Molecular detection of natural infection ofLymnaea(Pseudosuccinea)columella(Gastropoda: Lymnaeidae) withFasciola gigantica(Digenea: Fasciolidae) from two provinces of South Africa

2019 ◽  
Vol 94 ◽  
Author(s):  
M.P. Malatji ◽  
S. Mukaratirwa
Keyword(s):  
South Africa ◽  
Intermediate Host ◽  
Molecular Detection ◽  
Natural Infection ◽  
Wide Distribution ◽  
Sub Saharan Africa ◽  
Snail Species ◽  
Eastern Cape ◽  
Kwazulu Natal ◽  
Sub Saharan

AbstractThe main intermediate host ofFasciola giganticain sub-Saharan Africa isLymnaea(Radix)natalensis.Lymnaea(Pseudosuccinea)columellais capable of transmitting bothF. giganticaandF. hepaticaand has been reported to be present in South Africa. To date, no natural infection withF. giganticahas been reported despite the wide distribution of the snail. The aim of this study was to confirm whetherL.(P.)columellawas transmittingF. giganticaand/orF. hepaticain selected locations of KwaZulu-Natal and Eastern Cape provinces of South Africa.Lymnaea(Pseudosuccinea)columellasnails were collected from two locations in two provinces of South Africa and screened for cercariae shedding. This was followed by humanely sacrificing the screened snails, and whole tissue of each individual snail was homogenized and amplified using primers designed to amplify the ITS-1 region ofFasciolaspp. No cercariae were shed from the screened snails and molecular analysis showed that snails from the two locations were infected withF. gigantica. This study confirms natural infection ofL.(P.)columellawithF. giganticain South Africa, whereF. giganticaandF. hepaticahave already been reported to coexist. AlthoughL.(P.)columellais able to transmit the two species, surprisingly no infection withF. hepaticawas detected from the screened snails. The natural intermediate host ofF. giganticain southern Africa, including South Africa, isLymnaea(Radix)natalensisand comparative studies are needed to determine the competence of the two snail species in the transmission ofF. gigantica.

scholarly journals Emergence of rabies in the Gauteng Province, South Africa: 2010–2011

2013 ◽  
Vol 84 (1) ◽  
Author(s):  
Claude T. Sabeta ◽  
Jacqueline Weyer ◽  
Peter Geertsma ◽  
Debra Mohale ◽  
Jacobeth Miyen ◽  
...  
Keyword(s):  
South Africa ◽  
Sub Saharan Africa ◽  
Domestic Dog ◽  
Eastern Cape ◽  
Gauteng Province ◽  
Canine Rabies ◽  
The Past ◽  
Outbreak Period ◽  
Kwazulu Natal ◽  
Sub Saharan

Canine rabies is enzootic throughout Sub-Saharan Africa, including the Republic of South Africa. Historically, in South Africa the coastal provinces of KwaZulu-Natal and Eastern Cape were most affected. Alarmingly, outbreaks of canine rabies have been increasingly reported in the past decade from sites where it has previously been under control. From January 2010 to December 2011, 53 animal rabies cases were confirmed; these were mostly in domestic dogs from southern Johannesburg, which was previously considered to be rabies free. In addition, one case was confirmed in a 26-month old girl who had been scratched by a pet puppy during this period. The introduction of rabies into Gauteng Province was investigated through genetic analysis of rabies positive samples confirmed during the outbreak period. In addition, the nucleotide sequences of incidental cases reported in the province for the past ten years were also included in the analysis. It was found that the recent canine rabies outbreak in the Gauteng Province came from the introduction of the rabies virus from KwaZulu-Natal, with subsequent local spread in the susceptible domestic dog population of southern Johannesburg. The vulnerability of the province was also highlighted through multiple, dead-end introductions in the past ten years. This is the first report of a rabies outbreak in the greater Johannesburg area with evidence of local transmission in the domestic dog population.

