scholarly journals A survey of measles antibodies in different populations

1964 ◽  
Vol 62 (1) ◽  
pp. 45-51 ◽  
Author(s):  
Gillian Tilden Smith
Keyword(s):  
General Practitioners ◽  
Technical Assistance ◽  
Age Groups ◽  
Serum Samples ◽  
Past Infection ◽  
Rapid Acquisition ◽  
Young Infants ◽  
Measles Antibodies ◽  
Antibody Titres ◽  
Different Populations

A survey of serum samples from all age groups in the population has shown a rapid acquisition of antibody between the ages of 1 and 6 years which reaches a plateau in concentration and does not disappear later.The concentration of antibody in cord serum may be four or more times greater than that in the mother's serum.Presence or absence of a record of past infection by measles is not necessarily reflected by the titre of antibody in the serum.Low antibody titres in very young infants may be due to an imperfectly developed antibody producing mechanism.The author gratefully acknowledges the assistance of all those who made this survey possible. In particular, she is indebted to Dr G. I. Watson of the College of General Practitioners and his colleagues (see list) for their help in the collection of so many of the serum samples, and to Mr D. A. Bamgboye for his excellent technical assistance.

scholarly journals Determination of serum antibody titres and immune status of layer flocks against Newcastle Disease virus at Chittagong district of Bangladesh

1970 ◽  
Vol 1 (2) ◽  
pp. 35-38 ◽  
Author(s):  
MA Kashem ◽  
M Parvej ◽  
MA Hashem ◽  
MM Moula ◽  
ASMG Kibria
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Immune Status ◽  
Haemagglutination Inhibition ◽  
Serum Antibody ◽  
Age Groups ◽  
Serum Samples ◽  
Specific Immunity ◽  
Antibody Titres

A study was conducted to assess the level of serum antibody titres and immune status of layer birds against Newcastle Disease virus by Haemagglutination Inhibition (HI) test in different areas of Chittagong district during November to December, 2010. Sixteen layer flocks were selected based on different ages of birds. A total of 235 serum samples were collected and tested at Microbiology laboratory of CVASU. HI test was performed using commercial Newcastle Disease vaccine (Avinew®) as a source of 4HAU virus antigen. The antibody titre (GMT) levels in 18-26 weeks age group were found to be 70.198, followed by 47.551, 34.776, 17.281 and 18.855 in 27-40, 41-57, 58-73 and >73 weeks age groups, respectively. Moreover, 100% specific immunity against ND was found in 18-26, 27-40 and 41-57 weeks age groups of birds, whereas 93.33 and 94.73% specific immunity was found in 58-73 and >73 weeks age groups, respectively. On an average, 97.87% layer birds showed specific immunity and 2.13% showed nonspecific immunity against NDV. We considered HI titre of 1:8 or above as specific immunity and less than 1:8 as non specific immunity. Highest HI titre was found at the age of 18-26 weeks and lowest titre was at 58-73 weeks of age. The lower level of HI titre seemed to be directly related to some important factors relating to vaccination which have been highlighted in this paper. Key words: Antibody titers; Immune status; HI test; Newcastle disease virus; Layer birds. DOI: http://dx.doi.org/10.3329/ijns.v1i2.8818 International Journal of Natural Sciences (2011), 1(2):35-38

scholarly journals Human antibody response to fragments A and B of diphtheria toxin and a synthetic peptide of amino acid residues 141–157 of fragment A

1990 ◽  
Vol 105 (3) ◽  
pp. 457-468 ◽  
Author(s):  
V. Y. Perera ◽  
M. J. Corbel
Keyword(s):  
Amino Acids ◽  
Synthetic Peptide ◽  
Diphtheria Toxin ◽  
Age Groups ◽  
Human Antibody ◽  
Amino Acid Residues ◽  
High Antibody ◽  
Serum Samples ◽  
Antibody Titres ◽  
Selection Of

SUMMARYExamination of a selection of serum samples from adults from two regions of England showed that 50 % of men in the 16–24 years and over 55 years age groups had high titres of antibody to diphtheria toxin (DT). In contrast, only 11% of women aged 16 to over 55 years had high titres of antibody to DT. All human antisera with high anti-DT titres reacted with a synthetic peptide (SP) corresponding to the amino acids 141–157 of DT fragment A, with sera from men aged 35 to over 55 years showing the highest titres. High antibody titres to fragment A paralleled those to SP in both sexes. Titres of antibody to DT fragment B were highest in individuals with high titres to DT. In sera from both sexes immunoglobulin G1 was the predominant subclass reactive with all three antigens. However, both IgG1 and IgG4 and to a lesser extent IgG2 and IgG3 were present in immunoglobulin concentrates.

