percentage ◽  
Milk Samples ◽  
Polymerase Chain ◽  
Solids Content

AbstractMonitoring milk quality traits and the classification of raw milk are important steps for generating high-quality dairy products. Given the important roles of the BRG1/BRM-associated factor 60c (BAF60c) gene in the regulation of physiological growth and production, the objective of the current study was to analyse the association between the BAF60c gene and milk quality and establish a gene-based method for pre-evaluating raw milk quality. For this purpose, DNA was isolated from 507 milk samples and genotyped using the polymerase chain reaction-restricted fragment length polymorphism method. Milk quality traits including milk protein percentage (MPP), milk fat percentage (MFP), lactose percentage (LP) and total solids content (TSC) were also evaluated from the same 507 milk samples. The newly found 6060 T > C mutation of the BAF60c gene was associated significantly with MPP and LP, but not with MFP and TSC. The results demonstrated that this mutation could be used for the pre-evaluation of MPP and LP; therefore, raw milk could be graded according to different genotypes.

Fatty acid profile of milk from Nordestina donkey breed raised on Caatinga pasture

2021 ◽  
pp. 1-5
Author(s):  
Tayanna Bernardo Oliveira Nunes Messias ◽  
Susana Paula Alves ◽  
Rui José Branquinho Bessa ◽  
Marta Suely Madruga ◽  
Maria Teresa Bertoldo Pacheco ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Gas Chromatography ◽  
Milk Fat ◽  
Fat Content ◽  
Donkey Milk ◽  
Fat Percentage ◽  
Mid Infrared ◽  
Milk Samples ◽  
The Third ◽  
Commercial Viability

Abstract In this research communication we describe the composition of fatty acids (FA) present in the milk of the Nordestina donkey breed, and how they differ during lactation. Milk samples were taken from 24 multiparous lactating Nordestina donkeys that grazed the Caatinga, comprising 5 animals at each of around 30, 60 and 90 d in milk (DIM) and a further 9 animals ranging from 120 to 180 DIM. The milk fat content was analysed by mid infrared spectroscopy and the FA profile by gas chromatography. The milk fat percentage ranged from 0.45 to 0.61%. The main FA found in milk were 16:0 and 18:1c9. These did not differ among DIM classes and comprised 23% and 25% of total FA. Notably, the α-Linolenic acid (18:3 n-3) was the third most abundant FA and differed (P < 0.05) with DIM, being lowest in the 30 and 60 DIM samples (around 10.7% of total FA) and highest in the 60 and 90 DIM classes (around 14.6% of total FA). The low-fat content and the FA profile of the donkey milk gives it potential as a functional ingredient, which could help to preserve the commercial viability of the Nordestina donkey breed.

scholarly journals Molecular Characterization and Classification of Phytoplasmas Associated with Canola Yellows and a New Phytoplasma Strain Associated with Dandelions

Plant Disease ◽  
2001 ◽  
Vol 85 (1) ◽  
pp. 76-79 ◽  
Author(s):  
Keri Wang ◽  
Chuji Hiruki
Keyword(s):  
Fragment Length Polymorphism ◽  
Rflp Analysis ◽  
Universal Primers ◽  
Dna Fragments ◽  
Chain Reaction ◽  
Aster Yellows ◽  
Distinct Subgroup ◽  
16S Ribosomal Dna ◽  
Polymerase Chain

DNA isolated from symptomatic canola (Brassica napus, Brassica rapa) and dandelion (Taraxacum officinale) was used to amplify 16S ribosomal DNA fragments by polymerase chain reaction using two pairs of universal primers P1/P6 and R16F2n/R2. Restriction fragment length polymorphism (RFLP) analysis of the amplified DNA fragments using endonucleases AluI, HhaI, HpaII, MseI, RsaI, and Sau 3AI revealed two distinct types of phytoplasmas in canola with similar symptoms. One had the same RFLP profiles as the phytoplasmas in subgroup 16SrI-A, whereas the other one had RFLP profiles similar to those of phytoplasmas in subgroup 16SrI-B. Phytoplasmas were detected in symptomatic dandelion plants that were collected from canola and alfalfa fields where severe alfalfa witches'-broom occurred. Comparative studies indicated that two different phytoplasmas were associated with the dandelion plants. One was identified as a member of subgroup 16SrI-A, whereas another one was classified as a member of a distinct subgroup in the aster yellows group on the basis of the unique RFLP patterns.

