scholarly journals Surfactant Protein B Promotes Cytosolic SiRNA Delivery by Adopting a Virus-like Mechanism of Action

ACS Nano ◽  
2021 ◽  
Author(s):  
Roberta Guagliardo ◽  
Lore Herman ◽  
Jelle Penders ◽  
Agata Zamborlin ◽  
Herlinde De Keersmaecker ◽  
...  
Keyword(s):  
Mechanism Of Action ◽  
Sirna Delivery ◽  
Surfactant Protein ◽  
Surfactant Protein B ◽  
Protein B

scholarly journals Surfactant protein B (SP-B) enhances the cellular siRNA delivery of proteolipid coated nanogels for inhalation therapy

2018 ◽  
Vol 78 ◽  
pp. 236-246 ◽  
Author(s):  
Pieterjan Merckx ◽  
Lynn De Backer ◽  
Lien Van Hoecke ◽  
Roberta Guagliardo ◽  
Mercedes Echaide ◽  
...  
Keyword(s):  
Sirna Delivery ◽  
Surfactant Protein ◽  
Inhalation Therapy ◽  
Surfactant Protein B ◽  
Protein B

scholarly journals Nanocarrier Lipid Composition Modulates the Impact of Pulmonary Surfactant Protein B (SP-B) on Cellular Delivery of siRNA

Pharmaceutics ◽  
2019 ◽  
Vol 11 (9) ◽  
pp. 431 ◽  
Author(s):  
Roberta Guagliardo ◽  
Pieterjan Merckx ◽  
Agata Zamborlin ◽  
Lynn De Backer ◽  
Mercedes Echaide ◽  
...  
Keyword(s):  
Lipid Composition ◽  
Sirna Delivery ◽  
Lung Surfactant ◽  
Surfactant Protein ◽  
Target Cells ◽  
Cellular Delivery ◽  
Major Barrier ◽  
Surfactant Protein B ◽  
The Impact ◽  
Protein B

Two decades since the discovery of the RNA interference (RNAi) pathway, we are now witnessing the approval of the first RNAi-based treatments with small interfering RNA (siRNA) drugs. Nevertheless, the widespread use of siRNA is limited by various extra- and intracellular barriers, requiring its encapsulation in a suitable (nanosized) delivery system. On the intracellular level, the endosomal membrane is a major barrier following endocytosis of siRNA-loaded nanoparticles in target cells and innovative materials to promote cytosolic siRNA delivery are highly sought after. We previously identified the endogenous lung surfactant protein B (SP-B) as siRNA delivery enhancer when reconstituted in (proteo) lipid-coated nanogels. It is known that the surface-active function of SP-B in the lung is influenced by the lipid composition of the lung surfactant. Here, we investigated the role of the lipid component on the siRNA delivery-promoting activity of SP-B proteolipid-coated nanogels in more detail. Our results clearly indicate that SP-B prefers fluid membranes with cholesterol not exceeding physiological levels. In addition, SP-B retains its activity in the presence of different classes of anionic lipids. In contrast, comparable fractions of SP-B did not promote the siRNA delivery potential of DOTAP:DOPE cationic liposomes. Finally, we demonstrate that the beneficial effect of lung surfactant on siRNA delivery is not limited to lung-related cell types, providing broader therapeutic opportunities in other tissues as well.

A model for the structure and mechanism of action of pulmonary surfactant protein B

2015 ◽  
Vol 29 (10) ◽  
pp. 4236-4247 ◽  
Author(s):  
Bárbara Olmeda ◽  
Begoña García‐Álvarez ◽  
Manuel J. Gómez ◽  
Marta Martínez‐Calle ◽  
Antonio Cruz ◽  
...  
Keyword(s):  
Mechanism Of Action ◽  
Pulmonary Surfactant ◽  
Surfactant Protein ◽  
Surfactant Protein B ◽  
Pulmonary Surfactant Protein B ◽  
Pulmonary Surfactant Protein ◽  
Protein B

The pathogenic role of surfactant protein B in formation of bronchopulmonary pathology in children

2017 ◽  
pp. 66-71
Author(s):  
O. Yablon ◽  
◽  
N. Zaichko ◽  
O. Mazulov ◽  
Z.I. Rossokha ◽  
...  
Keyword(s):  
Surfactant Protein ◽  
Pathogenic Role ◽  
Surfactant Protein B ◽  
Protein B

scholarly journals Fetal and neonatal samples of a precursor surfactant protein B inversely related to gestational age

2013 ◽  
Vol 13 (1) ◽  
Author(s):  
Christoph Czernik ◽  
Gerd Schmalisch ◽  
Christoph Bührer ◽  
Hans Proquitté
Keyword(s):  
Gestational Age ◽  
Surfactant Protein ◽  
Surfactant Protein B ◽  
Protein B

scholarly journals Surfactant Protein B Inhibits Endotoxin-Induced Lung Inflammation

2003 ◽  
Vol 28 (3) ◽  
pp. 373-378 ◽  
Author(s):  
Ralph Epaud ◽  
Machiko Ikegami ◽  
Jeffrey A. Whitsett ◽  
Alan H. Jobe ◽  
Timothy E. Weaver ◽  
...  
Keyword(s):  
Lung Inflammation ◽  
Surfactant Protein ◽  
Surfactant Protein B ◽  
Protein B
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