scholarly journals Extracellular Electron Transfer Enables Cellular Control of Cu(I)-Catalyzed Alkyne–Azide Cycloaddition

2022 ◽  
Author(s):  
Gina Partipilo ◽  
Austin J. Graham ◽  
Brian Belardi ◽  
Benjamin K. Keitz
Keyword(s):  
Electron Transfer ◽  
Extracellular Electron Transfer ◽  
Cellular Control

scholarly journals Extracellular Electron Transfer Enables Cellular Control of Cu(I)-catalyzed Alkyne-Azide Cycloaddition

2021 ◽  
Author(s):  
Gina Partipilo ◽  
Austin J. Graham ◽  
Brian Belardi ◽  
Benjamin K. Keitz
Keyword(s):  
Electron Transfer ◽  
Mammalian Cells ◽  
Shewanella Oneidensis ◽  
Anaerobic Respiration ◽  
Central Carbon Metabolism ◽  
Metal Species ◽  
Extracellular Electron Transfer ◽  
Metal Catalyst ◽  
Genetic Circuits ◽  
Cellular Control

AbstractExtracellular electron transfer (EET) is an anaerobic respiration process that couples carbon oxidation to the reduction of metal species. In the presence of a suitable metal catalyst, EET allows for cellular metabolism to control a variety of synthetic transformations. Here, we report the use of EET from the model electroactive bacterium Shewanella oneidensis for metabolic and genetic control over Cu(I)-catalyzed Alkyne-Azide Cycloaddition (CuAAC). CuAAC conversion under anaerobic and aerobic conditions was dependent on live, actively respiring S. oneidensis cells. In addition, reaction progress and kinetics could be further manipulated by tailoring the central carbon metabolism of S. oneidensis. Similarly, CuAAC activity was dependent on specific EET pathways and could be manipulated using inducible genetic circuits controlling the expression of EET-relevant proteins including MtrC, MtrA, and CymA. EET-driven CuAAC also exhibited modularity and robustness in ligand tolerance and substrate scope. Furthermore, the living nature of this system could be exploited to perform multiple reaction cycles without requiring regeneration, something inaccessible to traditional chemical reductants. Finally, S. oneidensis enabled bioorthogonal CuAAC membrane labelling on live mammalian cells without affecting cell viability, suggesting that S. oneidensis can act as a dynamically tunable biocatalyst in complex environments. In summary, our results demonstrate how EET can expand the reaction scope available to living systems by enabling cellular control of CuAAC.

scholarly journals Modeling biofilms with dual extracellular electron transfer mechanisms

2013 ◽  
Vol 15 (44) ◽  
pp. 19262 ◽  
Author(s):  
Ryan Renslow ◽  
Jerome Babauta ◽  
Andrew Kuprat ◽  
Jim Schenk ◽  
Cornelius Ivory ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Extracellular Electron Transfer ◽  
Transfer Mechanisms

scholarly journals Using Structure-Function Relationships to Understand the Mechanism of Phenazine Mediated Extracellular Electron Transfer in Escherichia coli

iScience ◽  
2021 ◽  
pp. 103033
Author(s):  
Zayn Rhodes ◽  
Olja Simoska ◽  
Ashwini Dantanarayana ◽  
Keith J. Stevenson ◽  
Shelley D. Minteer
Keyword(s):  
Escherichia Coli ◽  
Electron Transfer ◽  
Structure Function ◽  
Extracellular Electron Transfer

scholarly journals Ecophysiology of Freshwater Verrucomicrobia Inferred from Metagenome-Assembled Genomes

mSphere ◽  
2017 ◽  
Vol 2 (5) ◽  
Author(s):  
Shaomei He ◽  
Sarah L. R. Stevens ◽  
Leong-Keat Chan ◽  
Stefan Bertilsson ◽  
Tijana Glavina del Rio ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Potential Role ◽  
Carbon Sources ◽  
Genomic Analysis ◽  
Distribution Patterns ◽  
Eutrophic Lake ◽  
Genomic Diversity ◽  
Extracellular Electron Transfer ◽  
Different Origins ◽  
Encoding Genes