Paragonimiasis in KwaZulu-Natal province, South Africa

2012 ◽  
Vol 88 (1) ◽  
pp. 123-128 ◽  
Author(s):  
C.C. Appleton
Keyword(s):  
South Africa ◽  
Intermediate Host ◽  
Snail Species ◽  
Sufficient Information ◽  
Suspected Case ◽  
Melanoides Tuberculata ◽  
Kwazulu Natal ◽  
Second Intermediate Host ◽  
Adult Female Patient ◽  
Prosobranch Snail

AbstractNine cases of paragonimiasis have been reported from cats (4), dogs (2) and children (3) in South Africa, with an additional suspected case in an adult female patient. Details of these cases are reviewed. All nine cases, and perhaps the adult case as well, were from the province of KwaZulu-Natal but locality data are only available for six of them. These six cases represent four localities which all lie below 100 m above sea level in the province's lowlands, suggesting that there may be a focus of transmission here. The molluscan first intermediate host must be one of the two prosobranch snail species present in the area,Melanoides tuberculataorTomichia natalensis, and the decapod second intermediate host the common river crabPotamonautes sidneyi. All infected cats and dogs had pulmonary infections, while two human cases for which there is sufficient information had extrapulmonary infections. Transmission appears to be ongoing but the invasive snailTarebia graniferamay be competing with bothM. tuberculataandT. natalensis.If so, this may bring transmission to an end.

scholarly journals Factors influencing uptake of schistosomiasis research findings in ingwavuma area, uMkhanyakude District, Kwazulu-Natal, South Africa

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Tafadzwa Mindu ◽  
Moses J. Chimbari
Keyword(s):  
South Africa ◽  
Rural Areas ◽  
Health Workers ◽  
Sub Saharan Africa ◽  
Primary School Children ◽  
Factors Influencing ◽  
Kwazulu Natal ◽  
Research Uptake ◽  
Research Findings ◽  
Sub Saharan

Background: Research uptake is concerned with spreading ideas across multiple levels of the community. Barriers such as poverty, lack of infrastructure, illiteracy and culture prevent information sharing in arid rural areas of sub-Saharan Africa. Objective: This study explores the factors influencing schistosomiasis research uptake and the available channels for the uptake of research findings from a transdisciplinary and eco-health research project on schistosomiasis in Ingwavuma area, uMkhanyakude district, KwaZulu-Natal province in South Africa. Methods: This case study conducted in 2017 involved 78 primary school children and 73 heads of household recruited through convenience and purposive sampling. Data were collected through focus group discussions, then transcribed and analysed by the researcher using thematic analysis. Results: Factors such as poor knowledge, water and sanitation problems, and lack of sufficient health workers hindered the uptake of schistosomiasis research findings. Participants recommended several platforms to share schistosomiasis research findings with the community, including: door to door visits; social gatherings such as sports events, talent shows, and religious gatherings; mass media platforms such as radio and television; social media platforms such as WhatsApp, Facebook and Twitter; and printed media such as posters, booklets and pamphlets. Conclusions: There is a need to train health workers and peer educators in this area of South Africa to educate people about schistosomiasis infection, screening and treatment through home visits or social events. Schistosomiasis research findings must be synthesised and packaged in different forms for dissemination via multimedia media-based communication channels.

scholarly journals Distribution and habitats of Bulinus tropicus, snail intermediate host of the conical fluke Calicophoron microbothrium, in South Africa

2002 ◽  
Vol 21 (4) ◽  
pp. 114-120
Author(s):  
K. N. De Kock ◽  
C. T. Wolmarans ◽  
M. Bornman ◽  
D. C. Maree
Keyword(s):  
South Africa ◽  
Intermediate Host ◽  
Geographical Distribution ◽  
Freshwater Snail ◽  
Wide Distribution ◽  
Limiting Factor ◽  
Snail Species ◽  
Rural Settlements ◽  
Economic Implications ◽  
Snail Intermediate Host

