Cross-sectional serosurvey of Coxiella burnetii in healthy cattle and sheep from extensive grazing system in central Italy

Abstract A cross-sectional survey was carried out to estimate the seroprevalence of Coxiella burnetii in extensively grazed cattle and sheep from central Italy and to identify the related risk factors. Data on notified human Q fever cases in the area were also collected and described. A two-stage cluster sampling was performed. A total of 5083 animals (2210 cattle; 2873 sheep) belonging to 186 farms (92 herds; 94 flocks) were tested for the presence of antibodies against C. burnetii using a commercial enzyme-linked immunosorbent assay kit. The prevalence at the animal-level resulted three times higher in sheep compared to cattle (37.8% vs. 12.0%; χ2 = 270.10, P < 0.001). The prevalence at the herd-level was also higher in sheep than in cattle (87.2% vs. 68.5%; χ2 = 9.52, P < 0.01). The multivariate analysis showed a higher risk of seropositivity for cattle aged 67–107 months (OR 2.79, 95% CI 1.86–4.18), cattle >107 months of age (OR 2.07, 95% CI 1.36–3.14) and mixed breed cattle (OR 1.74, 95% CI 1.11–2.72). A herd size >92 animals was recognized as herd-level risk factor in cattle (OR 6.88, 95% CI 1.67–28.37). The risk of being seropositive was double in sheep belonging to flocks >600 animals (odds ratio (OR) 2.04, 95% CI 1.63–2.56). Sheep were confirmed to be the most exposed species. Nevertheless, the prevalence observed in cattle also suggests the potential involvement of this species in the circulation of the pathogen in the area. Seven confirmed human Q fever cases were reported. In five out of seven cases there was at least one exposed herd within a 5 km buffer. Even though the source of the infection was not identified, the possibility of C. burnetii circulating in the livestock and human population in the study area cannot be overlooked. The integration between veterinary and human surveillance will be crucial to understand the spread of this zoonosis and to support the adoption of appropriate control measures.

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Seroprevalence of Coxiella burnetii in sheep flocks in Kaduna State, Northwestern Nigeria

Acta Veterinaria Hungarica ◽

10.1556/004.2021.00034 ◽

2021 ◽

Author(s):

Shuaibu Gidado Adamu ◽

Junaidu Kabir ◽

Jarlath Udo Umoh ◽

Mashood Abiola Raji

Keyword(s):

Risk Factors ◽

Coxiella Burnetii ◽

Q Fever ◽

Jugular Vein ◽

Cross Sectional Study ◽

Enzyme Linked Immunosorbent Assay ◽

Sectional Study ◽

High Seroprevalence ◽

Cross Sectional ◽

Significant Difference

Abstract A cross-sectional study was carried out to determine the seroprevalence and risk factors of Q fever in sheep in the northern part of Kaduna State, Nigeria. This study aimed to determine Coxiella burnetii infection and its risk factors in sheep in Kaduna State. A total of 400 blood samples consisting of 259 samples from females and 141 from males were aseptically collected from the jugular vein of sheep from flocks in Kaduna State. The sera obtained were screened for Q fever using an indirect enzyme-linked immunosorbent assay (iELISA). The obtained data were analysed to determine whether there is a relationship between sex, age, and the animals tested. The analysis revealed that 8.0% of the sera was seropositive by iELISA. There was no significant difference in Q fever seropositivity in the study area according to the sex of sheep (P > 0.05). There was a statistically significant difference (P < 0.05) in Q fever seropositivity according to the age of sheep. This study indicated a high seroprevalence of Q fever mainly among female animals and older sheep. Further studies are required to determine the epizootiology of Q fever in the study area more precisely.

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Risk factors for an infection with Coxiella burnetii in German sheep flocks

Epidemiology and Infection ◽

10.1017/s0950268820002447 ◽

2020 ◽

Vol 148 ◽

Author(s):

A. Wolf ◽

T. L. Prüfer ◽

C. Schoneberg ◽

A. Campe ◽

M. Runge ◽

...

