Discovery of a Potential Liver Fibrosis Inhibitor from a Mushroom Endophytic Fungus by Genome Mining of a Silent Biosynthetic Gene Cluster

2021 ◽  
Vol 69 (38) ◽  
pp. 11303-11310
Author(s):  
Jin-Tao Cheng ◽  
Han-Min Wang ◽  
Jia-Hui Yu ◽  
Chen-Fan Sun ◽  
Fei Cao ◽  
...  
Keyword(s):  
Liver Fibrosis ◽  
Gene Cluster ◽  
Endophytic Fungus ◽  
Genome Mining ◽  
Biosynthetic Gene Cluster ◽  
Biosynthetic Gene

scholarly journals Bioactivity-Guided Genome Mining Reveals the Lomaiviticin Biosynthetic Gene Cluster inSalinispora tropica

ChemBioChem ◽  
2013 ◽  
Vol 14 (8) ◽  
pp. 955-962 ◽  
Author(s):  
Roland D. Kersten ◽  
Amy L. Lane ◽  
Markus Nett ◽  
Taylor K. S. Richter ◽  
Brendan M. Duggan ◽  
...  
Keyword(s):  
Gene Cluster ◽  
Genome Mining ◽  
Biosynthetic Gene Cluster ◽  
Biosynthetic Gene

scholarly journals Correction: Genome mining and molecular characterization of the biosynthetic gene cluster of a diterpenic meroterpenoid, 15-deoxyoxalicine B, in Penicillium canescens

2016 ◽  
Vol 7 (3) ◽  
pp. 2440-2440 ◽  
Author(s):  
Junko Yaegashi ◽  
Jillian Romsdahl ◽  
Yi-Ming Chiang ◽  
Clay C. C. Wang
Keyword(s):  
Gene Cluster ◽  
Molecular Characterization ◽  
Genome Mining ◽  
Biosynthetic Gene Cluster ◽  
Biosynthetic Gene ◽  
Penicillium Canescens

Correction for ‘Genome mining and molecular characterization of the biosynthetic gene cluster of a diterpenic meroterpenoid, 15-deoxyoxalicine B, in Penicillium canescens’ by Junko Yaegashi et al., Chem. Sci., 2015, 6, 6537–6544.

Genome Mining-Directed Activation of a Silent Angucycline Biosynthetic Gene Cluster inStreptomyces chattanoogensis

ChemBioChem ◽  
2014 ◽  
Vol 16 (3) ◽  
pp. 496-502 ◽  
Author(s):  
Zhenxing Zhou ◽  
Qingqing Xu ◽  
Qingting Bu ◽  
Yuanyang Guo ◽  
Shuiping Liu ◽  
...  
Keyword(s):  
Gene Cluster ◽  
Genome Mining ◽  
Biosynthetic Gene Cluster ◽  
Biosynthetic Gene

Genome Mining of the Hitachimycin Biosynthetic Gene Cluster: Involvement of a Phenylalanine-2,3-aminomutase in Biosynthesis

ChemBioChem ◽  
2015 ◽  
Vol 16 (6) ◽  
pp. 909-914 ◽  
Author(s):  
Fumitaka Kudo ◽  
Koichi Kawamura ◽  
Asuka Uchino ◽  
Akimasa Miyanaga ◽  
Mario Numakura ◽  
...  
Keyword(s):  
Gene Cluster ◽  
Genome Mining ◽  
Biosynthetic Gene Cluster ◽  
Biosynthetic Gene

scholarly journals Identification of the pyranonigrin A biosynthetic gene cluster by genome mining in Penicillium thymicola IBT 5891

AIChE Journal ◽  
2018 ◽  
Vol 64 (12) ◽  
pp. 4182-4186 ◽  
Author(s):  
Man-Cheng Tang ◽  
Yi Zou ◽  
Danielle Yee ◽  
Yi Tang
Keyword(s):  
Gene Cluster ◽  
Genome Mining ◽  
Biosynthetic Gene Cluster ◽  
Biosynthetic Gene

scholarly journals Antimicrobial Activity and Identification of the Biosynthetic Gene Cluster of X-14952B From Streptomyces sp. 135

2021 ◽  
Vol 12 ◽  
Author(s):  
Na Li ◽  
Simin Chen ◽  
Zhiqiang Yan ◽  
Jinhua Han ◽  
Yongquan Ta ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antifungal Activity ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Cluster ◽  
Genome Mining ◽  
Biosynthetic Gene Cluster ◽  
Rrna Gene ◽  
Biosynthetic Gene ◽  
Type Strains

