In vivo digestibility of bean (Phaseolus vulgaris L.) proteins: the role of endogenous protein

Background: α-synuclein (α-syn) aggregation contributes to the progression of multiple neurodegenerative diseases. We recently found that the isoform b of the co-chaperone DNAJB6 is a strong suppressor of a-syn aggregation in vivo and in vitro. However, nothing is known about the role of the endogenous isoform b of DNAJB6 (DNAJB6b) in health and disease, due to lack of specific antibodies. Objective: Here we generated a novel anti-DNAJB6b antibody to analyze the localization and expression this isoform in cells, in tissue and in clinical material. Methods: To address this we used immunocytochemistry, immunohistochemistry, as well as a novel quantitative DNAJB6 specific ELISA method. Results: The endogenous protein is mainly expressed in the cytoplasm and in neurites in vitro, where it is found more in dendrites than in axons. We further verified in vivo that DNAJB6b is expressed in the dopaminergic neurons of the substantia nigra pars compacta (SNpc), which is a neuronal subpopulation highly sensitive to α-syn aggregation, that degenerate to a large extend in patients with Parkinson’s disease (PD) and multiple system atrophy (MSA). When we analyzed the expression levels of DNAJB6b in brain material from PD and MSA patients, we found a downregulation of DNAJB6b by use of ELISA based quantification. Interestingly, this was also true when analyzing tissue from patients with progressive supranuclear palsy, a taupathic atypical parkinsonian disorder. However, the total level of DNAJB6 was upregulated in these three diseases, which may indicate an upregulation of the other major isoform of DNAJB6, DNAJB6a. Conclusion: This study shows that DNAJB6b is downregulated in several different neurodegenerative diseases, which makes it an interesting target to further investigate in relation to amyloid protein aggregation and disease progression.

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Soil Application of Effective Microorganisms (EM) Maintains Leaf Photosynthetic Efficiency, Increases Seed Yield and Quality Traits of Bean (Phaseolus vulgaris L.) Plants Grown on Different Substrates

International Journal of Molecular Sciences ◽

10.3390/ijms20092327 ◽

2019 ◽

Vol 20 (9) ◽

pp. 2327 ◽

Cited By ~ 3

Author(s):

Marcello Iriti ◽

Alessio Scarafoni ◽

Simon Pierce ◽

Giulia Castorina ◽

Sara Vitalini

Keyword(s):

Phaseolus Vulgaris ◽

Photosystem Ii ◽

Sandy Soil ◽

Photosynthetic Efficiency ◽

Quality Traits ◽

Phaseolus Vulgaris L ◽

Effective Microorganisms ◽

Yield And Quality ◽

Bean Plants

EM (effective microorganisms) is a biofertilizer consisting of a mixed culture of potentially beneficial microorganisms. In this study, we investigated the effects of EM treatment on leaf in vivo chlorophyll a fluorescence of photosystem II (PSII), yield, and macronutrient content of bean plants grown on different substrates (nutrient rich substrate vs. nutrient poor sandy soil) in controlled environmental conditions (pot experiment in greenhouse). EM-treated plants maintained optimum leaf photosynthetic efficiency two weeks longer than the control plants, and increased yield independent of substrate. The levels of seed nutritionally-relevant molecules (proteins, lipids, and starch) were only slightly modified, apart from the protein content, which increased in plants grown in sandy soil. Although EM can be considered a promising and environmentally friendly technology for sustainable agriculture, more studies are needed to elucidate the mechanism(s) of action of EM, as well as its efficacy under open field conditions.

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Características fotossintéticas de Phaseolus vulgaris L.

Hoehnea ◽

10.1590/s2236-89062011000200007 ◽

2011 ◽

Vol 38 (2) ◽

pp. 273-280 ◽

Cited By ~ 1

Author(s):

Carlos Pimentel ◽

Ricardo Ferraz de Oliveira ◽

Rafael Vasconcelos Ribeiro ◽

Mauro Guida dos Santos ◽

Eduardo Caruso Machado

Keyword(s):

Phaseolus Vulgaris ◽

Phaseolus Vulgaris L

A análise in vivo da taxa de assimilação líquida de CO2 (A) em resposta à densidade de fluxo de fótons fotossinteticamente ativos (DFFFA) e em resposta à concentração intercelular de CO2 da folha (Ci) permite uma avaliação detalhada do processo fotossintético. Com as curvas A/DFFFA, se estabelece a DFFFA máxima, em que a maior parte da energia absorvida é transferida para a formação de produtos fotoquímicos. Já com as curvas A/Ci, calculou-se a velocidade máxima de carboxilação da Rubisco (Vc,max) de 89,3 µmol m-2 s-1, a velocidade máxima de oxigenação da Rubisco (Vo,max) de 24,7 µmol m-2 s-1, a velocidade máxima de transporte de elétrons (Jmax) de 159,8 µmol m-2 s-1, do qual a regeneração de RuBP é dependente, a respiração mitocondrial (Rd) de 0,88 µmol m-2 s-1, e a condutância mesofílica (g m) de 0,47 mol m-2 s-1, para o feijoeiro comum cultivado no Brasil (Phaseolus vulgaris var. mexicanus).

