scholarly journals Using seed respiration as a tool for calculating optimal soaking times for ‘on-farm’ seed priming of barley (Hordeum vulgare)

2021 ◽  
pp. 1-9
Author(s):  
Javier Carrillo-Reche ◽  
Adrian C. Newton ◽  
Richard S. Quilliam
Keyword(s):  
Morphological Changes ◽  
Low Cost ◽  
Germination Rate ◽  
Seed Priming ◽  
Embryonic Axes ◽  
Seedling Vigour ◽  
Soaking Time ◽  
On Farm ◽  
Seed Respiration

Abstract A low-cost technique named ‘on-farm’ seed priming is increasingly being recognized as an effective approach to maximize crop establishment. It consists of anaerobically soaking seeds in water before sowing resulting in rapid and uniform germination, and enhanced seedling vigour. The extent of these benefits depends on the soaking time. The current determination of optimal soaking time by germination assays and mini-plot trials is resource-intensive, as it is species/genotype-specific. This study aimed to determine the potential of the seed respiration rate (an indicator of metabolic activity) and seed morphological changes during barley priming as predictors of the priming benefits and, thus, facilitate the determination of optimal soaking times. A series of germination tests revealed that the germination rate is mostly attributable to the rapid hydration of embryo tissues, as the highest gains in the germination rate occurred before the resumption of respiration. Germination uniformity, however, was not significantly improved until seeds were primed for at least 8 h, that is, after a first respiration burst was initiated. The maximum seedling vigour was attained when the priming was stopped just before the beginning of the differentiation of embryonic axes (20 h) after which vigour began to decrease (‘over-priming’). The onset of embryonic axis elongation was preceded by a second respiration burst, which can be used as a marker for priming optimization. Thus, monitoring of seed respiration provides a rapid and inexpensive alternative to the current practice. The method could be carried out by agricultural institutions to provide recommended optimal soaking times for the common barley varieties within a specific region.

scholarly journals EFFECT OF PRIMING WITH SILVER FIR AND OREGANO ESSENTIAL OILS ON SEED GERMINATION AND VIGOUR OF SILENE SENDTNERI

2021 ◽  
Vol 50 (1) ◽  
pp. 27-32
Author(s):  
Erna Karalija ◽  
Fatima Pustahija ◽  
Adisa Parić
Keyword(s):  
Essential Oils ◽  
Water Content ◽  
Germination Rate ◽  
Photosynthetic Pigment ◽  
Dry Weight ◽  
Seed Priming ◽  
Silver Fir ◽  
Seedling Vigour ◽  
Seed Vigour ◽  
Oregano Oil

UDK: 582.661.51:631.53.027]:547.913 In order to investigate the effects of seed priming with silver fir and oregano essential oils on certain important seedling characteristic and seed vigour of interesting endemic and horticultural species Silene sendtneri, an experiment was conducted based on randomized completely design with three replications. Traits such as germination rate, water content, dry weight, vigour index and photosynthetic pigments were analysed. Results revealed that the seedlings obtained with priming seeds showed increased growth, water content, vigour and photosynthetic pigment contents but decreased germination rate and dry weight compared with that obtained with non-primed seeds. Analysis of seed priming effects had demonstrated even germination rate is smaller the seedling vigour is slightly higher especially with all oregano oil treatments. We suggest that oregano oil has a potential as a priming agent for improvement of seedling synchrony, although at lower rate of germination.

The improvement of crop yield in marginal environments using 'on-farm' seed priming: nodulation, nitrogen fixation and disease resisitance

2005 ◽  
Vol 56 (11) ◽  
pp. 1211 ◽  
Author(s):  
D. Harris ◽  
W. A. Breese ◽  
J. V. D. K. Kumar Rao
Keyword(s):  
Field Experiments ◽  
Low Cost ◽  
Seed Priming ◽  
Plant Performance ◽  
Individual Plant ◽  
Rhizobium Inoculation ◽  
Marginal Areas ◽  
Resource Poor ◽  
On Farm

