Ligand modification of corpus luteum mitochondrial cytochrome P-450 spectra and cholesterol monooxygenation: an assay of enzyme-specific inhibitors

Biochemistry ◽  
1977 ◽  
Vol 16 (4) ◽  
pp. 593-600 ◽  
Author(s):  
Vytautas I. Uzgiris ◽  
Penelope E. Graves ◽  
Hilton A. Salhanick
Keyword(s):  
Corpus Luteum ◽  
Mitochondrial Cytochrome ◽  
Ligand Modification ◽  
Cytochrome P 450 ◽  
Specific Inhibitors

CYTOCHROME P-450 ACTIVITY IN SHEEP CORPORA LUTEA DURING THE OESTROUS CYCLE

1973 ◽  
Vol 58 (3) ◽  
pp. 493-501 ◽  
Author(s):  
E. R. SIMPSON ◽  
C. R. JEFCOATE ◽  
R. A. COLLETT ◽  
R. B. LAND
Keyword(s):  
Corpus Luteum ◽  
Oestrous Cycle ◽  
Corpora Lutea ◽  
Mitochondrial Cytochrome ◽  
Specific Content ◽  
Substrate Complex ◽  
Chain Cleavage ◽  
Binding Spectra ◽  
Two Parameters ◽  
Cytochrome P 450

SUMMARY Cytochrome P-450 of mitochondria from the ovine corpus luteum was examined on different days throughout the oestrous cycle. Most, if not all, of the mitochondrial cytochrome P-450 appeared to be of the type involved in cholesterol side-chain cleavage, as determined by steroid binding spectra. The content of mitochondrial cytochrome P-450 in the corpus luteum changed considerably throughout the cycle, and closely paralleled the rise and fall of plasma progesterone concentration; the correlation between these two parameters was 0·96. This was largely a reflection of the changes in weight of the corpus luteum throughout the cycle, since the specific content of cytochrome P-450 changed much less dramatically. The percentage of the mitochondrial cytochrome P-450 present as the cholesterol—substrate complex remained constant throughout the cycle at about 25–30% of the total.

scholarly journals 26-Hydroxylation of C27-steroids by soluble liver mitochondrial cytochrome P-450.

1979 ◽  
Vol 254 (14) ◽  
pp. 6464-6469 ◽  
Author(s):  
J I Pedersen ◽  
I Björkhem ◽  
J Gustafsson
Keyword(s):  
Mitochondrial Cytochrome ◽  
Cytochrome P 450
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