The use of the in vitro gas production technique to investigate the effect of substrate on the partitioning between microbial biomass production and the yield of fermentation products

1999 ◽  
Vol 1999 ◽  
pp. 36-36 ◽  
Author(s):  
C. Rymer ◽  
D.I. Givens
Keyword(s):  
Microbial Biomass ◽  
Biomass Yield ◽  
Negative Relationship ◽  
Poor Quality ◽  
Gas Production ◽  
The Other ◽  
Production Technique ◽  
Fermentation Products ◽  
In Vitro Gas Production

The in vitro gas production technique has been developed as a means of predicting the degradability of feeds. However, it was pointed out by Blümmel et al. (1997) that attention should be given to the other products of the incubation as well. Degraded feeds may be incorporated directly into microbial biomass, or they may be fermented to produce VFA and gas. One of the objectives of this experiment was to determine whether the proportion of degraded substrate that was partitioned to gas production varied with different feeds. Blümmel et al. (1997) observed a negative relationship between gas yield (ml gas produced/g substrate degraded) and microbial biomass yield (mg biomass/g substrate degraded) when poor quality forages were incubated. A second objective of this work was to determine whether this relationship was also observed when higher quality feeds were used.

scholarly journals The nutritional evaluation of forage-based mixed rations in New Zealand using an in vitro gas production technique. 1: analytical survey

2021 ◽  
pp. 1-6
Author(s):  
N.D. Meads ◽  
R. Tahmasbi ◽  
N. Jantasila
Keyword(s):  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Environmental Science ◽  
Ghg Emissions ◽  
Negative Relationship ◽  
Real Data ◽  
Gas Production ◽  
Production Technique ◽  
In Vitro Gas Production

Greenhouse gas (GHG) emissions from livestock are an important consideration in environmental science. Estimating GHG production can be problematic at a farm or animal level, and requires controlled conditions to produce real data. An in vitro gas production technique (IVGPT) was developed to evaluate forage-based total mixed rations in digestion kinetics and GHG production. Two hundred and sixty samples of complete mixed rations (MR), which included a pasture component used in commercial lactating dairy herds, were collected around NZ across three calendar years, 2017-2019. Twenty of the 260 samples were 100% total mixed rations (TMR) with no pasture content. The samples were submitted for proximate analysis as well as IVGPT to generate GHG production figures. The results showed an average total gas production (TGP) of 129.82 ml/g dry matter (DM), 78.6% true digestibility (TDMD), 125.06 mg/g DM microbial biomass (MB), 20.16 g CH4/kg DM, and 12.8 MJME/kg DM. The average nutrient composition was dry matter (DM) 31.55%, crude protein (CP) 21.85%, neutral detergent fibre (NDF) 44.35%, and starch 7.03%. The IVGPT CH4 production was negatively correlated to NDF (r=-0.312), ADF (r=-0.193), TGP (r=-0.216), and was positively correlated with TDMD (r=0.250), apparent digestibility (ADMD) (r=0.614), starch (r=0.117) and volatile fatty acids (r=0.538). The MR diet showed a strong positive relationship with ADMD digestibility (P=0.01) and a negative relationship with fibre content (NDF, P=0.01 and ADF, P=0.01). However, CH4 production reduced linearly with increasing TGP (P=0.01). The results indicated that a greater CH4 production may be related to higher digestibility of mixed ration.

Effect of the ratio of maize cob and husk to napier Pakchong 1 silage on nutritive value and in vitro gas production of rumen fluid of Thai native cattle

2017 ◽  
Vol 57 (8) ◽  
pp. 1603 ◽  
Author(s):  
S. Yammuen-art ◽  
P. Somrak ◽  
C. Phatsara
Keyword(s):  
Microbial Biomass ◽  
Nutritive Value ◽  
Biomass Yield ◽  
Rumen Fluid ◽  
Gas Production ◽  
Average Weight ◽  
In Vitro Gas Production ◽  
Native Cattle ◽  
Maize Cob