Evaluation of Raw Milk Quality Tests

1978 ◽  
Vol 41 (5) ◽  
pp. 356-360 ◽  
Author(s):  
C. H. WHITE ◽  
W. T. GILLIS ◽  
D. L. SIMMLER ◽  
M. K. GALAL ◽  
J. R. WALSH ◽  
...  
Keyword(s):  
Raw Milk ◽  
Milk Quality ◽  
Large Percentage ◽  
The Other ◽  
Milk Samples ◽  
Naturally Occurring ◽  
The Mean

A study was done to evaluate raw milk quality tests in an attempt to answer some questions regarding values or standards by which milk acceptance is determined. Initially, of 315 individual producer samples, 226 (72%) had proteolytic psychrotrophs. More than 90% of these psychrotrophs were of the genus Pseudomonas. All samples were obtained from four dairy companies processing milk in Georgia. One dairy had individual producers whose milk samples contained a large percentage of thermoduric psychrotrophs. “Bitter” flavors were more prevalent in samples collected from this dairy than in samples from the other three dairies. The degree of measurable proteolysis (Hull value) was directly correlated with the incidence of the naturally occurring “bitter” flavor. The mean Hull value for milk from this dairy (30.62 μg/ml) was 12–14 μg higher than the other three means. The mean Hull value for the “bitter” samples was 46 μg/ml.

CORRELATION STUDIES OF MILKFAT CONTENT AS MEASURED BY THE MILKO-TESTER, BABCOCK, AND MOJONNIER METHODS1,2

1973 ◽  
Vol 36 (2) ◽  
pp. 92-93 ◽  
Author(s):  
C. W. Shannon ◽  
J. T. Cardwell ◽  
L. C. True
Keyword(s):  
Statistical Analysis ◽  
Correlation Coefficient ◽  
Analysis Of Variance ◽  
Milk Fat ◽  
Raw Milk ◽  
Prediction Equation ◽  
Fat Content ◽  
Correlation Studies ◽  
Milk Samples ◽  
Average Variation

To evaluate some of the methods used to estimate milk-fat content in raw milk, producer's milk samples were tested by the Milko-Tester, Babcock, and Mojonnier methods. One hundred eighty-eight individual producer's raw milk samples were analyzed in duplicate the Mlilko-Tester and Babcock methods. In addition, 34 of these samples were randomly selected and analyzed in duplicate by the Mojonnier method. The correlation coefficient for the 188 samples tested by the Babcock and Milko-Tester methods was 0.95. A prediction equation for calculating Babcock results based on Milko-Tester readings was developed. Statistical analysis of the results of the three methods showed highly significant (P &lt; 01) correlation coefficient between each pair of the methods. An analysis of variance showed no significant differences (P &gt; .05) among the three methods. Average variation between duplicate samples showed results from the Milko-Tester to have the least variation, while those from the Babcock procedure varied the most between duplicate determinations.

Association of milk quality parameters with teat and udder traits in Tharparkar cows

2017 ◽  
Author(s):  
M. Aurif Wagay ◽  
A. K.S. Tomar ◽  
S. A. Lone ◽  
Anup K. Singh ◽  
Carolina P.
Keyword(s):  
Cell Count ◽  
Negative Correlation ◽  
Milk Sample ◽  
Present Experiment ◽  
Somatic Cell Count ◽  
Quality Parameters ◽  
Milk Quality ◽  
Phenotypic Correlation ◽  
Quality Traits ◽  
Milk Samples

