Self-Powered Biosensing Platform Based on “Signal-On” Enzymatic Biofuel Cell for DNA Methyltransferase Activity Analysis and Inhibitor Screening

2020 ◽  
Vol 92 (7) ◽  
pp. 5426-5430 ◽  
Author(s):  
Chengcheng Gu ◽  
Panpan Gai ◽  
Xinke Kong ◽  
Ting Hou ◽  
Feng Li
Keyword(s):  
Dna Methyltransferase ◽  
Activity Analysis ◽  
Biofuel Cell ◽  
Methyltransferase Activity ◽  
Inhibitor Screening ◽  
Enzymatic Biofuel Cell ◽  
Self Powered

Fluorescent copper nanoclusters as a nano-dye for DNA methyltransferase activity analysis and inhibitor screening

2018 ◽  
Vol 559 ◽  
pp. 5-10 ◽  
Author(s):  
Dengpeng Gao ◽  
Hongyue Zhang ◽  
Yafei Xu ◽  
Yun Liu ◽  
Huiying Xu ◽  
...  
Keyword(s):  
Dna Methyltransferase ◽  
Activity Analysis ◽  
Methyltransferase Activity ◽  
Copper Nanoclusters ◽  
Inhibitor Screening

scholarly journals A Self-Powered Biosensor for the Detection of Glutathione

Biosensors ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 114 ◽  
Author(s):  
Brandon G. Roy ◽  
Julia L. Rutherford ◽  
Anna E. Weaver ◽  
Kevin Beaver ◽  
Michelle Rasmussen
Keyword(s):  
Detection Limit ◽  
Glucose Oxidase ◽  
Power Output ◽  
Low Cost ◽  
Linear Range ◽  
Biofuel Cell ◽  
Bilirubin Oxidase ◽  
Biological Molecule ◽  
Enzymatic Biofuel Cell ◽  
Self Powered

Glutathione is an important biological molecule which can be an indicator of numerous diseases. A method for self-powered detection of glutathione levels in solution has been developed using an enzymatic biofuel cell. The device consists of a glucose oxidase anode and a bilirubin oxidase cathode. For the detection of glutathione, the inhibition of bilirubin oxidase leads to a measurable decrease in current and power output. The reported method has a detection limit of 0.043 mM and a linear range up to 1.7 mM. Being able to detect a range of concentrations can be useful in evaluating a patient’s health. This method has the potential to be implemented as a quick, low-cost alternative to previously reported methods.

Highly sensitive methyltransferase activity assay and inhibitor screening based on fluorescence quenching of graphene oxide integrated with the site-specific cleavage of restriction endonuclease

2014 ◽  
Vol 50 (73) ◽  
pp. 10691-10694 ◽  
Author(s):  
Lijuan Ji ◽  
Zhewei Cai ◽  
Yingdan Qian ◽  
Ping Wu ◽  
Hui Zhang ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Fluorescence Quenching ◽  
Restriction Endonuclease ◽  
Dna Methyltransferase ◽  
Activity Assay ◽  
Methyltransferase Activity ◽  
Site Specific ◽  
Inhibitor Screening ◽  
Selective Approach ◽  
Highly Sensitive

A sensitive and selective approach for the DNA methyltransferase activity assay and MTase inhibitor screening is reported.

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