The present study evaluated the chemical composition and in vitro ruminal digestibility of napier Pakchong 1 silage combined with maize cob and husk in different ratios. The napier Pakchong 1 grass was harvested at 45 days of maturity. The napier Pakchong 1 grass was ensiled with maize cob and husk at ratios of 1:5, 1:10 and 1:15. Three rumen fistulated Thai native cattle (White Lamphun cattle) with an average weight of 154 ± 4.7 kg were used to determine ruminal digestibility by in vitro gas-production technique. Gas production was recorded after incubating for 2, 4, 8, 12, 24, 48, 72 and 96 h. The microbial biomass yield was determined after incubating for 24 h. DM, NDF and ADF of maize cob and husk mixed with napier Pakchong 1 silage declined by increasing the proportion of napier Pakchong 1 grass, while gas production after 4–10 h of incubating maize cob and husk mixed with napier Pakchong 1 grass increased by increasing the proportion of napier Pakchong 1 grass. The metabolisable energy, organic matter digestibility and microbial biomass yield did not differ among the different ratios. The results of the study suggested a recommended ratio of maize cob and husk to napier Pakchong 1 grass of 1:10. The ensiling fermentation increased the proportion of protein in the roughage, which lead to increased in vitro gas production of roughage.

scholarly journals Corrigendum to: Effect of the ratio of maize cob and husk to napier Pakchong 1 silage on nutritive value and in vitro gas production of rumen fluid of Thai native cattle

2019 ◽  
Vol 59 (3) ◽  
pp. 600
Author(s):  
S. Yammuen-art ◽  
P. Somrak ◽  
C. Phatsara
Keyword(s):  
Microbial Biomass ◽  
Nutritive Value ◽  
Biomass Yield ◽  
Rumen Fluid ◽  
Gas Production ◽  
Average Weight ◽  
In Vitro Gas Production ◽  
Native Cattle ◽  
Maize Cob

The present study evaluated the chemical composition and in vitro ruminal digestibility of napier Pakchong 1 silage combined with maize cob and husk in different ratios. The napier Pakchong 1 grass was harvested at 45 days of maturity. The napier Pakchong 1 grass was ensiled with maize cob and husk at ratios of 1:5, 1:10 and 1:15. Three rumen fistulated Thai native cattle (White Lamphun cattle) with an average weight of 154 ± 4.7 kg were used to determine ruminal digestibility by in vitro gas-production technique. Gas production was recorded after incubating for 2, 4, 8, 12, 24, 48, 72 and 96 h. The microbial biomass yield was determined after incubating for 24 h. DM, NDF and ADF of maize cob and husk mixed with napier Pakchong 1 silage declined by increasing the proportion of napier Pakchong 1 grass, while gas production after 4–10 h of incubating maize cob and husk mixed with napier Pakchong 1 grass increased by increasing the proportion of napier Pakchong 1 grass. The metabolisable energy, organic matter digestibility and microbial biomass yield did not differ among the different ratios. The results of the study suggested a recommended ratio of maize cob and husk to napier Pakchong 1 grass of 1:10. The ensiling fermentation increased the proportion of protein in the roughage, which lead to increased in vitro gas production of roughage.

Estimation of fermentable energy by the automatic in vitro gas production technique for a selection of feedstuffs incubated alone and in combination

1998 ◽  
Vol 1998 ◽  
pp. 69-69
Author(s):  
S. Fakhri ◽  
A. R. Moss ◽  
D.I. Givens ◽  
E. Owen
Keyword(s):  
Organic Matter ◽  
Incubation Period ◽  
Gas Production ◽  
Production Technique ◽  
In Vitro Gas Production ◽  
Fe Content ◽  
The Individual ◽  
Gas Volume ◽  
Selection Of

The gas production (GP) technique has previously been used to estimate the gas volume (fermentable energy (FE)) of compound feed ingredients for ruminants (Newbold et al., 1996). It was shown that the FE content of feed mixtures was represented by the combination of the total gas from the incubation of the individual feeds. However this additivity might not be consistent throughout the incubation period. The objectives were to test whether 1. other GP parameters give better estimates of FE for simple mixtures and are they additive; 2. whether organic matter apparently degraded in the rumen (OMADR) explain differences in GP; and 3. to find out if there are any other better measures than OMADR for estimating FE.

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