Seropositivity and epidemiology of human parechovirus types 1, 3, and 6 in Japan

2016 ◽  
Vol 144 (16) ◽  
pp. 3451-3460 ◽  
Author(s):  
K. WATANABE ◽  
C. HIROKAWA ◽  
T. TAZAWA
Keyword(s):  
Neutralizing Antibodies ◽  
Respiratory Diseases ◽  
Clinical Symptoms ◽  
Age Groups ◽  
Gastrointestinal Symptoms ◽  
Neutralizing Antibody ◽  
Young Infants ◽  
Antibody Titres ◽  
Type 3 ◽  
Increased Susceptibility

SUMMARYHuman parechoviruses (HPeVs) mainly infect young children, causing mild gastrointestinal and respiratory diseases; however, HPeV type 3 (HPeV3) causes severe systemic diseases in young infants. To clarify the characteristics of HPeV infections from the aspects of seropositivity and epidemiology, we measured neutralizing antibody titres against HPeVs in individuals of in different age groups and isolated HPeVs from various clinical specimens in Niigata, Japan. The seropositivity to HPeV1, 3, and 6 was higher in older age group. HPeV1 and HPeV6 seropositivities were maintained in adults, whereas HPeV3 seropositivity was significantly lower in subjects aged >40 years (P < 0·001, P = 0·003). This result suggests that adults have increased susceptibility to HPeV3 as they lack neutralizing antibodies against HPeV3. Of the HPeV isolates, HPeV1 and HPeV6 frequently caused gastrointestinal symptoms. Moreover, gastroenteritis patients with HPeV1 and HPeV6 were mainly aged 6 months–1 year and ⩾2 years, respectively. In contrast, only HPeV3 was isolated from neonates and young infants with sepsis or sepsis-like syndrome, often with respiratory symptoms. These results suggest that clinical symptoms are clinically related to HPeV genotype and patients’ age.

A new medical Internet system in healthcare: innovation of Telegeriatrics and healthcare demands of older patients of different age groups (Preprint)

2020 ◽  
Author(s):  
Yu Gong ◽  
Jianyuan Zhou
Keyword(s):  
Cardiovascular Diseases ◽  
General Practitioners ◽  
Older Patients ◽  
Metabolic Diseases ◽  
Treatment Plan ◽  
Age Groups ◽  
The Elderly ◽  
Diagnosis And Treatment ◽  
World Health ◽  
New Model

BACKGROUND Healthcare for older patients is a worldwide challenge for public health system. A new medical Internet system in healthcare which is a new model of telegeriatrics system has been established. The key innovation is the new telegeriatrics system was conducted jointly by general practitioners in the Community Health Service Center and specialists in university teaching hospital. Unlike the typical telemedicine that has been practiced in other countries, the new model provides a solution for the key issues in telemedicine where a doctor is unable to conduct a direct physical examination and the associated potential diagnostic error. OBJECTIVE This study is to introduce the operation mechanism of the new Telegeriatrics system and analyze healthcare demands of older patients in different age groups applying the new Telegeriatrics system. METHODS 472 older patients (aged≥60) were enrolled and divided into the young older group (aged 60 to 74), the old older group (aged 75 to 89) and the very old group (aged≥90) according to the age stratification of World Health Organization. Proportion of the top 10 diseases of older patients of different age groups was analyzed. RESULTS The process of older patients’ diagnosis and treatment made by specialist and general practitioners formed a closed loop. It ensures the timeliness and effectiveness of diagnosis and treatment of older patients. The treatment effect can be observed by general practitioners and specialist can adjust the treatment plan in time. In this study, it was found that older patients in different age groups have different healthcare demands. Coronary heart disease and type 2 diabetes mellitus were found to be the main diseases of the older patients and the young older patients as well as the old older patients applying Telegeriatrics. CONCLUSIONS The new telegeriatrics system can provide convenient and efficient healthcare services for older patients and overcome the disadvantage of currently used models of telegeriatrics. Older patients in different age groups have different medical care demands. Cardiovascular diseases and metabolic diseases have become the main diseases of the elderly applying the new Telegeriatrics system. Healthcare policy makers should invest more medical resources to the prevention of cardiovascular diseases and metabolic diseases in the elderly.