On-farm seed priming with water is a low-cost, low-risk technology that is easily adopted by resource-poor farmers. It increases the yield of tropical and subtropical annual crops in marginal areas by a combination of better crop establishment and improved individual plant performance. The effects of seed priming, i.e. soaking seeds overnight in water before sowing, on plant growth and development are consequences of faster germination, emergence, and more vigorous early growth. Results from in-vitro, on-station and on-farm experiments are discussed. Recent work has tested opportunities for resource-poor farmers to use seed priming as a vehicle for applying biofertilisers (Rhizobia). Preliminary results from field experiments suggest that these interventions are very effective over and above the already demonstrated benefits of priming with water alone. In a pot experiment using chickpea, combining a Rhizobium inoculation with seed priming significantly increased nodulation but had little effect on yield. Nevertheless, the results confirmed that Rhizobium inoculation is compatible with on-farm seed priming. Observations in the field have shown that some primed crops show enhanced resistance to disease, either as a consequence of increased vigour, altered phenology, or due to some more fundamental mechanism associated with exposure of seeds to anaerobic conditions during priming. Priming seeds of a highly susceptible cultivar of pearl millet in water for 8 h before sowing significantly reduced the incidence of downy mildew in artificially infected seedlings from 80% to less than 60%.

scholarly journals Effect of Seed Priming on Germination Properties and Seedling Establishment of Cowpea (Vigna sinensis)

2011 ◽  
Vol 3 (4) ◽  
pp. 113-116 ◽  
Author(s):  
Hamdollah ESKANDARI ◽  
Kamyar KAZEMI
Keyword(s):  
Laboratory Tests ◽  
Low Cost ◽  
Germination Rate ◽  
Seedling Emergence ◽  
Laboratory Data ◽  
Seed Priming ◽  
Germination Percentage ◽  
Seed Vigor ◽  
Seedling Vigor ◽  
Contributing Factors

Early emergence and stand establishment of cowpea are considered to be the most important yield-contributing factors in rainfed areas. Laboratory tests and afield experiment were conducted in RCB design in 2011 at a research farm in Ramhormoz, Iran, to evaluate the effects of hydropriming (8, 12 and 16 hours duration) and halo priming (solutions of 1.5% KNO3 and 0.8% NaCl) on seedling vigor and field establishment of cowpea. Analysis of variance of laboratory data showed that hydropriming significantly improved germination rate, seed vigor index, and seedling dry weights. However, germination percentage for seeds primed with KNO3 and non-primed seeds were statistically similar, but higher than those for NaCl priming. Overall, hydropriming treatment was comparatively superior in the laboratory tests. Invigoration of cowpea seeds by hydropriming and NaCl priming resulted in higher seedling emergence and establishment in the field, compared to control and seed priming with KNO3. Seedling emergence rate was also enhanced by priming seeds with water, suggesting that hydropriming is a simple, low cost and environmentally friendly technique for improving seed and seedling vigor of cowpea.

scholarly journals Seed Priming: A Potential Supplement in Integrated Resource Management Under Fragile Intensive Ecosystems

2021 ◽  
Vol 5 ◽  
Author(s):  
O. Siva Devika ◽  
Sonam Singh ◽  
Deepranjan Sarkar ◽  
Prabhakar Barnwal ◽  
Jarupula Suman ◽  
...  
Keyword(s):  
Production Systems ◽  
Low Cost ◽  
Germination Rate ◽  
Cost Benefit ◽  
Seed Priming ◽  
Crop Productivity ◽  
Detailed Examination ◽  
Productivity Cost ◽  
Sustainable Food ◽  
Set Up

A majority of agricultural activities are conducted under fragile lands or set-up. The growth and development of crops are negatively affected due to several biotic and abiotic stresses. In the current situation, research efforts have been diverted toward the short-term approaches that can improve crop performance under changing environments. Seed treatment or priming technology is in a transition phase of its popularity among resource-poor farmers. Suitable policy intervention can boost low-cost techniques to implement them on a larger scale in developing countries and to harness the maximum benefits of sustainable food production systems. Primed seeds have high vigor and germination rate that help in seedling growth and successful crop stand establishment under stress conditions. This review is attempted to assess different seed priming techniques in terms of resource use efficiency, crop productivity, cost–benefit balance, and environmental impacts. Moreover, a comprehensive study of the mechanisms (physiological and biochemical) of seed priming is also elaborated. A detailed examination of the applications of priming technology under diverse agroecosystems can improve our understanding of the adaptive management of natural resources.