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The Effect of Decreasing Temperature up to Chilling Values on the in vivo F685/F735 Chlorophyll Fluorescence Ratio in Phaseolus vulgaris and Pisum sativum: The Role of the Photosystem I Contribution to the 735 nm Fluorescence Band ¶

Photochemistry and Photobiology ◽

10.1562/0031-8655(2000)0720075teodtu2.0.co2 ◽

2007 ◽

Vol 72 (1) ◽

pp. 75-84 ◽

Cited By ~ 2

Author(s):

Giovanni Agati ◽

Zoran G. Cerovic ◽

Ismaël Moya

Keyword(s):

Chlorophyll Fluorescence ◽

Phaseolus Vulgaris ◽

Pisum Sativum ◽

Photosystem I ◽

Fluorescence Band ◽

Fluorescence Ratio

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Baked corn (Zea mays L.) and cooked common bean (Phaseolus vulgaris L.) chips improved enzymatic biomarkers and alleviated inflammation during chronic colitis in vivo (P06-063-19)

Current Developments in Nutrition ◽

10.1093/cdn/nzz031.p06-063-19 ◽

2019 ◽

Vol 3 (Supplement_1) ◽

Author(s):

Ivan Luzardo ◽

Rocio Campos-Vega ◽

Elvira Gonzalez de Mejia ◽

Flavia Loarca

Keyword(s):

Zea Mays ◽

Phaseolus Vulgaris ◽

Common Bean ◽

Zea Mays L ◽

Relative Weight ◽

Inflammatory Cells ◽

Phaseolus Vulgaris L ◽

Chronic Colitis ◽

Gut Barrier Function

Abstract Objectives The aim of this research was to evaluate the anti-inflammatory properties of an oven-baked nixtamalized corn (Zea mays L.)/cooked common bean (Phaseolus vulgaris L.) chip in a model of chronic colitis in vivo. The hypothesis was that the chip consumption would prevent colon barrier disruption and improve enzymatic biomarkers by the modulation of infiltration and adhesion of inflammatory cells. Methods A 70% corn and 30% bean chip (7030C) was evaluated, using dextran sodium sulfate (DSS, 2% v/v) as chemical inductor of colitis. After 1-week of acclimatization, 36 CD-1 male mice (6–8 weeks age) were randomly divided into 5 groups: G1 (negative control, fed with basal diet, BD, and water); G2 (positive control, BD + DSS), G3 (100 g 7030C/kg body weight, BW/day + BD), G4 (200 g 7030C/kg BW + BD) and G5 (300 g 7030C/kg BW + BS). The G2-G5 groups were administered DSS every other week, during 5-weeks.After the mice were euthanized, BW and disease activity index (DAI) were recorded. Liver, colon, and spleen were collected, weighed and analyzed for histology. Colonic myeloperoxidase (MPO)/fecal b-glucuronidase (GLUC) activities were also quantified, as well as fecal/cecal metabolites. The colonic mRNA expression of inflammation-associated genes was conducted using a gene inflammation profiler array. Results DSS increased DAI up to 2 units, BW loss was 10–17%, and induced colon shortening 10–15%. Compared to G2, G4 exhibited significantly (p < 0.05) lower DAI (0.75 ± 0.01), spleen relative weight (0.003 ± 0.0001) and colon weight/length ratio (0.045 ± 0.008). The histological analysis showed that the chip consumption prevented colonic barrier damage. G4 displayed the lowest MPO and GLUC among all DSS-induced groups (0.004 ± 0.0004 mU/mg colon; 0.44 ± 0.01 mmol/min/g feces, respectively), and the lowest seric content of MCP-1 protein. Amid all the quantified metabolites, the chip consumption significantly reduced the fecal/cecal content of acetic acid, while butyric and propionic increased at the end of the study. Inflammation gene expression was modulated by the chip consumption. Conclusions Our results suggest that the consumption of this chip might alleviate chronic colitis symptoms because of a protective effect in the gut barrier function and the modulation of infiltration of inflammatory cells. Funding Sources The funding received by CONACyT, CONCyTEQ-Mexico and NIFA-USDA-HATCH are appreciated.

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