scholarly journals Reference values of plasma homocysteine in Cuban children and adults

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Alina Concepción Alvarez ◽  
Ivette Camayd Viera ◽  
Lisy Vento Buigues ◽  
Yanet Fernández Martínez ◽  
Eraida Hernández ◽  
...  
Keyword(s):  
Reference Values ◽  
High Performance ◽  
Age Groups ◽  
Coffee Consumption ◽  
Reference Interval ◽  
Reference Intervals ◽  
Multifactorial Diseases ◽  
Weak Association ◽  
Different Populations ◽  
Cuban Population

AbstractObjectivesHomocysteine (Hcy) is a nonessential amino acid, produced by the demethylation of methionine. High Hcy levels, or hyperhomocysteinemia, have been associated with genetic and multifactorial diseases. Hcy reference values may vary between different populations, as Hcy levels are affected by factors such as sex, age, diet, smoking, and coffee consumption. The estimation reference interval (RI) allows to establish the normal values of this marker in population. At present, these levels are unknown in Cuba. The aim of this work is to estimate the Hcy reference intervals in Cuban children and adults.MethodsTotal Hcy concentration was quantified by high performance liquid chromatography (HPLC) in plasma. Hcy levels were evaluated in samples from 507 healthy individuals (260 children, 247 adults).ResultsRIs were estimated by nonparametric methods. We found significant differences between both age groups, but we did not find significant differences between sexes, within these groups. The established ranges were 2.56–14.55 µM and 3.63–17.19 µM for children and adults, respectively. Also, we observed a weak association between Hcy levels and age in both sex groups.ConclusionsThis is the first study that assesses Hcy reference values in Cuban population. Our results will allow the introduction of Hcy as a biochemical marker in laboratory testing.

scholarly journals Antibodies anti-trypanosomatides in domestic cats in Paraná: who is at highest risk of infection?

2018 ◽  
Vol 27 (2) ◽  
pp. 232-236 ◽  
Author(s):  
Andressa Maria Rorato Nascimento de Matos ◽  
Eloiza Teles Caldart ◽  
Fernanda Pinto Ferreira ◽  
Keila Clarine Monteiro ◽  
Marielen de Souza ◽  
...  
Keyword(s):  
Antibody Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Domestic Cats ◽  
Serum Samples ◽  
Animal Group ◽  
Animal Protection ◽  
Leishmania Spp ◽  
In Series ◽  
Different Populations ◽  
Simple Logistic

Abstract The aim of this study were to detect antibodies anti-Leishmania spp. and anti-Trypanosoma cruzi in two different populations of domestic cats (Felis catus domesticus) from North Paraná referred for surgical castration and to determine which characteristics of the animals studied may be associated with seropositivity. Serum samples from 679 cats were analyzed using enzyme-linked immunosorbent assay (ELISA) and indirect immunofluorescence antibody test (IFAT) in series. Associations between age, sex, race, year of care and animal group were verified using the simple logistic regression. Percentage of 8.5% (58/679) of cats were positive for Leishmania spp. and 7.6% (51/673) for T. cruzi by the tests ELISA and IFAT. Animals collected by non-governmental animal protection organizations presented more seropositivity for Leishmania spp. (p<0.0001). Results shown that Leishmania spp. and T. cruzi are present in domestic cats in the northern part of the state of Paraná, as well as, owners of non-governmental animal protection organizations may be more exposed to leishmaniasis when compared to other animal owners evaluated in the present study.

Distribution of Human Cytomegalovirus Glycoprotein H Genotypes in Different Samples from Chinese Children

2021 ◽  
Author(s):  
Wei Li ◽  
Lu-Yan Chen ◽  
Ran Tao ◽  
Shi-Qiang Shang
Keyword(s):  
Breast Milk ◽  
Human Cytomegalovirus ◽  
Throat Swab ◽  
Quantitative Polymerase Chain Reaction ◽  
Age Groups ◽  
Urine Samples ◽  
Serum Samples ◽  
Predominant Genotype ◽  
Milk Samples ◽  
Glycoprotein H

