Moldable Perfluoropolyether–Polyethylene Glycol Networks with Tunable Wettability and Solvent Resistance for Rapid Prototyping of Droplet Microfluidics

2018 ◽  
Vol 30 (8) ◽  
pp. 2583-2588 ◽  
Author(s):  
Heon-Ho Jeong ◽  
Syung Hun Han ◽  
Sagar Yadavali ◽  
Junhyong Kim ◽  
David Issadore ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Rapid Prototyping ◽  
Droplet Microfluidics ◽  
Solvent Resistance

scholarly journals Acoustic focusing of beads and cells in hydrogel droplets

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Anna Fornell ◽  
Hannah Pohlit ◽  
Qian Shi ◽  
Maria Tenje
Keyword(s):  
Polyethylene Glycol ◽  
Uv Light ◽  
Precursor Solution ◽  
Microfluidic System ◽  
Nodal Line ◽  
Droplet Microfluidics ◽  
Acoustic Standing Wave ◽  
Centre Line ◽  
Half Wavelength ◽  
Acoustic Focusing

AbstractThe generation of hydrogel droplets using droplet microfluidics has emerged as a powerful tool with many applications in biology and medicine. Here, a microfluidic system to control the position of particles (beads or astrocyte cells) in hydrogel droplets using bulk acoustic standing waves is presented. The chip consisted of a droplet generator and a 380 µm wide acoustic focusing channel. Droplets comprising hydrogel precursor solution (polyethylene glycol tetraacrylate or a combination of polyethylene glycol tetraacrylate and gelatine methacrylate), photoinitiator and particles were generated. The droplets passed along the acoustic focusing channel where a half wavelength acoustic standing wave field was generated, and the particles were focused to the centre line of the droplets (i.e. the pressure nodal line) by the acoustic force. The droplets were cross-linked by exposure to UV-light, freezing the particles in their positions. With the acoustics applied, 89 ± 19% of the particles (polystyrene beads, 10 µm diameter) were positioned in an area ± 10% from the centre line. As proof-of-principle for biological particles, astrocytes were focused in hydrogel droplets using the same principle. The viability of the astrocytes after 7 days in culture was 72 ± 22% when exposed to the acoustic focusing compared with 70 ± 19% for samples not exposed to the acoustic focusing. This technology provides a platform to control the spatial position of bioparticles in hydrogel droplets, and opens up for the generation of more complex biological hydrogel structures.

scholarly journals Rapid prototyping of fluoropolymer microchannels by xurography for improved solvent resistance

2018 ◽  
Vol 12 (6) ◽  
pp. 064105 ◽  
Author(s):  
Takuma Hizawa ◽  
Atsushi Takano ◽  
Pravien Parthiban ◽  
Patrick S. Doyle ◽  
Eiji Iwase ◽  
...  
Keyword(s):  
Rapid Prototyping ◽  
Solvent Resistance

Immunocytochemical detection of hepatic carbohydrate metabolic enzymes: Improved resolution with polyethylene glycol embedding and visio-bond semithin sections

1992 ◽  
Vol 50 (1) ◽  
pp. 792-793
Author(s):  
Kuixiong Gao ◽  
Randal E. Morris ◽  
Bruce F. Giffin ◽  
Robert R. Cardell
Keyword(s):  
Polyethylene Glycol ◽  
Glycogen Phosphorylase ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Glycogen Synthase ◽  
Metabolic Enzymes ◽  
Silver Stain ◽  
Accurate Localization ◽  
Immunocytochemical Detection ◽  
Semithin Sections ◽  
Parenchymal Cells

Several enzymes are involved in the regulation of anabolic and catabolic pathways of carbohydrate metabolism in liver parenchymal cells. The lobular distribution of glycogen synthase (GS), phosphoenolpyruvate carboxykinase (PEPCK) and glycogen phosphorylase (GP) was studied by immunocytochemistry using cryosections of normal fed and fasted rat liver. Since sections of tissue embedded in polyethylene glycol (PEG) show good morphological preservation and increased detectability for immunocytochemical localization of antigenic sites, and semithin sections of Visio-Bond (VB) embedded tissue provide higher resolution of cellular structure, we applied these techniques and immunogold-silver stain (IGSS) for a more accurate localization of hepatic carbohydrate metabolic enzymes.

Directional observation on lipid of male nectary of Lindera megaphylla hemsl. by the rapid embedding method with polyethylene glycol (PEG)

1990 ◽  
Vol 48 (3) ◽  
pp. 718-719
Author(s):  
Dai Dalin ◽  
Guo Jianmin
Keyword(s):  
Electron Microscope ◽  
Polyethylene Glycol ◽  
Chemical Reaction ◽  
Traditional Method ◽  
Osmium Tetroxide ◽  
Unsaturated Lipid ◽  
Embedding Method ◽  
Long Period ◽  
New Methods

Lipid cytochemistry has not yet advanced far at the EM level. A major problem has been the loss of lipid during dehydration and embedding. Although the adoption of glutaraldehyde and osmium tetroxide accelerate the chemical reaction of lipid and osmium tetroxide can react on the double bouds of unsaturated lipid to from the osmium black, osmium tetroxide can be reduced in saturated lipid and subsequently some of unsaturated lipid are lost during dehydration. In order to reduce the loss of lipid by traditional method, some researchers adopted a few new methods, such as the change of embedding procedure and the adoption of new embedding media, to solve the problem. In a sense, these new methods are effective. They, however, usually require a long period of preparation. In this paper, we do research on the fiora nectary strucure of lauraceae by the rapid-embedding method wwith PEG under electron microscope and attempt to find a better method to solve the problem mentioned above.

Polyethylene glycol-mediated direct gene transfer in Nicotiana spp.

1992 ◽  
Vol 85 (2) ◽  
pp. 345-351 ◽  
Author(s):  
Gokarna B. Gharti-Chhetri ◽  
Wichai Cherdshewasart ◽  
Jocelyne Dewulf ◽  
Michel Jacobs ◽  
loan Negrutiu
Keyword(s):  
Polyethylene Glycol ◽  
Gene Transfer ◽  
Direct Gene Transfer ◽  
Direct Gene

Medical rapid prototyping (MRP) in adult- and paediatric cardiac surgery

2011 ◽  
Vol 59 (S 01) ◽  
Author(s):  
R Sodian ◽  
S Haeberle ◽  
S Weber ◽  
T Lueth ◽  
A Beiras-Fernandez ◽  
...  
Keyword(s):  
Cardiac Surgery ◽  
Rapid Prototyping ◽  
Paediatric Cardiac Surgery
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