Keyword(s):

Risk Factors ◽

Coxiella Burnetii ◽

Q Fever ◽

Small Ruminants ◽

Multivariable Logistic Regression Analysis ◽

Specific Gene ◽

Adult Males ◽

Cross Sectional ◽

Sheep Population ◽

Herd Level

Abstract In Germany, sheep are the main source of human Q fever epidemics, but data on Coxiella burnetii (C. burnetii) infections and related risk factors in the German sheep population remain scarce. In this cross-sectional study, a standardised interview was conducted across 71 exclusively sheep as well as mixed (sheep and goat) farms to identify animal and herd level risk factors associated with the detection of C. burnetii antibodies or pathogen-specific gene fragments via univariable and multivariable logistic regression analysis. Serum samples and genital swabs from adult males and females of 3367 small ruminants from 71 farms were collected and analysed using ELISA and qPCR, respectively. On animal level, univariable analysis identified young animals (<2 years of age; odds ratio (OR) 0.33; 95% confidence interval (CI) 0.13–0.83) to reduce the risk for seropositivity significantly (p < 0.05). The final multivariable logistic models identified lambing all year-round (OR 3.46/3.65; 95% CI 0.80–15.06/0.41–32.06) and purchases of sheep and goats (OR 13.61/22.99; 95% CI 2.86–64.64/2.21–239.42) as risk factors on herd level for C. burnetii infection detected via ELISA and qPCR, respectively.

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Prevalence and Factors Associated With Burnout of Frontline Healthcare Workers in Fighting Against the COVID-19 Pandemic: Evidence From China

Frontiers in Psychology ◽

10.3389/fpsyg.2021.680614 ◽

2021 ◽

Vol 12 ◽

Author(s):

Xin Zhang ◽

Jiahui Wang ◽

Yanhua Hao ◽

Ke Wu ◽

Mingli Jiao ◽

...

Keyword(s):

Infection Control ◽

Healthcare Workers ◽

Final Analysis ◽

Maslach Burnout Inventory ◽

Control Measures ◽

Cluster Sampling ◽

Cross Sectional Survey ◽

Cross Sectional ◽

Factors Associated ◽

Infection Control Measures

ObjectivesThe sudden outbreak of the novel coronavirus disease (COVID-19) plunged healthcare workers (HCWs) into warfare. This study aimed to determine the prevalence of burnout and the factors associated with it among frontline HCWs fighting COVID-19.MethodsA cross-sectional survey was conducted among frontline HCWs fighting against the COVID-19 in Wuhan, Harbin, and Shenzhen during the period from February 18 to March 4. Finally, HCWs were recruited using cluster sampling, 1,163 HCWs were included in the final analysis. Burnout was measured using a 22-item Maslach Burnout Inventory scale (MBI scale).ResultsOf the participants, 48.6% suffered from burnout, and 21.8% showed a high degree of burnout. Doctors (b = 3.954, P = 0.011) and nurses (b = 3.067, P = 0.042) showed higher emotional exhaustion (EE) than administrators. Participants who worked continuously for more than 8 h a day (b = 3.392, P = 0.000), those who were unable to eat three regular daily meals (b = 2.225, P = 0.008), whose daily water intake was no more than 800 ml (b = 3.007, P = 0.000), who slept for no more than 6 h (b = 1.609, P = 0.036), and who were infected or had colleagues who were infected with COVID-19 (b = 4.182, P = 0.000) experienced much higher levels of EE, while those who could adhere to infection control procedures (b = −5.992, P = 0.000), who were satisfied with their hospital’s infection control measures(b = −3.709, P = 0.001), and who could receive sufficient psychological crisis intervention (b = −1.588, P = 0.039) reported lower levels of EE.ConclusionThe study reveals that burnout is prevalent among frontline HCWs and that the known factors associated with burnout, such as workload, and the factors directly associated with COVID-19, such as having insufficient protection, can affect burnout symptoms in frontline HCWs. Synergized and comprehensive interventions should be targeted at reducing its occurrence among frontline HCWs fighting COVID-19.

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890The prevalence and risk factors for Coxiella burnetii on commercial dairy goat farms in Australia

International Journal of Epidemiology ◽

10.1093/ije/dyab168.289 ◽

2021 ◽

Vol 50 (Supplement_1) ◽

Author(s):

Kangwei Hou ◽

Simon Firestone ◽

Anke Wiethoelter ◽

John Stenos ◽

Louis Lignereux ◽

...

Keyword(s):

