On the Electrophilicity of Hydroxyl Radical:  A Laser Flash Photolysis and Computational Study

2005 ◽  
Vol 127 (19) ◽  
pp. 7094-7109 ◽  
Author(s):  
Matthew P. DeMatteo ◽  
James S. Poole ◽  
Xiaofeng Shi ◽  
Rakesh Sachdeva ◽  
Patrick G. Hatcher ◽  
...  
Keyword(s):  
Hydroxyl Radical ◽  
Flash Photolysis ◽  
Laser Flash Photolysis ◽  
Computational Study ◽  
Laser Flash

scholarly journals Intrinsic Buffer Hydroxyl Radical Dosimetry Using Tris(Hydroxymethyl)Aminomethane

2019 ◽  
Author(s):  
Addison E. Roush ◽  
Mohammad Riaz ◽  
Sandeep K. Misra ◽  
Scot R. Weinberger ◽  
Joshua S. Sharp
Keyword(s):  
Hydroxyl Radical ◽  
Real Time ◽  
Hydroxyl Radicals ◽  
Flash Photolysis ◽  
Laser Flash Photolysis ◽  
Laser Flash ◽  
Photochemical Oxidation ◽  
Protein Surfaces ◽  
Radical Production ◽  
Experimental Parameters

AbstractFast Photochemical Oxidation of Proteins (FPOP) is a powerful covalent labeling tool that uses hydroxyl radicals generated by laser flash photolysis of hydrogen peroxide to footprint protein surfaces. Because radical production varies with many experimental parameters, hydroxyl radical dosimeters have been introduced to track the effective radical dosage experienced by the protein analyte. FPOP experiments performed using adenine optical radical dosimetry containing protein in Tris buffer demonstrated unusual dosimetry behavior. We have investigated the behavior of Tris under oxidative conditions in detail. We find that Tris can act as a novel gain-of-signal optical hydroxyl radical dosimeter in FPOP experiments. This new dosimeter is also amenable to inline real-time monitoring thereby allowing real-time adjustments to compensate for differences in samples for their quenching ability.

Laser Flash Photolysis and Computational Study of Singlet Phenylnitrene

1999 ◽  
Vol 121 (6) ◽  
pp. 1202-1207 ◽  
Author(s):  
Nina P. Gritsan ◽  
Zhendong Zhu ◽  
Christopher M. Hadad ◽  
Matthew S. Platz
Keyword(s):  
Flash Photolysis ◽  
Laser Flash Photolysis ◽  
Computational Study ◽  
Laser Flash

Mechanism of Fe(OH)2+(aq) photolysis in aqueous solution(Technical Report)

2000 ◽  
Vol 72 (11) ◽  
pp. 2187-2197 ◽  
Author(s):  
Ivan P. Pozdnyakov ◽  
Evgeny M. Glebov ◽  
Victor F. Plyusnin ◽  
Vyacheslav P. Grivin ◽  
Yuri V. Ivanov ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Hydroxyl Radical ◽  
Flash Photolysis ◽  
Laser Flash Photolysis ◽  
Laser Flash ◽  
Photochemical Process ◽  
Technical Report

Experiments on laser flash photolysis (308 nm) of Fe(OH)2+(aq) complex in aqueous solution with addition of nitrobenzene demonstrate the formation of hydroxyl radical in the primary photochemical process.

scholarly journals A Time-Resolved Study on the Reactivity of Alcoholic Drinks with the Hydroxyl Radical

Molecules ◽  
2019 ◽  
Vol 24 (2) ◽  
pp. 234 ◽  
Author(s):  
Gemma Rodriguez-Muñiz ◽  
Miguel Miranda ◽  
M. Marin
Keyword(s):  
Hydroxyl Radical ◽  
Flash Photolysis ◽  
Laser Flash Photolysis ◽  
Laser Flash ◽  
White Wine ◽  
Alcoholic Beverages ◽  
Quenching Rate ◽  
Time Resolved ◽  
Quenching Rate Constant ◽  
Different Origins

Reactive oxygen species (ROS) can provoke damage to cells, where their concentrations are regulated by antioxidants. As the hydroxyl radical (•OH) is the most oxidizing ROS, we have focused our attention on the use of a mechanistically based time-resolved methodology, such as laser flash photolysis, to determine the relative reactivity of alcoholic beverages towards •OH as an indicator of their antioxidant potential. The selected drinks were of two different origins: (i) those derived from grapes such as red wine, white wine, white vermouth, marc and brandy and (ii) spirits not derived from grapes: triple sec, gin, whisky, and rum. Initially, we determined the quenching rate constant of ethanol with •OH and then we explored the reactivity of the different beverages, which was higher than expected based on their alcoholic content. This can be attributed to the presence of antioxidants and was especially remarkable for the grape-derived drinks.

Kinetic studies of the reaction between pesticides and hydroxyl radical generated by laser flash photolysis

2015 ◽  
Vol 96 (5) ◽  
pp. 1580-1584 ◽  
Author(s):  
Fábio Gozzi ◽  
Silvio C Oliveira ◽  
Renato F Dantas ◽  
Volnir O Silva ◽  
Frank H Quina ◽  
...  
Keyword(s):  
Hydroxyl Radical ◽  
Flash Photolysis ◽  
Laser Flash Photolysis ◽  
Kinetic Studies ◽  
Laser Flash

A Matrix Isolation, Laser Flash Photolysis, and Computational Study of Adamantene

2001 ◽  
Vol 105 (15) ◽  
pp. 3803-3807 ◽  
Author(s):  
Eunju Lee Tae ◽  
Zhendong Zhu ◽  
Matthew S. Platz
Keyword(s):  
Flash Photolysis ◽  
Laser Flash Photolysis ◽  
Computational Study ◽  
Matrix Isolation ◽  
Laser Flash

scholarly journals Photodegradation of organic pollutants in aqueous solutions caused by Fe(OH)aq2+ photolysis: Evidence of OH radical formation

2004 ◽  
Vol 6 (2) ◽  
pp. 89-93 ◽  
Author(s):  
I. P. Pozdnyakov ◽  
Yu. A. Sosedova ◽  
V. F. Plyusnin ◽  
E. M. Glebov ◽  
V. P. Grivin ◽  
...  
Keyword(s):  
Hydroxyl Radical ◽  
Flash Photolysis ◽  
Laser Flash Photolysis ◽  
Laser Flash ◽  
Photochemical Reactions ◽  
Radical Formation ◽  
Nanosecond Laser ◽  
Alternative Mechanism ◽  
Organic Additives ◽  
Intermolecular Electron Transfer

Photochemistry of Fe(III) hydroxocomplexes has a great interest because of possibility of hydroxyl radical formation at the primary photochemical step. This mechanism is based mainly on the analysis of the final products of photochemical reactions between light-excited Fe(III) complexes and organic compounds. However, realization of an alternative mechanism of photolysis, based on the intermolecular electron transfer from organic molecules located in the second coordination sphere to the light-excited complex could take place. To distinguish these mechanisms, the experiments on the nanosecond laser flash photolysis of Fe(OH)aq2+ in water solutions (pH 3) with organic additives; namely nitrobenzene, N,N-dimethylformamide, and phenol have been performed. And the formation of the organic radicals Ph(•OH)NO2, HCON(CH2•)(CH3), and Ph(OH)2•, was observed, respectively. The rate constants of the formation and decay of the radicals coincide with those found in literature. The results support the formation of the hydroxyl radical upon the photolysis of Fe(OH)aq2+.

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