The present experiment was carried out to study the association of milk quality traits (fat, protein, lactose, solid not fat (SNF), total solids (TS) and somatic cell count (SCC)) with teat traits ((teat length (TL), teat diameter (TD), distance between teats (DbT) and teat tip height from ground (THFG)) and udder traits ((udder circumference= UC, udder depth (UD), udder width (UW) and udder height from ground (UHFG)), in Tharparkar cows. The experiment was carried out on 32 tharparkar cows of different parities and lactation stages. Morning and evening measurement of teat and udder traits was carried out before milking and approximately 30 ml of representative milk samples were taken from each of the functional quarters and one composite milk sample of each experimental animal fortnightly from the date of calving of the animal in clean sampling bottles. Fat% and SNF% showed a negative, significant (P>0.05) to highly significant (P>0.01) correlation with TD, UL and FUD. Protein% expressed a positive, significant (P>0.05) to highly significant (P>0.01) correlation with UC, FUD and DbT. Further, Fat%, SNF% and TS% expressed a positive, significant (P>0.05) to highly significant (P>0.01) correlation with THFG. SCC (×105/ml) showed a positive, significant (P>0.05) to highly significant (P>0.01) correlation with TL, TD, DbT, UC and UL, whereas it had a negative correlation with THFG, rear-udder depth (RUD) and UW. It was further observed that Fat% expressed a positive highly significant (P>0.01) phenotypic correlation with SNF% and TS%. SNF%, Lactose%, Protein% and TS% expressed a negative phenotypic correlation with SCC.

scholarly journals Quality of raw milk collected from Mymensingh town in Bangladesh

2016 ◽  
Vol 45 (2) ◽  
pp. 73-78 ◽  
Author(s):  
MS Uddin ◽  
MR Habib ◽  
MA Islam ◽  
S Afrin ◽  
MH Rashid
Keyword(s):  
Specific Gravity ◽  
Milk Fat ◽  
Raw Milk ◽  
Fat Content ◽  
Chemical Parameters ◽  
Total Solids ◽  
Milk Samples ◽  
Negative Results ◽  
Different Sources

The present study was conducted to know the chemical qualities and adulteration of fresh raw milk collected from local markets (Bhangnamari bazaar, Sutiakhali bazaar, Vabokhali bazaar, Shombhuganj bazaar and Mymensingh sadar bazaar) of Mymensingh sadar, Bangladesh. Milk samples were analyzed for chemical parameters (contents of acidity, total solids, solids-not-fat, fat, protein and ash) and adulteration (presence of formalin, sugar or starch). Also, lactometer reading was recorded to monitor the specific gravity of the collected milk samples. Result showed that the specific gravity of milk from different sources differed non-significantly. The total solids, fat and protein contents of all the samples were in normal range except the milk fat content collected from Shombhuganj bazaar (32.66±4.04 g/Kg) and Mymensingh sadar bazaar (30.66±4.51 g/Kg) which had less (p<0.01) than the minimum legal standard of milk fat (35.00 g/Kg). The solids-not-fat content of the milk samples collected from Bhangnamari bazaar (72.93±8.78 g/Kg) and Vabokhali bazaar (79.33±6.81 g/Kg) were less than that from Mymensingh sadar bazaar (95.67±11.72 g/Kg). Developed acidity was detected in the samples from Bhangnamari bazaar (0.180±0.03), Sutiakhali bazaar (0.187±0.04%) and Mymensingh sadar bazaar (0.180±0.02%), while other samples were found to the fresh (0.150-0.157%). All of the adulteration tests showed negative results. The results suggested that though there were some fluctuations in quality among the bazaars regarding the standard of parameters, the raw milk samples were of acceptable quality.Bang. J. Anim. Sci. 2016. 45 (2): 73-78

scholarly journals Quality Analysis of Milk in Kathmandu Valley

2018 ◽  
Vol 5 ◽  
pp. 7-10
Author(s):  
Astha Parajuli ◽  
Prasiddhi Rimal ◽  
Rujisha Maharjan ◽  
Richa Chaudhary ◽  
Shashi Bhusan Chaturwedi
Keyword(s):  
Milk Sample ◽  
Milk Fat ◽  
Raw Milk ◽  
Fat Content ◽  
Quality Analysis ◽  
Kathmandu Valley ◽  
Microbiological Analysis ◽  
Milk Samples ◽  
Quality Of Milk

