Developmental Distribution of Microperoxisomes in the Rat Submandibular Gland

1977 ◽  
Vol 35 ◽  
pp. 426-427
Author(s):  
B.A. Mooradian ◽  
L.S. Cutler
Keyword(s):  
Postnatal Development ◽  
Submandibular Gland ◽  
Cell Types ◽  
Functional Differentiation ◽  
Secretory Cells ◽  
Ductal Cells ◽  
Size Number ◽  
Rat Submandibular Gland

In the mature rat submandibular gland (SMG) microperoxisomes have been identified in both acinar secretory cells and ductal cells. The present study was undertaken to investigate the size, number and distribution of microperoxisomes during the pre and postnatal development of the SMG in order to determine if there were changes in cellular microperoxisomes during the functional differentiation of the various cell types.

scholarly journals Developmental distribution of microperoxisomes in the rat submandibular gland.

1978 ◽  
Vol 26 (11) ◽  
pp. 989-999 ◽  
Author(s):  
B A Mooradian ◽  
L S Cutler
Keyword(s):  
Postnatal Development ◽  
Submandibular Gland ◽  
Acinar Cell ◽  
Secretory Cell ◽  
Fold Increase ◽  
Duct Cells ◽  
Size Number ◽  
Tubule Cells ◽  
Prenatal And Postnatal Development ◽  
Rat Submandibular Gland

The present study investigated the size, number, and distribution of microperoxisomes (MP) during the prenatal and postnatal development of the rat submandibular gland (SMG). A three-fold increase in MP number per cell was observed in the cells of the rudiment from the 15th to the 16th day of gestation. The early secretory and striated duct cells contained about 9.0 MP. The number of MP per secretory cell decreased such that 3.5 MP were found in each mature acinar cell. In the striated duct cells, MP number progressively increased to 40.0. As the convoluted granular tubule cells (CGT) developed from striated duct cells there was an increase in MP number from 16.0 to 26.0/cell. At maturity, the convoluted granular tubule cells contained only 14.0 MP. Throughout development of the SMG, intercalated duct cells showed only rare MP. The data suggests that the number, size, and distribution of MP changes as a function of the particular path of differentiation followed by the various cells in the rat SMG.

Intercellular contacts at the epithelial—mesenchymal interface of the developing rat submandibular gland in vitro

Development ◽  
1977 ◽  
Vol 39 (1) ◽  
pp. 71-77
Author(s):  
Leslie S. Cutler
Keyword(s):  
Electron Microscopy ◽  
Submandibular Gland ◽  
Functional Differentiation ◽  
Developmental Sequence ◽  
Millipore Filter ◽  
Intercellular Contacts ◽  
Rat Submandibular Gland ◽  
Developing Rat

An ultrastructural study of the development of the rat submandibular gland (SMG) anlage in vitro was undertaken to determine if epithelial-mesenchymal and epithelial-nerve contacts were integral events in the differentiation of the gland in vitro as they are in vivo. SMG rudiments were removed at the stalk-bulb stage (15 days in utero) and cultured for 6 days on a millipore filter in supplemented McCoy's 5A media. Rudiments were taken at daily intervals, fixed and processed for electron microscopy. The overall development of the explanted rudiments closely paralleled their maturation in vivo although cultured glands lagged 24–36 h behind their normal counterparts. Direct epithelial-mesenchymal contacts were seen after the morphogenetic patterning of the gland had been established but prior to functional differentiation of the rudiment. Epithelial-nerve contacts were not seen although healthy axons were seen in the stroma throughout the culture period. The study indicates that epithelial-nerve contacts are probably not required for morphogenesis of cytodifferentiation of the rat SMG. However, direct epithelial-mesenchymal contacts appear to be an integral part of the developmental sequence of the rat SMG.

scholarly journals Diverse Epithelial Cell Populations Contribute to Regeneration of Secretory Units in Injured Salivary Glands

2020 ◽  
Author(s):  
Ninche Ninche ◽  
Mingyu Kwak ◽  
Soosan Ghazizadeh
Keyword(s):  
Stem Cells ◽  
Salivary Glands ◽  
De Novo ◽  
Cell Types ◽  
Myoepithelial Cells ◽  
Lineage Tracing ◽  
Secretory Cells ◽  
Cell Populations ◽  
Cellular Plasticity ◽  
Ductal Cells

ABSTRACTSalivary glands exert exocrine secretory function to provide saliva for lubrication and protection of the oral cavity. Its epithelium consists of several differentiated cell types including acinar, ductal and myoepithelial cells that are maintained in a lineage-restricted manner during homeostasis or after mild injuries. Glandular regeneration following a near complete loss of secretory cells, however, may involve cellular plasticity, although the mechanism and extent of such plasticity remain unclear. Here, by combining lineage-tracing experiments with a model of severe glandular injury in the mouse submandibular gland, we show that de novo formation of secretory units involves induction of cellular plasticity in multiple non-acinar cell populations. Fate-mapping analysis revealed that although ductal stem cells marked by cytokeratin K14 and Axin2 undergo a multipotency switch, they do not make a significant contribution to acinar regeneration. Intriguingly, more than 80% of regenerated acini derive from differentiated cells including myoepithelial and ductal cells that dedifferentiate to a progenitor-like state before redifferentiation to acinar cells. The potential of diverse cell populations serving as a reserve source for acini widens the therapeutic options for hyposalivation.SummarySalivary glands rely in recruitment of committed and fully differentiated cell populations as well as stem cells to ensure rapid regeneration and recovery of secretory cells.

scholarly journals Immunohistochemical localization of Na+,K+-ATPase in rodent and human salivary and lacrimal glands.

1988 ◽  
Vol 36 (9) ◽  
pp. 1139-1145 ◽  
Author(s):  
D C Winston ◽  
R A Hennigar ◽  
S S Spicer ◽  
J R Garrett ◽  
B A Schulte
Keyword(s):  
Submandibular Gland ◽  
Immunohistochemical Localization ◽  
Secretory Cells ◽  
Mucous Cells ◽  
Duct System ◽  
Lacrimal Glands ◽  
Low Protein ◽  
Rats And Mice ◽  
Rat Submandibular Gland ◽  
Gland Type

The enzyme Na+,K+-ATPase was localized immunohistochemically in major salivary glands of mouse, rat, and human and in exorbital lacrimal glands of the rodents. Immunoreactive Na+,K+-ATPase was abundant in the basolateral membranes of all epithelial cells lining striated and intra- and interlobular ducts of all glands. Reactivity of intercalated ducts varied among gland type and species. Cells lining granular ducts in rodent submandibular gland showed a heterogeneous staining pattern in rat but stained homogeneously in mouse. Secretory cells varied greatly in their content of immunoreactive Na+,K+-ATPase. As with all duct cells, staining was present only at the basolateral surface and was never observed at the luminal surface of reactive secretory cells. Mucous cells failed to show any reactivity in any gland examined. Serous cells showed a gradient of immunostaining intensity ranging from strongly positive in demilunes of human sublingual gland to negative in rat submandibular gland and lacrimal glands of rats and mice. The presence of basolaterally localized Na+,K+-ATPase in most serous cells but not in mucous cells suggests that the enzyme contributes to the ion and water content of copious, low-protein serous secretions. The intense immunostaining of cells in most if not all segments of the duct system supports the idea that the ducts are involved with modification of the primary saliva, and extends this concept to include all segments of the duct system.

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