This paper focuses on the geographical distribution and the habitats of Bulinus tropicus, the snail intermediate host of the conical fluke, Calicophoron microbothrium. Bulinus tropicus is the freshwater snail species with the most extensive geographical distribution, as reflected by the collection sites of the 7 992 samples currently on record in the database of the National Freshwater Snail Collection (NFSC) of South Africa. The presence of this species was noted by collectors in a total of 14 different types of waterbodies, however, the largest number of samples was collected in dams and brooks and in habitats of which the water was described as standing, clear and fresh. The effect size was calculated for each variable separately to determine its importance in governing the distribution of this species. The data in the database were further processed and adapted to construct an integrated decision tree. This is a statistical model allowing the selection of those variables which can maximally discriminate between the frequency of occurrence of a given species and all other species in the database under specific conditions. The success of B. tropicus, as reflected by its extensive geographical distribution, can most probably be ascribed to the circumstance that population dynamic studies by various authors have shown, without any doubt, that it is highly r-selected and that it additionally has the ability to survive conditions of drought for elatively long periods. It does however, appear as if its limited tolerance to water with a relatively low conductivity could possibly be a limiting factor for its colonisation of specific areas in South Africa. As intermediate host of C. microbothrium which causes paramphistomosis in domestic stock and sometimes can lead to great losses, the wide distribution of B. tropicus has economic implications, especially for residents of informal, rural settlements whose livestock traditionally forms an integral part of their daily existence.

scholarly journals Hesperoides, a new “hairy” flea beetle genus from southern Africa (Coleoptera: Chrysomelidae, Galerucinae, Alticini)

2017 ◽  
pp. 151-158 ◽  
Author(s):  
Maurizio Biondi
Keyword(s):  
South Africa ◽  
Southern Africa ◽  
Flea Beetle ◽  
Extensor Tendon ◽  
Morphological Characters ◽  
Eastern Cape ◽  
Male And Female ◽  
Kwazulu Natal ◽  
Sub Saharan ◽  
The Republic

Hesperoides afromeridionalis gen. nov. and sp. nov. from the Republic of South Africa (Western and Eastern Cape Provinces and KwaZulu-Natal) is described. Despite some similarities with Hespera Weise, it exhibits major affinities with the genera attributed to the subtribe Aphthonini, especially with Aphthona Chevrolat and Montiaphthona Scherer. Data on distribution are supplied, along with preliminary ecological notes. Photomicrographs of main morphological characters, including male and female genitalia, and metafemoral extensor tendon are provided. Key to the six “hairy” flea beetle genera occurring in sub-Saharan African and their habitus photos are also given.

Classical and Molecular Cytogenetics of the Panther Genet Genetta maculata (Mammalia, Carnivora, Viverridae)

2016 ◽  
Vol 149 (4) ◽  
pp. 274-281 ◽  
Author(s):  
Raquel Matoso Silva ◽  
Filomena Adega ◽  
Helena J. Kjöllerström ◽  
Kim Labuschagne ◽  
Antoinette Kotze ◽  
...  
Keyword(s):  
South Africa ◽  
Molecular Cytogenetics ◽  
Wide Distribution ◽  
Sub Saharan Africa ◽  
Genomic Rearrangements ◽  
Gauteng Province ◽  
Telomere Repeat ◽  
Telomeric Sequences ◽  
Sub Saharan ◽  
Karyotypic Variation