Risk Factors ◽

Coxiella Burnetii ◽

Q Fever ◽

Quantitative Polymerase Chain Reaction ◽

Dairy Goat ◽

Enzyme Linked Immunosorbent Assay ◽

Rt Pcr ◽

Dairy Goats ◽

Cross Sectional ◽

Pcr Targeting

Abstract Background Despite the potentially important role that intensively managed dairy goats play in the spread of Q fever, the prevalence of Coxiella burnetii among dairy goat herds in Australia is largely unknown. The aim of this cross-sectional study was to estimate the prevalence of coxiellosis-positive dairy goat herds in Australia and to identify risk factors associated with coxiellosis positivity. Methods Owners or managers of commercial dairy goat herds were contacted and asked to complete a questionnaire about risk factors for coxiellosis and to provide a bulk tank milk (BTM) sample. BTM samples were tested using an enzyme-linked immunosorbent assay (ELISA) and real-time quantitative polymerase chain reaction (RT-PCR) targeting the Com1 and IS1111 sections of the C. burnetii genome. Questionnaire responses from coxiellosis positive and coxiellosis negative herds were compared using frequency cross-tabulations and multivariable logistic regression. Results Herd managers from 49 of the 61 commercial dairy goat herds in Australia took part in the study. Of this group, three BTM samples were found to be both ELISA and RT-PCR positive. Two BTM samples were ELISA positive but RT-PCR negative. There were 10 (95% CI 4.4 to 22) C. burnetii positive herds per 100 herds at risk. Conclusions The prevalence of coxiellosis among commercial dairy goat farms in Australia is relatively low. Key messages The Australian dairy goat industry should focus on biosecurity measures and risk management plans to reduce the probability of C. burnetii introduction.

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Malaria Prevention and Mothers: Sudan

Basic and Applied Sciences - Scientific Journal of King Faisal University ◽

10.37575/b/med/2384 ◽

2021 ◽

Author(s):

Yousif Elmosaad ◽

Ahmed Al Rajeh ◽

Asif khan ◽

Elfatih Malik ◽

Ilias Mahmud

Keyword(s):

Formal Education ◽

Effective Means ◽

Malaria Prevention ◽

Good Knowledge ◽

Bed Nets ◽

Cluster Sampling ◽

Cross Sectional Survey ◽

Cross Sectional ◽

Attitudes And Practices ◽

Positive Attitudes

This study assessed the knowledge, attitudes, and practices (KAP) in malaria prevention using insecticide-treated bed nets (ITNs) among mothers of children under five years of age in White Nile State, Sudan. Multistage cluster sampling was used to select 761 mothers for this cross-sectional survey. There were gaps in the KAP in malaria prevention. Only 46.3% of the mothers stated personal protective measures (PPMs) as the best malaria prevention strategy; 54.9% considered ITNs as an effective means; and only 18.7% reported sleeping under an ITN every day. Older mothers were less likely to have good knowledge (OR=0.96), attitudes (OR=0.98) and practices (OR=0.98). Having a Government employee as the head of the household was positively associated with knowledge (OR=2.16) and attitudes (OR=1.96). The mother having a formal education was also positively associated with good knowledge (OR=1.55) and positive attitudes (OR=1.69). Mothers with a monthly household income of <491 Sudanese Pound were more likely to have good knowledge (OR=1.43). Mothers who had good knowledge (OR=4.99) and positive attitudes towards PPMs (OR=2.60) in malaria prevention were found to be more likely to practice preventive measures. Therefore, we recommend focusing on raising mothers’ awareness of the different preventive activities to protect them and their family from malaria.

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Validation of a Novel Commercial ELISA Test for the Detection of Antibodies against Coxiella burnetii

Pathogens ◽

10.3390/pathogens9121075 ◽

2020 ◽

Vol 9 (12) ◽

pp. 1075

Author(s):

Salvatore Ledda ◽

Cinzia Santucciu ◽

Valentina Chisu ◽

Giovanna Masala

Keyword(s):

Diagnostic Accuracy ◽

Phase Ii ◽

Coxiella Burnetii ◽

Q Fever ◽

Animal Health ◽

Elisa Test ◽

Clinical Diagnostics ◽

Complex Life Cycle ◽

Enzyme Linked Immunosorbent Assay ◽

Specificity And Sensitivity

Q fever is a zoonosis caused by Coxiella burnetii, a Gram-negative pathogen with a complex life cycle and a high impact on public and animal health all over the world. The symptoms are indistinguishable from those belonging to other diseases, and the disease could be symptomless. For these reasons, reliable laboratory tests are essential for an accurate diagnosis. The aim of this study was to validate a novel enzyme-linked immunosorbent assay (ELISA) test, named the Chorus Q Fever Phase II IgG and IgM Kit (DIESSE, Diagnostica Senese S.p.A), which is performed by an instrument named Chorus, a new device in medical diagnostics. This diagnostic test is employed for the detection of antibodies against C. burnetii Phase II antigens in acute disease. Our validation protocol was performed according to the Italian Accreditation Body (ACCREDIA) (Regulation UNI CEI EN ISO/IEC 17025:2018 and 17043:2010), OIE (World Organization for Animal Health), and Statement for Reporting Studies of Diagnostic Accuracy (STARD). Operator performance was evaluated along with the analytical specificity and sensitivity (ASp and ASe) and diagnostic accuracy of the kit, with parameters such as diagnostic specificity and sensitivity (DSp and DSe) and positive and negative predictive values (PPV and NPV), in addition to the repeatability. According to the evaluated parameters, the diagnostic ELISA test was shown to be suitable for validation and commercialization as a screening method in human sera and a valid support for clinical diagnostics.