Objectives: This study was carried out to evaluate physiochemical, adulteration and microbial quality of milk sold in Kathmandu Valley. Methods: The study was carried out in Microbiology Laboratory of DAV College. The total of 20 milk samples randomly collected from different places of the valley including 10 pasteurized milk sample and 10 were raw milk sample, were processed for Physiochemical and Microbiological analysis. Result: The laboratory analysis revealed that the pasteurized samples has less mesophilic count as well as coliform count than raw milk samples. About 55% milk samples showed neutralizer test positive and 10% of milk samples were found to be positive for sugar test. However, none of the samples were found to contain starch as an adulterant. The average fat content of milk samples of Kathmandu Valley was 3%. Fat percent was significantly different among different sources of sampling points. The highest milk fat content value was recorded at Pulchowk (3.7%). The average SNF of Kathmandu Valley was 7% in which the pasteurized sample had the highest average SNF (7.3%) and the raw milk had lowest average SNF (6.8%). Conclusion: The significant variation in the physiochemical properties and microbial properties of the milk samples showed that people should be conscious about the consumption of market milk.  

scholarly journals Haptoglobin and serum amyloid A in bulk tank milk in relation to raw milk quality

2009 ◽  
Vol 76 (4) ◽  
pp. 483-489 ◽  
Author(s):  
Maria Åkerstedt ◽  
Karin Persson Waller ◽  
Åse Sternesjö
Keyword(s):  
Whey Protein ◽  
Acute Phase ◽  
Serum Amyloid A ◽  
Acute Phase Proteins ◽  
Raw Milk ◽  
Milk Quality ◽  
Bulk Tank Milk ◽  
Amyloid A ◽  
Milk Samples ◽  
Bulk Tank

The aim of the present study was to evaluate relationships between the presence of the two major bovine acute phase proteins haptoglobin (Hp) and serum amyloid A (SAA) and raw milk quality parameters in bulk tank milk samples. Hp and SAA have been suggested as specific markers of mastitis but recently also as markers for raw milk quality. Since mastitis has detrimental effects on milk quality, it is important to investigate whether the presence of Hp or SAA indicates such changes in the composition and properties of the milk. Bulk tank milk samples (n=91) were analysed for Hp, SAA, total protein, casein, whey protein, proteolysis, fat, lactose, somatic cell count and coagulating properties. Samples with detectable levels of Hp had lower casein content, casein number and lactose content, but higher proteolysis than samples without Hp. Samples with detectable levels of SAA had lower casein number and lactose content, but higher whey protein content than samples without SAA. The presence of acute phase proteins in bulk tank milk is suggested as an indicator for unfavourable changes in the milk composition, e.g. protein quality, due to udder health disturbances, with economical implications for the dairy industry.

465. Studies on variations in the solids-not-fat content of milk: I. Variations in lactation yield, milk-fat percentage and solids-not-fat percentage, that are due to the age of the cow

1952 ◽  
Vol 19 (1) ◽  
pp. 89-101 ◽  
Author(s):  
G. L. Bailey
Keyword(s):  
Milk Yield ◽  
Negative Correlation ◽  
Coefficient Of Variation ◽  
Milk Fat ◽  
Age Groups ◽  
Fat Content ◽  
Milk Quality ◽  
Age Group ◽  
Fat Percentage ◽  
Lactation Record

1. The coefficient of variation of lactation milk yield was approximately 25% for all age groups. That for lactation milk-fat percentage approximately 10% and that for lactation solids-not-fat percentage only 3%. The last two coefficients tended to increase with the age of the cow.2. Lactation milk yield was least with first calvers and rose to a maximum for sixth calvers. Milk quality was highest with first calvers.3. No tendency to vary with the season of calving could be found for any lactation record of any age group.4. During the first years of the herd's existence there was a small negative correlation between lactation milk yield and lactation milk-fat percentage. This has disappeared in more recent years.5. There was no correlation between lactation milk yield and lactation solids-not-fat percentage.6. There was a small (r = +0·40 approximately) positive correlation between lactation milk-fat percentage and lactation solids-not-fat percentage.

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