Abstract Objective This study aimed to investigate characteristics of human cytomegalovirus (HCMV) glycoprotein H (gH) genotypes in urine, throat swab, and serum from children and breast milk from children's mothers. Methods Fresh urine samples, throat swabs, or serum samples from children and breast milk samples from children's mothers were collected for HCMV DNA detection. The positive samples of HCMV DNA were further detected by fluorescent quantitative polymerase chain reaction (PCR) with gH typing. Results Of 1,703 HCMV DNA-positive samples, the highest proportion (83.3%, 85/102) of children aged between 21 days and 3 months was detected positive in breast milk samples (p = 0.002), and the highest proportion (70.5%, 110/156) of children aged above 3 months was detected positive in throat swab samples (p = 0.002). HCMV in throat swab specimens is mainly high copy (p < 0.0001), and low-copy HCMV is prevalent in breast specimens (p < 0.0001). Among them, 1,059 samples were identified as gH1 genotype, 530 samples were gH2, and 114 samples were coinfection (gH1/2). There had the highest gH2 rates (32.3%) and lowest gH1 (61.0%) rates in urine samples (p = 0.041), whereas the highest gH1 rates (71.6%) and lowest gH2 rates (19.6%) were found in breast milk samples (p = 0.032). Concerning age groups, patients aged between 21 days and 3 months had the highest gH1 proportion (p = 0.017), while patients aged above 3 months had the highest gH1 and gH2 HCMV coinfection proportion (p = 0.002). Among 43 pairs of maternal and child samples corresponding to positive samples, gH genotype of 35 pairs of samples was consistent with a rate of 81.4%. Conclusion gH1 is the predominant genotype of HCMV in each kind of sample in China. However, the distribution of the HCMV gH genotype is different among different samples.

scholarly journals Prevalence of Brucellosis in Dairy Cattle in Organized and Smallholder Farms in Some Selected Areas of Bangladesh

2014 ◽  
Vol 12 (2) ◽  
pp. 167-171
Author(s):  
MAS Sarker ◽  
MS Rahman ◽  
MT Islam ◽  
AKMA Rahman ◽  
MB Rahman ◽  
...  
Keyword(s):  
Age Groups ◽  
Dairy Farm ◽  
Economic Loss ◽  
Screening Tests ◽  
Ring Test ◽  
Serum Samples ◽  
Smallholder Farms ◽  
Three Samples ◽  
Holstein Friesian ◽  
Confirmatory Tests

Brucellosis causes a great economic loss to the livestock industries through abortion, infertility,birth of weak and dead offspring, increased calving interval and reduction of milk yield and it is endemic in Bangladesh. In this study we collected milk and blood samples simultaneously from533 cows of Central Cattle Breeding and Dairy Farm, Savar, Dhaka and different Upazilas of Gaibandha and Mymensingh District. Five hundred thirty three samples were examined for antibodies to Brucella using the Milk Ring Test (MRT) and Rose Bengal Test (RBT). Overall 2.62 % of milk samples were positive according to MRT, while2.06 % of the serum samples were positive to the RBT. Only 6 (1.13 %) of the samples were positive for both test (MRT and RBT). Out of 312 samples only 10 (3.20 %) were positive to MRT while 8(2.06%) were positive to RBT in Holstein Friesian cross (p>0.05) on the other hand out of 221 samples only 4 (1.80%) were positive to MRT while 3(1.35%) were positive to RBT in Sahiwal cross. The prevalence of brucellosis was significantly higher in the age group of > 5 years than other age groups (p?0.01) on both test (MRT 2.75%and RBT 2.25%).Based on parity, significantly higher prevalence (MRT 2.93% and RBT 2.44%) of MRT and RBT were obtainedin parity 3-5in comparison to other parity group (p?0.01). It is, however, obvious that although the MRT is 1st-line screening tests for brucellosis in cows in some countries, their lack of specificity is of concern. Therefore, the requirement for other confirmatory tests that are more specific should be used for the diagnosis of the disease, especially in Bangladesh.DOI: http://dx.doi.org/10.3329/bjvm.v12i2.21280 Bangl. J. Vet. Med. (2014). 12 (2): 167-171 

scholarly journals Foreign Body Aspiration in Infants and Older Children: A Comparative Study

2019 ◽  
Vol 99 (1) ◽  
pp. 47-51 ◽  
Author(s):  
Shorook Na’ara ◽  
Igor Vainer ◽  
Moran Amit ◽  
Arie Gordin
Keyword(s):  
Foreign Body ◽  
Medical Center ◽  
Age Groups ◽  
Signs And Symptoms ◽  
Foreign Body Aspiration ◽  
Preschool Age ◽  
Small Airways ◽  
Older Children ◽  
X Ray ◽  
Young Infants