ABSTRACT Freshwater Verrucomicrobia spp. are cosmopolitan in lakes and rivers, and yet their roles and ecophysiology are not well understood, as cultured freshwater Verrucomicrobia spp. are restricted to one subdivision of this phylum. Here, we greatly expanded the known genomic diversity of this freshwater lineage by recovering 19 Verrucomicrobia draft genomes from 184 metagenomes collected from a eutrophic lake and a humic bog across multiple years. Most of these genomes represent the first freshwater representatives of several Verrucomicrobia subdivisions. Genomic analysis revealed Verrucomicrobia to be potential (poly)saccharide degraders and suggested their adaptation to carbon sources of different origins in the two contrasting ecosystems. We identified putative extracellular electron transfer genes and so-called “Planctomycete-specific” cytochrome c-encoding genes and identified their distinct distribution patterns between the lakes/layers. Overall, our analysis greatly advances the understanding of the function, ecophysiology, and distribution of freshwater Verrucomicrobia, while highlighting their potential role in freshwater carbon cycling. Microbes are critical in carbon and nutrient cycling in freshwater ecosystems. Members of the Verrucomicrobia are ubiquitous in such systems, and yet their roles and ecophysiology are not well understood. In this study, we recovered 19 Verrucomicrobia draft genomes by sequencing 184 time-series metagenomes from a eutrophic lake and a humic bog that differ in carbon source and nutrient availabilities. These genomes span four of the seven previously defined Verrucomicrobia subdivisions and greatly expand knowledge of the genomic diversity of freshwater Verrucomicrobia. Genome analysis revealed their potential role as (poly)saccharide degraders in freshwater, uncovered interesting genomic features for this lifestyle, and suggested their adaptation to nutrient availabilities in their environments. Verrucomicrobia populations differ significantly between the two lakes in glycoside hydrolase gene abundance and functional profiles, reflecting the autochthonous and terrestrially derived allochthonous carbon sources of the two ecosystems, respectively. Interestingly, a number of genomes recovered from the bog contained gene clusters that potentially encode a novel porin-multiheme cytochrome c complex and might be involved in extracellular electron transfer in the anoxic humus-rich environment. Notably, most epilimnion genomes have large numbers of so-called “Planctomycete-specific” cytochrome c-encoding genes, which exhibited distribution patterns nearly opposite to those seen with glycoside hydrolase genes, probably associated with the different levels of environmental oxygen availability and carbohydrate complexity between lakes/layers. Overall, the recovered genomes represent a major step toward understanding the role, ecophysiology, and distribution of Verrucomicrobia in freshwater. IMPORTANCE Freshwater Verrucomicrobia spp. are cosmopolitan in lakes and rivers, and yet their roles and ecophysiology are not well understood, as cultured freshwater Verrucomicrobia spp. are restricted to one subdivision of this phylum. Here, we greatly expanded the known genomic diversity of this freshwater lineage by recovering 19 Verrucomicrobia draft genomes from 184 metagenomes collected from a eutrophic lake and a humic bog across multiple years. Most of these genomes represent the first freshwater representatives of several Verrucomicrobia subdivisions. Genomic analysis revealed Verrucomicrobia to be potential (poly)saccharide degraders and suggested their adaptation to carbon sources of different origins in the two contrasting ecosystems. We identified putative extracellular electron transfer genes and so-called “Planctomycete-specific” cytochrome c-encoding genes and identified their distinct distribution patterns between the lakes/layers. Overall, our analysis greatly advances the understanding of the function, ecophysiology, and distribution of freshwater Verrucomicrobia, while highlighting their potential role in freshwater carbon cycling.

Geobacter sulfurreducens adapts to low electrode potential for extracellular electron transfer

2016 ◽  
Vol 191 ◽  
pp. 743-749 ◽  
Author(s):  
Luo Peng ◽  
Xiao-Ting Zhang ◽  
Jie Yin ◽  
Shuo-Yuan Xu ◽  
Yong Zhang ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Electrode Potential ◽  
Geobacter Sulfurreducens ◽  
Extracellular Electron Transfer

scholarly journals Long-distance electron transfer in a filamentous Gram-positive bacterium

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Yonggang Yang ◽  
Zegao Wang ◽  
Cuifen Gan ◽  
Lasse Hyldgaard Klausen ◽  
Robin Bonné ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Microbial Fuel Cells ◽  
Extracellular Electron Transfer ◽  
Gram Negative Bacteria ◽  
Positive Bacterium ◽  
Long Distance ◽  
Gram Positive ◽  
Gram Negative ◽  
Force Microscopy ◽  
Cell Envelopes

AbstractLong-distance extracellular electron transfer has been observed in Gram-negative bacteria and plays roles in both natural and engineering processes. The electron transfer can be mediated by conductive protein appendages (in short unicellular bacteria such as Geobacter species) or by conductive cell envelopes (in filamentous multicellular cable bacteria). Here we show that Lysinibacillus varians GY32, a filamentous unicellular Gram-positive bacterium, is capable of bidirectional extracellular electron transfer. In microbial fuel cells, L. varians can form centimetre-range conductive cellular networks and, when grown on graphite electrodes, the cells can reach a remarkable length of 1.08 mm. Atomic force microscopy and microelectrode analyses suggest that the conductivity is linked to pili-like protein appendages. Our results show that long-distance electron transfer is not limited to Gram-negative bacteria.

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