IMPROVING THE YIELD OF MUNGBEAN (VIGNA RADIATA) IN THE NORTH WEST FRONTIER PROVINCE OF PAKISTAN USING ON-FARM SEED PRIMING

2004 ◽  
Vol 40 (2) ◽  
pp. 233-244 ◽  
Author(s):  
A. RASHID ◽  
D. HARRIS ◽  
P. A. HOLLINGTON ◽  
M. RAFIQ
Keyword(s):  
Vigna Radiata ◽  
Low Cost ◽  
Seed Priming ◽  
North West ◽  
Yield Increase ◽  
The North ◽  
North West Frontier Province ◽  
Rate Of Decline ◽  
Surface Drying ◽  
On Farm

The effect of ‘on-farm’ seed priming – soaking seeds in water before surface-drying and sowing them – was tested for mungbean (Vigna radiata) in 15 irrigated on-station trials and four sets of rainfed, paired-plot, farmer-participatory trials over four contrasting years from 1999 to 2002 in the North West Frontier Province of Pakistan. The optimum soaking time was found to be between six and eight hours; eight hours was used in all the trials. Of the 19 trials, priming was significantly better than non-priming in 14 with a mean yield increase of 56%. In the remaining five trials there was no difference between treatments but in no case was priming worse than not priming. In a subset of 11 on-station trials in which management was considered to be optimal, yield declined in a linear fashion as the date of sowing was delayed. The rate of decline of about 30 kg ha−1 d−1 after 1st June was similar for both non-primed and primed crops, although the latter declined from a higher base. Farmers' yields were proportional to rainfall over the four years and the mean increase in grain yield due to priming in the 39 trials was 30%. Benefits from priming were the result of a combination of faster germination and emergence and more vigorous growth and development, leading to better crop stands and bigger, more productive plants. It was concluded that ‘on-farm’ seed priming is a low-cost, low-risk technology that has the potential to raise mungbean yields substantially thus making it a more attractive crop for farmers.

Nutripriming with zinc sulphate and borax for early growth and seedling vigour in grain cowpea [Vigna unguiculata (L.) Walp]

2019 ◽  
Author(s):  
Anju B Raj ◽  
Sheeja K Raj ◽  
K. Prathapan ◽  
N.V. Radhakrishnan
Keyword(s):  
Germination Rate ◽  
Seed Priming ◽  
Early Growth ◽  
Research Station ◽  
Seedling Vigour ◽  
Early Seedling Growth ◽  
Vigour Index ◽  
Early Seedling ◽  
Mean Germination Time ◽  
Germination Parameters

The experiment was conducted at Coconut Research Station, Balaramapuram with an objective to study the effect of nutripriming with ZnSO4 and borax on the early growth and seedling vigour and Zn and B content in grain cowpea. Nutripriming treatments had significant effect on germination parameters. Nutripriming with ZnSO4 either at 0.025 or 0.05 per cent recorded higher values for the germination parameters. These treatments recorded 19.68 and 18.51 per centage higher germination than control. Germination index was 1.4 times higher in seeds primed with ZnSO4 @ 0.025 and 0.05 per cent and also recorded higher values for mean daily germination and speed of germination. The highest co-efficient rate of germination and germination rate index was recorded by seed priming with ZnSO4 at 0.05 per cent and it was closely followed by ZnSO4 @ 0.025 per cent for 4 h. Nutripriming with ZnSO4 either @ 0.025 or 0.05 per cent recorded the lowest mean germination time and time to reach 50 per cent germination and also recorded higher Zn and B content. Vigour index also followed the same trend. Hence it can be concluded that nutripriming with ZnSO4 either at 0.025 or 0.05 per cent can be recommended for early seedling growth, seedling vigour and higher Zn and B content in grain cowpea.