Genets (Genetta) are a genus of African mammalian carnivorans with 14 currently recognized species, although taxonomic uncertainties remain, particularly regarding the number of species within the large-spotted genet complex. This study presents the first banded karyotype and molecular cytogenetic analysis of a genetically identified panther genet, Genetta maculata, the most common and widespread taxon of the large-spotted genet complex, with a wide distribution in sub-Saharan Africa. Sampled in Gauteng Province, South Africa, it could be assigned to the subspecies G. m. letabae on geographic grounds and had a similar karyotype (2n = 52, FNa = 96) to those published for a panther genet from Ethiopia and for the West African large-spotted genet G. pardina. Notably, the specimen had a different autosomal morphology (2 acrocentric chromosomes) from that previously attributed to letabae (a single acrocentric chromosome), but the latter assignment was uncertain because the studied individuals were captive born and assigned based solely on a presumed origin in the former Transvaal Province of South Africa. Fluorescence in situ hybridization with a telomere repeat probe revealed the presence of telomeric sequences in the centromeres of most chromosomes, the so-called interstitial telomeric sites (ITSs). Since genets seem to have a unique, highly rearranged karyotype among feliforms and relatively low interspecific karyotypic variation, and considering the known instability of ITSs, we suggest that the large amount of ITSs found here might be due to evolutionarily recent extensive genomic rearrangements. This study provides cytogenetic information that contributes to our understanding of chromosomal variation and genomic rearrangements in genets, and valuable baseline data for future studies of karyotype evolution in carnivores in general and viverrids in particular.

scholarly journals Occurrence of Theileria parva and other haemoprotozoa in cattle at the edge of Hluhluwe-iMfolozi Park, KwaZulu-Natal, South Africa

2010 ◽  
Vol 81 (1) ◽  
Author(s):  
S. B.A.S. Yusufmia ◽  
N. E. Collins ◽  
R. Nkuna ◽  
M. Troskie ◽  
P. Van Den Bossche ◽  
...  
Keyword(s):  
South Africa ◽  
Buffy Coat ◽  
Babesia Bovis ◽  
Sub Saharan Africa ◽  
Significant Finding ◽  
Theileria Parva ◽  
Mortality And Morbidity ◽  
Kwazulu Natal ◽  
Pcr Rflp ◽  
Sub Saharan

Theileria parva, the most important bovine theilerial species in sub-Saharan Africa, causes widespread mortality and morbidity in endemic areas. A survey was conducted using buffy-coat specimens from 60 apparently healthy adult communally herded Nguni-type cattle at the northeastern edge of the Hluhluwe-iMfolozi Park to determine, by means of PCR and Reverse Line Blot (RLB) hybridisation, the occurrence of Theileria and Babesia species. The presence of Trypanosoma species was determined using PCR-RFLP. Results showed that 6.7 % of the specimens were positive for Theileria parva. This significant finding suggests that cattle in South Africa, and not only African buffaloes (Syncerus caffer), may be subclinical carriers of T. parva. Other species identified were T. mutans (83.3 %), T. velifera (70.0 %), Theileria sp. (sable) (46.8 %) and T. taurotragi (1.7 %). Two specimens (3.3 %) were positive for Babesia bovis and single specimens (1.7 %) positive for B. bigemina and B. rossi, respectively. Mixed infections, of up to 4 species, were common (65.0 %). Only 1 specimen was found to be positive for Trypanosoma vivax, and 2 for T. theileri, of which only the first species is pathogenic.

scholarly journals Complications of traditional circumcision amongst young Xhosa males seen at St Lucy’s Hospital, Tsolo, Eastern Cape, South Africa

2013 ◽  
Vol 5 (1) ◽  
Author(s):  
Ugochukwu Anike ◽  
Indiran Govender ◽  
John V. Ndimade ◽  
John Tumbo
Keyword(s):  
South Africa ◽  
Peer Pressure ◽  
Sub Saharan Africa ◽  
Eastern Cape ◽  
Excessive Bleeding ◽  
Cross Sectional ◽  
Genital Mutilation ◽  
Increased Risk ◽  
Hospital Stays ◽  
Sub Saharan