Background: Foreign body aspiration (FBA) is a major cause of morbidity and mortality in children. It is a preventable event that predominates in preschool age. The signs and symptoms mimic respiratory diseases common in the same age-group. We compared FBA in infants to FBA in older children. Methods: Retrospective analysis of all the cases of suspected FBA of children under the age of 18 years hospitalized at one medical center during 2002 to 2016. We analyzed the data according to age: up to 1 year (infants) and 1 to 18 years. Results: One hundred seventy-five children with suspected FBA were admitted; of whom, 27 (15%) were infants and 148 (85%) were older children (age 1-18 years). For the 2 age groups, adults witnessed 85% and 73%, respectively, of the incidents ( P = .4). In the neonate group, 48% presented with normal X-ray findings compared to only 20% in the older group; 15% of the older group had a positive chest X-ray for a foreign body, while none had such in the infants’ group ( P = .01). For the 2 age groups, the majority of the FBs found were from organic origin. About half of the patients were diagnosed and managed within 24 hours of the aspiration event. In 10%, repeated bronchoscopy was performed due to a retained FB remnant. In a multivariate analysis, signs and symptoms ( P < .05), location of the FB ( P < .001), and witnessed aspiration ( P < .001) were independent prognostic factors for the length of hospitalization. Conclusion: Foreign body aspiration is not uncommon in young infants; the management is challenging due to small airways, the need to use smaller bronchoscopes, and the lack of working channel forces in pediatric bronchoscopes.

scholarly journals Investigation of Epstein-Barr Virus Antibodies by Chemiluminescent Microparticle Immunoassay Method in Patients of Different Age Groups: Evaluation of Atypical Serological Profiles

2021 ◽  
pp. 1-6
Author(s):  
Alev Çetin Duran ◽  
Tuğba Kula Ati
Keyword(s):  
Epstein Barr Virus ◽  
Acute Infection ◽  
Age Groups ◽  
Igg Antibodies ◽  
Serological Tests ◽  
Past Infection ◽  
Barr Virus ◽  
Serological Profile ◽  
Chemiluminescent Microparticle Immunoassay ◽  
Epstein Barr

Objective: Epstein-Barr virus (EBV) can cause different clinical pictures from infectious mononucleosis (IM) to malignancies such as B-cell lymphomas, Burkitt’s lymphoma, nasopharyngeal carcinoma, and Hodgkin lymphoma. VCA-IgM, VCA-IgG, EBNA-1 IgG antibodies are the most commonly used antibodies in revealing the serological profile. This study aimed to examine the serological profiles of patients with suspected EBV infection and to interpret the atypical profiles encountered. Methods: The results of VCA-IgM, VCA-IgG, and EBNA-1 IgG antibodies studied in the Microbiology Laboratory between 2017-2019 were evaluated retrospectively. EBV serological tests (VCA-IgM, VCA-IgG, and EBNA-1 IgG) were performed according to the manufacturer’s recommendations using the chemiluminescent microparticle immunoassay (CMIA) method (Architect, Abbott, Wiesbaden, Germany). Results: Of the 2486 patients evaluated, 1341 (53.9%) were male, 1145 (46.1%) were female, and the average age was determined as 16.93 ± 19.5. EBV past infection was detected in 56.65% of the cases, the acute infection was detected in 17.25%, and 21.09% did not encounter EBV. Atypical serological profile was detected in 5.01%. As an atypical profile, the most common positivity of three antibodies together (3.90%), then isolated VCA-IgG positivity (0.91%), and isolated EBNA-1 IgG positivity (0.20%) were determined. It was determined that 24.24% of the cases with an atypical profile were immunosuppressive patient.  Conclusions: The rate of encountering EBV in our study is 78.91%. The atypical EBV serological profile rate was found to be 5.01%. Approximately one-fourth of the cases with an atypical profile was found to be in the patient group with immune disorders. It is thought that antibody tests are not sufficient to determine the stage of infection, especially in these patient groups, and further tests should be performed. It has been demonstrated that serological monitoring is required for the interpretation of atypical profiles. Keywords: Epstein-Barr virüs, serological tests, chemiluminescent microparticle immunoassay (CMIA), atypical serological profile

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