ON-FARM SEED PRIMING IN SEMI-ARID AGRICULTURE: DEVELOPMENT AND EVALUATION IN MAIZE, RICE AND CHICKPEA IN INDIA USING PARTICIPATORY METHODS

1999 ◽  
Vol 35 (1) ◽  
pp. 15-29 ◽  
Author(s):  
D. Harris ◽  
A. Joshi ◽  
P. A. Khan ◽  
P. Gothkar ◽  
P. S. Sodhi
Keyword(s):  
Crop Production ◽  
Upland Rice ◽  
Monsoon Season ◽  
Low Cost ◽  
Seed Priming ◽  
Crop Failure ◽  
Rainfed Farming ◽  
Safe Limits ◽  
On Farm

Poor crop establishment was identified as a major constraint on rainfed crop production by farmers in the tribal villages of Rajasthan, Gujarat and Madhya Pradesh served by the Krishak Bharati Cooperative (KRIBHCO) Indo-British Rainfed Farming Project (KRIBP). On-farm seed priming with water was chosen as a low cost, low risk intervention appropriate to the farmers' needs. In vitro screening of the effects of priming on the germination of seeds of local and improved varieties of maize, upland rice and chickpea provided ‘safe limits’ – the maximum length of time for which farmers should prime seeds and which, if exceeded, could lead to seed or seedling damage. Recommended safe limits were 24 h for maize and rice and 10 h for chickpea, with only minor varietal differences. These recommendations were then tested in on-station trials in Dahod, Gujarat. Farmer-managed trials were conducted for chickpea in three villages in the rabi (post-monsoon) season in 1995–96; for maize and upland rice in eight villages in the kharif (monsoon) season in 1996; and for maize and chickpea in 15 villages in the 1996--97 rabi season. Farmers modified these recommendations to ‘overnight’ for all three crops. Evaluation of the technology by farmers involved focus group discussions, matrix ranking exercises and two workshops.Direct benefits in all three crops included faster emergence, better stands and a lower incidence of re-sowing, more vigorous plants, better drought tolerance, earlier flowering, earlier harvest and higher grain yield. Indirect benefits reported were earlier sowing of rabi crops because of the shorter duration of the preceding kharif crop, earlier harvesting of rabi crops that allowed earlier migration from the area, with better chance of obtaining off-season work, and increased willingness to use fertilizers because of reduced risk of crop failure. In matrix ranking exercises in four villages in the kharif 1996, 95% of farmers indicated that, even after only one exposure to the technology, they would prime seed in the following season. Similar exercises in four villages in rabi 1996–97 revealed that 100% of collaborating farmers intended to continue seed priming. From 21 villages, 246 farmers attended two workshops to share their experiences of seed priming and resolved to continue with the technology.

Microextraction and Chromatographic Analysis of Budesonide Epimers in Exhaled Breath Condensate

2020 ◽  
Vol 16 (8) ◽  
pp. 1032-1040
Author(s):  
Laleh Samini ◽  
Maryam Khoubnasabjafari ◽  
Mohamad M. Alimorad ◽  
Vahid Jouyban-Gharamaleki ◽  
Hak-Kim Chan ◽  
...  
Keyword(s):  
Biological Fluids ◽  
Exhaled Breath Condensate ◽  
Low Cost ◽  
Exhaled Breath ◽  
Chromatography Method ◽  
Extraction Solvent ◽  
Drug Concentrations ◽  
Dispersive Liquid Liquid Microextraction ◽  
Breath Condensate

Background: Analysis of drug concentrations in biological fluids is required in clinical sciences for various purposes. Among other biological samples, exhaled breath condensate (EBC) is a potential sample for follow up of drug concentrations. Methods: A dispersive liquid-liquid microextraction (DLLME) procedure followed by a validated liquid chromatography method was employed for the determination of budesonide (BDS) in EBC samples collected using a homemade setup. EBC is a non-invasive biological sample with possible applications for monitoring drug concentrations. The proposed analytical method is validated according to the FDA guidelines using EBC-spiked samples. Its applicability is tested on EBC samples collected from healthy volunteers receiving a single puff of BDS. Results: The best DLLME conditions involved the use of methanol (1 mL) as a disperser solvent, chloroform (200 μL) as an extraction solvent, and centrifugation rate of 3500 rpm for 5 minutes. The method was validated over a concentration range of 21-210 μg·L-1 in EBC. Inter- and intra-day precisions were less than 10% where the acceptable levels are less than 20%. The validated method was successfully applied for the determination of BDS in EBC samples. Conclusion: The findings of this study indicate that the developed method can be used for the extraction and quantification of BDS in EBC samples using a low cost method.

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