Combined Use of Atomic Force Microscopy, X-ray Photoelectron Spectroscopy, and Secondary Ion Mass Spectrometry for Cell Surface Analysis

Langmuir ◽  
2008 ◽  
Vol 24 (7) ◽  
pp. 2955-2959 ◽  
Author(s):  
Etienne Dague ◽  
Arnaud Delcorte ◽  
Jean-Paul Latgé ◽  
Yves F. Dufrêne
Keyword(s):  
Mass Spectrometry ◽  
Atomic Force Microscopy ◽  
Cell Surface ◽  
Photoelectron Spectroscopy ◽  
Secondary Ion Mass Spectrometry ◽  
X Ray ◽  
Force Microscopy ◽  
Atomic Force ◽  
Combined Use ◽  
Secondary Ion

Cleanability of Soiled Stainless Steel as Studied by Atomic Force Microscopy and Time of Flight Secondary Ion Mass Spectrometry

2001 ◽  
Vol 64 (1) ◽  
pp. 87-93 ◽  
Author(s):  
R. D. BOYD ◽  
D. COLE ◽  
D. ROWE ◽  
J. VERRAN ◽  
A. J. PAUL ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Atomic Force Microscopy ◽  
Stainless Steel ◽  
Surface Defects ◽  
Secondary Ion Mass Spectrometry ◽  
Time Of Flight ◽  
Analytical Techniques ◽  
Force Microscopy ◽  
Atomic Force ◽  
Secondary Ion

The hygienic status of food contact surfaces can deteriorate with wear. Effective cleaning regimes must remove any adsorbed organic material as well as microorganisms. Previous work has determined the extent of surface wear occurring on a stainless steel surface within the food industry, and we have reproduced representative samples in vitro. Two surface analytical techniques, atomic force microscopy and time of flight secondary ion mass spectrometry were combined with fluorescence microscopy to give detailed analysis of stainless steel surfaces fouled with starch and milk powder, then cleaned with water either by a spray or brushing method. It was found that the surface cleanability is affected by the cleaning regime and the surface roughness, not only the average vertical roughness but also by the shape of the surface defects, with sharp scratches more difficult to clean than wider surface defects. Spray cleaning with distilled water was found to be a selective method by preferentially removing proteinaceous material more easily than fatty acid ester material. The analytical techniques employed provided information on selective cleanability and surface topography at a hitherto unexplored level, and the information gained may be of value in the design and investigation of novel cleaning regimes and hygienic surfaces.

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