Applications of In-Source Fragmentation of Protein Ions for Direct Sequence Analysis by Delayed Extraction MALDI-TOF Mass Spectrometry

1998 ◽  
Vol 70 (20) ◽  
pp. 4410-4416 ◽  
Author(s):  
Viswanatham Katta ◽  
David T. Chow ◽  
Michael F. Rohde
Keyword(s):  
Mass Spectrometry ◽  
Sequence Analysis ◽  
Direct Sequence ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof ◽  
Protein Ions ◽  
Delayed Extraction ◽  
Direct Sequence Analysis

scholarly journals The use of ITS DNA sequence analysis and MALDI-TOF mass spectrometry in diagnosing an infection withFusarium proliferatum

2008 ◽  
Vol 17 (11) ◽  
pp. 965-971 ◽  
Author(s):  
Florian Seyfarth ◽  
Mirjana Ziemer ◽  
Herbert G. Sayer ◽  
Anke Burmester ◽  
Marcel Erhard ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Sequence Analysis ◽  
Dna Sequence ◽  
Dna Sequence Analysis ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof

scholarly journals Detecting the Site of Phosphorylation in Phosphopeptides without Loss of Phosphate Group Using MALDI TOF Mass Spectrometry

2008 ◽  
Vol 3 ◽  
pp. ACI.S497 ◽  
Author(s):  
Medicharla V. Jagannadham ◽  
Ramakrishnan Nagaraj
Keyword(s):  
Mass Spectrometry ◽  
Negative Ions ◽  
Maldi Mass Spectrometry ◽  
Phosphate Group ◽  
Maldi Tof Mass Spectrometry ◽  
Linear Mode ◽  
Maldi Tof ◽  
Phosphorylated Peptides ◽  
Delayed Extraction ◽  
Phosphate Groups

Phosphopeptides with one and four phosphate groups were characterized by MALDI mass spectrometry. The molecular ion of monophosphopeptide could be detected both as positive and negative ions by MALDI TOF with delayed extraction (DE) and in the reflector mode. The tetraphospho peptide could be detected in linear mode. When MS/MS spectra of the monophospho peptides were obtained in a MALDI TOF TOF instrument by CID, b and y ions with the intact phosphate group were observed, in addition the b and y ions without the phosphate group. Our study indicates that it is possible to detect phosphorylated peptides with out the loss of phosphate group by MALDI TOF as well as MALDI TOF TOF instruments with delayed extraction and in the reflector mode.

Delineation of Stenotrophomonas spp. by multi-locus sequence analysis and MALDI-TOF mass spectrometry

2011 ◽  
Vol 34 (1) ◽  
pp. 35-39 ◽  
Author(s):  
Zinaida Vasileuskaya-Schulz ◽  
Sabine Kaiser ◽  
Thomas Maier ◽  
Markus Kostrzewa ◽  
Daniel Jonas
Keyword(s):  
Mass Spectrometry ◽  
Sequence Analysis ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof
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