Failure to corroborate claims that the developing endosperm of wheat (Triticum aestivum) contains significant activities of fructose-1,6-bisphosphatase

A RFLP map of rye, Secale cereale, chromosome 7R was constructed using 22 DNA probes including genes coding for leaf acyl carrier protein III, chloroplast fructose-1,6-bisphosphatase, α-amylase-2, EmBP-1, and a peroxidase. All probes had previously been located in wheat, Triticum aestivum, and a comparison of the wheat and rye maps reveals that chromosome 7R contains four segments that are homologous with segments of chromosomes of four homoeologous groups of wheat, 5L, 4L, 7S and 7L including the centromere, and 2S. Within these segments the rye and wheat maps are colinear and orientated in the same way relative to the centromere. The implications of rearrangements such as detected in 7R for triticale breeding and interspecific chromosome recombination and manipulation are discussed.Key words: rye, Secale cereale, RFLP, genetic map, chromosome 7R, colinearity.

Download Full-text

Failure to corroborate claims that the developing endosperm of wheat (Triticum aestivum) contains significant activities of fructose-1,6-bisphosphatase

Physiologia Plantarum ◽

10.1111/j.1399-3054.1990.tb00037.x ◽

1990 ◽

Vol 79 (4) ◽

pp. 635-640 ◽

Cited By ~ 2

Author(s):

Gail Entwistle ◽

Tom Rees

Keyword(s):

Triticum Aestivum ◽

Fructose 1,6 Bisphosphatase

Download Full-text

Enzymic capacities of amyloplasts from wheat (Triticum aestivum) endosperm

Biochemical Journal ◽

10.1042/bj2550391 ◽

1988 ◽

Vol 255 (2) ◽

pp. 391-396 ◽

Cited By ~ 46

Author(s):

G Entwistle ◽

T A Rees

Keyword(s):

Triticum Aestivum ◽

Significant Proportion ◽

Starch Synthesis ◽

Immunoblot Analysis ◽

Glycolytic Enzyme ◽

Starch Synthase ◽

Wheat Endosperm ◽

Fructose 1,6 Bisphosphatase ◽

Enzyme Distribution ◽

Adp Glucose Pyrophosphorylase

Lysates of protoplasts from the endosperm of developing grains of wheat (Triticum aestivum) were fractionated on density gradients of Nycodenz to give amyloplasts. Enzyme distribution on the gradients suggested that: (i) starch synthase and ADP-glucose pyrophosphorylase are confined to the amyloplasts; (ii) pyrophosphate: fructose-6-phosphate 1-phosphotransferase and UDP-glucose pyrophosphorylase are confined to the cytosol; (iii) a significant proportion (23-45%) of each glycolytic enzyme, from phosphoglucomutase to pyruvate kinase inclusive, is in the amyloplast. Starch synthase, ADP-glucose pyrophosphorylase and each of the glycolytic enzymes showed appreciable latency when assayed in unfractionated lysates of protoplasts. No activity of fructose-1,6-bisphosphatase was found in amyloplasts or in homogenates of endosperm. Antibody to plastidic fructose-1,6-bisphosphatase did not react positively, in an immunoblot analysis, with any protein in extracts of wheat endosperm. It is argued that wheat endosperm lacks significant plastidic fructose-1,6-bisphosphatase and that carbon for starch synthesis does not enter the amyloplast as a C-3 compound but probably as hexose phosphate.

Download Full-text

Ultrastructure of Plant Leaf Tissue Infected with Mite-Borne Viral-Like Pathogens

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100067881 ◽

1970 ◽

Vol 28 ◽

pp. 178-179 ◽

Cited By ~ 2

Author(s):

O. E. Bradfute ◽

R. E. Whitmoyer ◽

L. R. Nault

Keyword(s):

Triticum Aestivum ◽

Zea Mays ◽

Electron Microscope ◽

Hordeum Vulgare ◽

Microscope Study ◽

Electron Microscope Study ◽

Leaf Tissue ◽

Leaf Ultrastructure ◽

Double Membrane ◽

Aceria Tulipae

A pathogen transmitted by the eriophyid mite, Aceria tulipae, infects a number of Gramineae producing symptoms similar to wheat spot mosaic virus (1). An electron microscope study of leaf ultrastructure from systemically infected Zea mays, Hordeum vulgare, and Triticum aestivum showed the presence of ovoid, double membrane bodies (0.1 - 0.2 microns) in the cytoplasm of parenchyma, phloem and epidermis cells (Fig. 1 ).

Download Full-text