scholarly journals Regulation of Vascular Smooth Muscle Cell Stiffness and Adhesion by [Ca2+]i: An Atomic Force Microscopy-Based Study – CORRIGENDUM

2019 ◽  
Vol 25 (1) ◽  
pp. 267-267
Author(s):  
Yi Zhu ◽  
Li He ◽  
Jing Qu ◽  
Yong Zhou
Keyword(s):  
Atomic Force Microscopy ◽  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cell ◽  
Muscle Cell ◽  
Vascular Smooth Muscle Cell ◽  
Cell Stiffness ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Atomic force microscopy reveals the role of vascular smooth muscle cell elasticity in hypertension

2020 ◽  
Author(s):  
Yi Zhu
Keyword(s):  
Atomic Force Microscopy ◽  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cell ◽  
Real Time ◽  
Muscle Cell ◽  
Vascular Smooth Muscle Cell ◽  
Research Subjects ◽  
Force Microscopy ◽  
Atomic Force

AbstractThe vascular smooth muscle cell (VSMC) mechanical properties not only provide intrinsic cellular functions, but also influence many vascular and circulation functions in physiology. In this report, the VSMCs of thoracic aorta from 16 week age Wistar-Kyoto normotensive rats (WKY) and spontaneously hypertensive rats (SHR) were used as research subjects to reveal hypertension mechanism at a single cell level using atomic force microscopy (AFM). The apparent elastic modulus was significantly increased in VSMCs from SHRs compared to those from WKYs. Treatment with cytochalasin D (CD), ML7, Y27632 and lysophosphatidic acid (LPA) modulated VSMC stiffness of WKYs and SHRs. A spectral analysis approach was applied to further investigate the time-dependent change in VSMC elasticity of WKYs and SHRs. This report demonstrated the efficacy of real-time analysis of VSMC elasticity by AFM nano-indentation, and revealed real-time functional differences in biomechanical characteristics of VSMCs with drug treatments.

scholarly journals Atomic Force Microscopy Reveals the Role of Vascular Smooth Muscle Cell Elasticity in Hypertension

2021 ◽  
Author(s):  
Yi Zhu
Keyword(s):  
Atomic Force Microscopy ◽  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cell ◽  
Real Time ◽  
Muscle Cell ◽  
Vascular Smooth Muscle Cell ◽  
Force Microscopy ◽  
Atomic Force ◽  
Wistar Kyoto

The vascular smooth muscle cell (VSMC) mechanical properties not only provide intrinsic cellular functions, but also influence many vascular and circulation functions in physiology. In this report, the VSMCs of thoracic aorta from 16-18 week age Wistar-Kyoto normotensive rats (WKY) and spontaneously hypertensive rats (SHR) were used as research subjects to reveal hypertension mechanism at a single cell level using atomic force microscopy (AFM). The apparent elastic modulus was significantly increased in VSMCs from SHRs compared to those from WKYs. Treatment with cytochalasin D (CD), ML7, Y27632 and lysophosphatidic acid (LPA) modulated VSMC stiffness of WKYs and SHRs. A spectral analysis approach was applied to further investigate the time- dependent change in VSMC elasticity of WKYs and SHRs. This report demonstrated the efficacy of real-time analysis of VSMC elasticity by AFM nano-indentation, and revealed real-time functional differences in biomechanical characteristics of VSMCs with drug treatments.

scholarly journals Augmented Vascular Smooth Muscle Cell Stiffness and Adhesion When Hypertension Is Superimposed on Aging

Hypertension ◽  
2015 ◽  
Vol 65 (2) ◽  
pp. 370-377 ◽  
Author(s):  
Nancy L. Sehgel ◽  
Zhe Sun ◽  
Zhongkui Hong ◽  
William C. Hunter ◽  
Michael A. Hill ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cell ◽  
Muscle Cell ◽  
Vascular Smooth Muscle Cell ◽  
Cell Stiffness

scholarly journals Vascular Smooth Muscle Cell Stiffness and Adhesion to Collagen I Modified by Vasoactive Agonists

PLoS ONE ◽  
2015 ◽  
Vol 10 (3) ◽  
pp. e0119533 ◽  
Author(s):  
Zhongkui Hong ◽  
Kimberley J. Reeves ◽  
Zhe Sun ◽  
Zhaohui Li ◽  
Nicola J. Brown ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cell ◽  
Muscle Cell ◽  
Vascular Smooth Muscle Cell ◽  
Collagen I ◽  
Cell Stiffness ◽  
Vasoactive Agonists

scholarly journals Short Communication: Vascular Smooth Muscle Cell Stiffness As a Mechanism for Increased Aortic Stiffness With Aging

2010 ◽  
Vol 107 (5) ◽  
pp. 615-619 ◽  
Author(s):  
Hongyu Qiu ◽  
Yi Zhu ◽  
Zhe Sun ◽  
Jerome P. Trzeciakowski ◽  
Meredith Gansner ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cell ◽  
Muscle Cell ◽  
Vascular Smooth Muscle Cell ◽  
Aortic Stiffness ◽  
Short Communication ◽  
Cell Stiffness

scholarly journals Regulation of Vascular Smooth Muscle Cell Stiffness and Adhesion by [Ca2+]i: An Atomic Force Microscopy-Based Study

2018 ◽  
Vol 24 (6) ◽  
pp. 708-712 ◽  
Author(s):  
Yi Zhu ◽  
Li He ◽  
Jing Qu ◽  
Yong Zhou
Keyword(s):  
Atomic Force Microscopy ◽  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Actin ◽  
Cell Stiffness ◽  
Adhesive Properties ◽  
Acetoxymethyl Ester ◽  
Altered Expression ◽  
Force Microscopy ◽  
Atomic Force

AbstractThe intracellular concentration of calcium ion ([Ca2+]i) is a critical regulator of cell signaling and contractility of vascular smooth muscle cells (VSMCs). In this study, we employed an atomic force microscopy (AFM) nanoindentation-based approach to investigate the role of [Ca2+]i in regulating the cortical elasticity of rat cremaster VSMCs and the ability of rat VSMCs to adhere to fibronectin (Fn) matrix. Elevation of [Ca2+]i by ionomycin treatment increased rat VSMC stiffness and cell adhesion to Fn-biofunctionalized AFM probes, whereas attenuation of [Ca2+]i by 1,2-Bis (2-aminophenoxy)ethane-N,N,N’,N’-tetraacetic acid tetrakis (acetoxymethyl ester) (BAPTA-AM) treatment decreased the mechanical and matrix adhesive properties of VSMCs. Furthermore, we found that ionomycin/BAPTA-AM treatments altered expression of α5 integrin subunits and α smooth muscle actin in rat VSMCs. These data suggest that [Ca2+]i regulates VSMC elasticity and adhesion to the extracellular matrix by a potential mechanism involving changing dynamics of the integrin–actin cytoskeleton axis.

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