The bacterial genus Streptomyces is an important source of antibiotics, and genome mining is a valuable tool to explore the potential of microbial biosynthesis in members of this genus. This study reports an actinomycete strain 135, which was isolated from Qinghai-Tibet Plateau in China and displayed broad antimicrobial activity. The fermentation broth of strain 135 displayed strong antifungal activity (>70%) against Sclerotinia sclerotiorum, Botrytis cinerea, Valsa mali, Phytophthora capsici, Glomerella cingulata, Magnaporthe grisea, Bipolaris maydis, Exserohilum turcicum in vitro, meanwhile possessed significant preventive and curative efficacy against S. sclerotiorum, Gaeumannomyces graminis, and P. capsici on rape leaves (54.04 and 74.18%), wheat (90.66 and 67.99%), and pepper plants (79.33 and 66.67%). X-14952B showed the greatest antifungal activity against S. sclerotiorum and Fusarium graminearum which the 50% inhibition concentration (EC50) were up to 0.049 and 0.04 μg/mL, respectively. Characterization of strain 135 using a polyphasic approach revealed that the strain displayed typical features of the genus Streptomyces. 16S rRNA gene sequencing and phylogenetic analysis demonstrated that the isolate was most closely related to and formed a clade with Streptomyces huasconensis HST28T (98.96% 16S rRNA gene sequence similarity). Average nucleotide identity (ANI) and DNA-DNA hybridization (DDH) values in strain 135 and related type strains were both below the threshold of species determination (91.39 and 56.5%, respectively). OrthoANI values between strain 135 and related type strains are under the cutoff of determining species (<95%). The biosynthetic gene cluster (BGC) designated to X-14952B biosynthesis was identified through genome mining and the possible biosynthesis process was deduced.

scholarly journals Genome Mining and Metabolomics Uncover a Rare d-Capreomycidine Containing Natural Product and Its Biosynthetic Gene Cluster

2020 ◽  
Vol 15 (11) ◽  
pp. 3013-3020
Author(s):  
James H. Tryon ◽  
Jennifer C. Rote ◽  
Li Chen ◽  
Matthew T. Robey ◽  
Marvin M. Vega ◽  
...  
Keyword(s):  
Natural Product ◽  
Gene Cluster ◽  
Genome Mining ◽  
Biosynthetic Gene Cluster ◽  
Biosynthetic Gene

scholarly journals Genome mining unearths a hybrid nonribosomal peptide synthetase-like-pteridine synthase biosynthetic gene cluster

eLife ◽  
2017 ◽  
Vol 6 ◽  
Author(s):  
Hyun Bong Park ◽  
Corey E Perez ◽  
Karl W Barber ◽  
Jesse Rinehart ◽  
Jason M Crawford
Keyword(s):  
Gene Cluster ◽  
Genetic Locus ◽  
Genome Mining ◽  
Biosynthetic Gene Cluster ◽  
Nonribosomal Peptide Synthetase ◽  
Biosynthetic Gene ◽  
Nonribosomal Peptide ◽  
Redox Active ◽  
Peptide Synthetase ◽  
Phenotypic Variant

Nonribosomal peptides represent a large class of metabolites with pharmaceutical relevance. Pteridines, such as pterins, folates, and flavins, are heterocyclic metabolites that often serve as redox-active cofactors. The biosynthetic machineries for construction of these distinct classes of small molecules operate independently in the cell. Here, we discovered an unprecedented nonribosomal peptide synthetase-like-pteridine synthase hybrid biosynthetic gene cluster in Photorhabdus luminescens using genome synteny analysis. P. luminescens is a Gammaproteobacterium that undergoes phenotypic variation and can have both pathogenic and mutualistic roles. Through extensive gene deletion, pathway-targeted molecular networking, quantitative proteomic analysis, and NMR, we show that the genetic locus affects the regulation of quorum sensing and secondary metabolic enzymes and encodes new pteridine metabolites functionalized with cis-amide acyl-side chains, termed pepteridine A (1) and B (2). The pepteridines are produced in the pathogenic phenotypic variant and represent the first reported metabolites to be synthesized by a hybrid NRPS-pteridine pathway. These studies expand our view of the combinatorial biosynthetic potential available in bacteria.

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