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Peste des Petits Ruminants Virus Infection at the Wildlife–Livestock Interface in the Greater Serengeti Ecosystem, 2015–2019

Viruses ◽

10.3390/v13050838 ◽

2021 ◽

Vol 13 (5) ◽

pp. 838

Author(s):

Bryony A. Jones ◽

Mana Mahapatra ◽

Daniel Mdetele ◽

Julius Keyyu ◽

Francis Gakuya ◽

...

Keyword(s):

Small Ruminants ◽

Viral Disease ◽

Global Strategy ◽

Enzyme Linked Immunosorbent Assay ◽

Weighted Estimate ◽

Peste Des Petits Ruminants ◽

African Buffalo ◽

Cross Sectional Survey ◽

Cross Sectional ◽

Serengeti Ecosystem

Peste des petits ruminants (PPR) is a viral disease of goats and sheep that occurs in Africa, the Middle East and Asia with a severe impact on livelihoods and livestock trade. Many wild artiodactyls are susceptible to PPR virus (PPRV) infection, and some outbreaks have threatened endangered wild populations. The role of wild species in PPRV epidemiology is unclear, which is a knowledge gap for the Global Strategy for the Control and Eradication of PPR. These studies aimed to investigate PPRV infection in wild artiodactyls in the Greater Serengeti and Amboseli ecosystems of Kenya and Tanzania. Out of 132 animals purposively sampled in 2015–2016, 19.7% were PPRV seropositive by ID Screen PPR competition enzyme-linked immunosorbent assay (cELISA; IDvet, France) from the following species: African buffalo, wildebeest, topi, kongoni, Grant’s gazelle, impala, Thomson’s gazelle, warthog and gerenuk, while waterbuck and lesser kudu were seronegative. In 2018–2019, a cross-sectional survey of randomly selected African buffalo and Grant’s gazelle herds was conducted. The weighted estimate of PPRV seroprevalence was 12.0% out of 191 African buffalo and 1.1% out of 139 Grant’s gazelles. All ocular and nasal swabs and faeces were negative by PPRV real-time reverse transcription-polymerase chain reaction (RT-qPCR). Investigations of a PPR-like disease in sheep and goats confirmed PPRV circulation in the area by rapid detection test and/or RT-qPCR. These results demonstrated serological evidence of PPRV infection in wild artiodactyl species at the wildlife–livestock interface in this ecosystem where PPRV is endemic in domestic small ruminants. Exposure to PPRV could be via spillover from infected small ruminants or from transmission between wild animals, while the relatively low seroprevalence suggests that sustained transmission is unlikely. Further studies of other major wild artiodactyls in this ecosystem are required, such as impala, Thomson’s gazelle and wildebeest.

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Analyzing Predictors of Control Measures and Psychosocial Problems Associated with COVID-19 Pandemic: Evidence from Eight Countries

Behavioral Sciences ◽

10.3390/bs11080106 ◽

2021 ◽

Vol 11 (8) ◽

pp. 106

Author(s):

Sheikh Saifur Rahman Jony ◽

Ubydul Haque ◽

Nathaniel J. Webb ◽

Emily Spence ◽

Md. Siddikur Rahman ◽

...

Keyword(s):

Psychological Health ◽

Age Groups ◽

Psychosocial Problems ◽

Relevant Information ◽

The United States ◽

Control Measures ◽

Cross Sectional Survey ◽

Online Questionnaire ◽

Cross Sectional ◽

Primary Determinant

COVID-19 has harshly impacted communities globally. This study provides relevant information for creating equitable policy interventions to combat the spread of COVID-19. This study aims to predict the knowledge, attitude, and practice (KAP) of the COVID-19 pandemic at a global level to determine control measures and psychosocial problems. A cross-sectional survey was conducted from July to October 2020 using an online questionnaire. Questionnaires were initially distributed to academicians worldwide. These participants distributed the survey among their social, professional, and personal groups. Responses were collected and analyzed from 67 countries, with a sample size of 3031. Finally, based on the number of respondents, eight countries, including Bangladesh, China, Japan, Malaysia, Mexico, Pakistan, the United States, and Zambia were rigorously analyzed. Specifically, questionnaire responses related to COVID-19 accessibility, behavior, knowledge, opinion, psychological health, and susceptibility were collected and analyzed. As per our analysis, age groups were found to be a primary determinant of behavior, knowledge, opinion, psychological health, and susceptibility scores. Gender was the second most influential determinant for all metrics except information about COVID-19 accessibility, for which education was the second most important determinant. Respondent profession was the third most important metric for all scores. Our findings suggest that greater encouragement from government health authorities and the promotion of health education and policies are essential in the dissemination of COVID-19-awareness and increased control of the spread of COVID-19.

