scholarly journals Infrared Matrix-Assisted Laser Desorption Electrospray Ionization Mass Spectrometry Imaging of Human Hair to Characterize Longitudinal Profiles of the Antiretroviral Maraviroc for Adherence Monitoring

2019 ◽  
Vol 91 (16) ◽  
pp. 10816-10822 ◽  
Author(s):  
William M. Gilliland ◽  
Heather M.A. Prince ◽  
Amanda Poliseno ◽  
Angela D.M. Kashuba ◽  
Elias P. Rosen
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
Mass Spectrometry Imaging ◽  
Desorption Electrospray Ionization ◽  
Laser Desorption ◽  
Ionization Mass ◽  
Longitudinal Profiles ◽  
Adherence Monitoring ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

scholarly journals Enhancing Metabolomic Coverage in Positive Ionization Mode Using Dicationic Reagents by Infrared Matrix-Assisted Laser Desorption Electrospray Ionization

Metabolites ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 810
Author(s):  
Ying Xi ◽  
David C. Muddiman
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
Rat Liver ◽  
Mass Spectrometry Imaging ◽  
Desorption Electrospray Ionization ◽  
Laser Desorption ◽  
Ionization Mass ◽  
Ionization Mode ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

Mass spectrometry imaging is a powerful tool to analyze a large number of metabolites with their spatial coordinates collected throughout the sample. However, the significant differences in ionization efficiency pose a big challenge to metabolomic mass spectrometry imaging. To solve the challenge and obtain a complete data profile, researchers typically perform experiments in both positive and negative ionization modes, which is time-consuming. In this work, we evaluated the use of the dicationic reagent, 1,5-pentanediyl-bis(1-butylpyrrolidinium) difluoride (abbreviated to [C5(bpyr)2]F2) to detect a broad range of metabolites in the positive ionization mode by infrared matrix-assisted laser desorption electrospray ionization mass spectrometry imaging (IR-MALDESI MSI). [C5(bpyr)2]F2 at 10 µM was doped in 50% MeOH/H2O (v/v) electrospray solvent to form +1 charged adducted ions with anionic species (−1 charged) through post-electrospray ionization. This method was demonstrated with sectioned rat liver and hen ovary. A total of 73 deprotonated metabolites from rat liver tissue sections were successfully adducted with [C5(bpyr)2]2+ and putatively identified in the adducted positive ionization polarity, along with 164 positively charged metabolite ions commonly seen in positive ionization mode, which resulted in 44% increased molecular coverage. In addition, we were able to generate images of hen ovary sections showing their morphological features. Following-up tandem mass spectrometry (MS/MS) indicated that this dicationic reagent [C5(bpyr)2]2+ could form ionic bonds with the headgroup of glycerophospholipid ions. The addition of the dicationic reagent [C5(bpyr)2]2+ in the electrospray solvent provides a rapid and effective way to enhance the detection of metabolites in positive ionization mode.

scholarly journals Infrared matrix-assisted laser desorption electrospray ionization mass spectrometry imaging analysis of biospecimens

The Analyst ◽  
2016 ◽  
Vol 141 (18) ◽  
pp. 5236-5245 ◽  
Author(s):  
M. T. Bokhart ◽  
D. C. Muddiman
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
Mass Spectrometry Imaging ◽  
Desorption Electrospray Ionization ◽  
Laser Desorption ◽  
Ionization Mass ◽  
Imaging Analysis ◽  
Depth Analysis ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

Infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) mass spectrometry imaging (MSI) is a versatile imaging technique capable of in-depth analysis for a wide variety of biospecimens.

scholarly journals Mass spectrometry imaging (MSI) of fresh bones using infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI)

2019 ◽  
Vol 11 (46) ◽  
pp. 5929-5938 ◽  
Author(s):  
Sitora Khodjaniyazova ◽  
Nicholas J. Hanne ◽  
Jacqueline H. Cole ◽  
David C. Muddiman
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
Mass Spectrometry Imaging ◽  
Desorption Electrospray Ionization ◽  
Laser Desorption ◽  
Direct Analysis ◽  
Chemical Treatments ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

Direct analysis and IR-MALDESI mass spectrometry imaging of fresh mouse bones that underwent no chemical treatments other than flash-freezing.

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