Background: Traditional circumcision of males is common amongst many societies in sub-Saharan Africa. Circumcision amongst the Xhosa people of South Africa represents a rite of passage to manhood. Traditional male circumcision has an increased risk for complications that include sepsis, genitalmutilation, gangrenous penis, excessive bleeding, dehydration, renal failure and death. The aim of this study was to describe the complications of traditional circumcisions amongst Xhosa men as seen at St. Lucy’s Hospital in the Eastern Cape Province.Method: A cross-sectional descriptive quantitative study was conducted in 2008. Records of 105 malesadmitted to St. Lucy’s Hospital with complications following traditional circumcision were reviewed. Data collected included age, education level, race, reasons for circumcision, complications, the period of circumcision, duration of hospital stay and the outcomes. Descriptive data analysis was performed using statistical software SPSS 17.0.Results: The ages ranged from 15–35 years with 68 (64.8%) between 15–19 years. 83 (79%) had a secondarylevel of education, 16 (15.2%) primary, 5 (4.8%) tertiary and 1% had no education. 60 (57%) werecircumcised as initiation to manhood, 21 (20.0%) due to peer pressure, 20 (19.0%) for cultural reasons, and 1(1.0%) was forced. The complications were sepsis (59 [56.2%]), genital mutilation (28 [26.7%]), dehydration(12 [11.4%]) and amputation of genitalia (6 [5.7%]).Fifty-nine (56.2%) patients were circumcised in winter.79 (75.2%) were circumcised in the forest, and 25 (23.8%) in initiation centres. Fifty-eight (55.2%) werecircumcised by traditionalists, and 47 (44.8%) by tribal elders (initiators). Hospital stays ranged from 8 to28 days. 66% were healed and discharged, and 29 (27.6%) were referred to higher centres of care.Conclusion: Genital sepsis was the most common complication of traditional male circumcision.Complications were related to the circumciser, advanced age of the patient and place of circumcision. Thereis need for training of the traditional circumcisers on safe techniques and use of hygienic practices in orderto reduce the complications identified in this study.

scholarly journals Temperature at conception and pregnancy loss in rural KwaZulu-Natal Province, South Africa: Implications for climate change policy in sub-Saharan African settings

2021 ◽  
Author(s):  
Yoshan Moodley ◽  
Frank Tanser ◽  
Andrew Tomita
Keyword(s):  
South Africa ◽  
Global Warming ◽  
Reproductive Health ◽  
South African ◽  
Pregnancy Loss ◽  
Sub Saharan Africa ◽  
Kwazulu Natal ◽  
Sub Saharan ◽  
Women’S Reproductive Health ◽  
Women's Reproductive Health

AbstractBackgroundGlobal warming is projected to cause a substantial rise in temperatures with serious health implications across sub-Saharan Africa. Although South African policy makers have drafted a climate change adaptation plan, potential health threats posed by increasing temperatures on women’s reproductive health are overlooked due to the lack of local population-based evidence. We sought to address the gap in the evidence around global warming and women’s reproductive health in sub-Saharan Africa by using one of the continent’s largest prospective cohorts from rural KwaZulu-Natal Province, South Africa to investigate the relationship between temperature at conception and pregnancy loss.MethodsOur study sample consisted of 36341 pregnancies from 16765 women from the uMkhanyakude District of KwaZulu-Natal, South Africa between 2000-2017. Average monthly temperatures for the study locale during the study period were obtained from the South African Weather Services. An adjusted logistic regression model was used to investigate the relationship between temperature at conception and pregnancy loss (miscarriage or stillbirth).ResultsThe rate of pregnancy loss in the study sample was 1.9 (95% Confidence interval [CI]: 1.7-2.0) per 100 pregnancies. We observed a 4% higher odds of pregnancy loss for each 1°C increase in temperature (Adjusted Odds Ratio: 1.04, 95% CI: 1.01-1.08).ConclusionThere is a clear relationship between temperature and pregnancy loss in our sub-Saharan African setting. The effects of global warming will likely exacerbate the existing challenges for women’s reproductive health in this region. Pregnancy outcomes should be given adequate attention when sub-Saharan African governments draft policies in response to global warming.

Sign in / Sign up

Export Citation Format

Share Document