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Seroprevalence of Toxoplasma gondii and associated risk factors in domestic pigs raised from Cuba

Parasitology Research ◽

10.1007/s00436-021-07245-1 ◽

2021 ◽

Author(s):

Julio César Castillo-Cuenca ◽

Álvaro Martínez-Moreno ◽

José Manuel Diaz-Cao ◽

Angel Entrena-García ◽

Jorge Fraga ◽

...

Keyword(s):

Risk Factors ◽

Toxoplasma Gondii ◽

Meat Products ◽

Control Measures ◽

Health Concern ◽

Enzyme Linked Immunosorbent Assay ◽

Serum Samples ◽

Cross Sectional ◽

Weaning Pigs ◽

Associated Risk Factors

AbstractA cross-sectional study was carried out to determine the seroprevalence of Toxoplasma gondii and associated risk factors in pigs in the largest pork-producing region in Cuba. Serum samples from 420 pigs, including 210 sows and 210 post-weaning pigs, were tested for antibodies against T. gondii using a commercial indirect enzyme-linked immunosorbent assay. Anti-T. gondii antibodies were detected in 56 animals (13.3%, 95% CI: 10.1–16.6). A generalized estimating equations model revealed that the risk factors associated with higher seropositivity in pigs were altitude (higher in farm’s location < 250 m above sea level (masl) versus ≥ 250 masl) and age (higher in sows compared to post-weaning pigs). The results indicated that this protozoan parasite is widely distributed on pig farms in the study area, which is a public health concern since the consumption of raw or undercooked pork meat products containing tissue cysts is considered one of the main routes of T. gondii transmission worldwide. Control measures should be implemented to reduce the risk of exposure to T. gondii in pigs in Cuba.

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Child Nutrition Among Circular Migrant Families Working in the Brick Kilns of Bihar, India (P10-052-19)

Current Developments in Nutrition ◽

10.1093/cdn/nzz034.p10-052-19 ◽

2019 ◽

Vol 3 (Supplement_1) ◽

Author(s):

Reshma Roshania ◽

Rakesh Giri ◽

Melissa Fox Young ◽

G Sai Mala ◽

Amy Webb Girard ◽

...

Keyword(s):

Immunization Coverage ◽

Wealth Index ◽

Household Wealth ◽

Nutrition Status ◽

Cluster Sampling ◽

Z Score ◽

Cross Sectional Survey ◽

Cross Sectional ◽

Brick Kilns ◽

Migrant Families

Abstract Objectives The objective of this study was to estimate the prevalence of child malnutrition and the determinants of nutrition status among circular migrant families working in the brick industry in Bihar, India, focusing on differences by origin. Methods We used a stratified, cluster sampling design consisting of a cross-sectional survey in 552 randomly selected brick kilns (clusters) throughout Bihar. Circular migration was defined as residence outside the home block for at least 60 days for employment plus at least one home return in the previous year. Per kiln, three circular migrant households with children 0–35 months of age were randomly selected. We collected kiln, household and child-level data including anthropometric measurements for each selected child (n = 1198). Descriptive, bivariate and logistic regression analyses were conducted in SAS. The primary outcomes were stunting (<-2 SD height-for-age z score) and wasting (<-2 SD weight-for-height z score). The primary exposure of interest was intrastate vs. interstate origin; covariates included household wealth index, parity of the mother, and child age and gender. Results Prevalence of stunting was lower among interstate migrants (47%) compared to intrastate migrants (55%, aOR: 0.66, 95%CI: 0.50–0.88). Wasting was higher among interstate migrants (43%) compared to intrastate migrants (34%, aOR:1.51, 95%CI: 1.17–1.94). Among children 6–23 months, 13% had a minimum acceptable diet; MAD was higher among interstate migrants (17%), compared to intrastate migrants (10%) (P = 0.014). Full immunization coverage among circular migrants was 39%, lower than the overall state (62%, NFHS IV). Open defecation was reported by over 90% of circular migrants. Conclusions Our results are likely to inform the ongoing policy discourse on circular migrants’ access to health and nutrition benefits. State of origin emerged as an important predictor of nutrition status, operating differently for acute and chronic malnutrition; we will further explore pathways of nutrition by origin in future analyses. Funding Sources Bill & Melinda Gates Foundation.

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