scholarly journals Organizational principles of amygdalar input-output neuronal circuits

2021 ◽  
Author(s):  
Limeng Huang ◽  
Yiwen Chen ◽  
Sen Jin ◽  
Li Lin ◽  
Shumin Duan ◽  
...  
Keyword(s):  
Brain Structures ◽  
Neuronal Circuits ◽  
Input Output ◽  
Cell Type ◽  
Bed Nucleus ◽  
Efferent Neurons ◽  
Transsynaptic Tracing ◽  
Objective Basis ◽  
Basolateral Complex ◽  
Neuronal Groups

AbstractThe amygdala, one of the most studied brain structures, integrates brain-wide heterogeneous inputs and governs multidimensional outputs to control diverse behaviors central to survival, yet how amygdalar input-output neuronal circuits are organized remains unclear. Using a simplified cell-type- and projection-specific retrograde transsynaptic tracing technique, we scrutinized brain-wide afferent inputs of four major output neuronal groups in the amygdalar basolateral complex (BLA) that project to the bed nucleus of the stria terminals (BNST), ventral hippocampus (vHPC), medial prefrontal cortex (mPFC) and nucleus accumbens (NAc), respectively. Brain-wide input-output quantitative analysis unveils that BLA efferent neurons receive a diverse array of afferents with varied input weights and predominant contextual representation. Notably, the afferents received by BNST-, vHPC-, mPFC- and NAc-projecting BLA neurons exhibit virtually identical origins and input weights. These results indicate that the organization of amygdalar BLA input-output neuronal circuits follows the input-dependent and output-independent principles, ideal for integrating brain-wide diverse afferent stimuli to control parallel efferent actions. The data provide the objective basis for improving the virtual reality exposure therapy for anxiety disorders and validate the simplified cell-type- and projection-specific retrograde transsynaptic tracing method.

scholarly journals Divergent neural pathways emanating from the lateral parabrachial nucleus mediate distinct components of the pain response

2019 ◽  
Author(s):  
Michael C. Chiang ◽  
Eileen K. Nguyen ◽  
Andrew E. Papale ◽  
Sarah E. Ross
Keyword(s):  
Ventromedial Hypothalamus ◽  
Parabrachial Nucleus ◽  
Projection Neurons ◽  
Pain Response ◽  
Neural Pathways ◽  
Bed Nucleus ◽  
Lateral Parabrachial Nucleus ◽  
Efferent Projections ◽  
Efferent Neurons ◽  
Nociceptive Input

ABSTRACTThe lateral parabrachial nucleus (lPBN) is a major target of spinal projection neurons conveying nociceptive input into supraspinal structures. However, the functional role of distinct lPBN efferents for diverse nocifensive responses have remained largely uncharacterized. Here, we show that two populations of efferent neurons from different regions of the lPBN collateralize to distinct targets. Activation of efferent projections to the ventromedial hypothalamus (VMH) or lateral periaqueductal gray (lPAG) drive escape behaviors, whereas the activation of lPBN efferents to the bed nucleus stria terminalis (BNST) or central amygdala (CEA) generates an aversive memory. Finally, we provide evidence that dynorphin expressing neurons span cytoarchitecturally distinct domains of the lPBN to coordinate these distinct aspects of the nocifensive response.HIGHLIGHTSSpatially segregated neurons in the lPBN collateralize to distinct targets.Distinct output pathways give rise to separate aspects of the pain response.Dynorphin neurons within the lPBN convey noxious information across subdivisions.eTOC BLURBChiang et al. reveal that neurons in spatially segregated regions of the lateral parabrachial nucleus collateralize to distinct targets, and that activation of distinct efferents gives rise to separate components of the nocifensive response.

scholarly journals Chronic stress induces cell type-selective transcriptomic and electrophysiological changes in the bed nucleus of the stria terminalis

2019 ◽  
Vol 150 ◽  
pp. 80-90 ◽  
Author(s):  
Sarah E. Daniel ◽  
Aurélie Menigoz ◽  
Jidong Guo ◽  
Steven J. Ryan ◽  
Shivani Seth ◽  
...  
Keyword(s):  
Chronic Stress ◽  
Stria Terminalis ◽  
Cell Type ◽  
Bed Nucleus

scholarly journals L1CAM/Neuroglian controls the axon–axon interactions establishing layered and lobular mushroom body architecture

2015 ◽  
Vol 208 (7) ◽  
pp. 1003-1018 ◽  
Author(s):  
Dominique Siegenthaler ◽  
Eva-Maria Enneking ◽  
Eliza Moreno ◽  
Jan Pielage
Keyword(s):  
Cell Adhesion ◽  
Mushroom Body ◽  
Cell Adhesion Molecule ◽  
Cluster Formation ◽  
Neuronal Circuits ◽  
Cell Type ◽  
Cell Type Specific ◽  
And Control ◽  
Homophilic Adhesion

The establishment of neuronal circuits depends on the guidance of axons both along and in between axonal populations of different identity; however, the molecular principles controlling axon–axon interactions in vivo remain largely elusive. We demonstrate that the Drosophila melanogaster L1CAM homologue Neuroglian mediates adhesion between functionally distinct mushroom body axon populations to enforce and control appropriate projections into distinct axonal layers and lobes essential for olfactory learning and memory. We addressed the regulatory mechanisms controlling homophilic Neuroglian-mediated cell adhesion by analyzing targeted mutations of extra- and intracellular Neuroglian domains in combination with cell type–specific rescue assays in vivo. We demonstrate independent and cooperative domain requirements: intercalating growth depends on homophilic adhesion mediated by extracellular Ig domains. For functional cluster formation, intracellular Ankyrin2 association is sufficient on one side of the trans-axonal complex whereas Moesin association is likely required simultaneously in both interacting axonal populations. Together, our results provide novel mechanistic insights into cell adhesion molecule–mediated axon–axon interactions that enable precise assembly of complex neuronal circuits.

scholarly journals Opiate dependence induces cell type-specific plasticity of intrinsic membrane properties in the rat juxtacapsular bed nucleus of stria terminalis (jcBNST)

2017 ◽  
Vol 234 (23-24) ◽  
pp. 3485-3498 ◽  
Author(s):  
Walter Francesconi ◽  
Attila Szücs ◽  
Fulvia Berton ◽  
George F. Koob ◽  
Leandro F. Vendruscolo ◽  
...  
Keyword(s):  
Opiate Dependence ◽  
Membrane Properties ◽  
Stria Terminalis ◽  
Cell Type ◽  
Bed Nucleus ◽  
Cell Type Specific ◽  
Intrinsic Membrane Properties

Cellular Physiology of the Basolateral Complex of the Amygdala and Its Modulation by Stress

2016 ◽  
Author(s):  
Brendan M. O’Flaherty ◽  
Chia-Chun Hsu ◽  
M. Anzar Abbas ◽  
Donald G. Rainnie
Keyword(s):  
Traumatic Event ◽  
Network Connectivity ◽  
The United States ◽  
Brain Regions ◽  
Neural Systems ◽  
Fear Response ◽  
Post Traumatic Stress ◽  
Bed Nucleus ◽  
Post Traumatic ◽  
Basolateral Complex

Fear is a critical emotional response that allows an organism to safely navigate through dangerous environments. The neural systems underlying the fear response have been well characterized, and include the amygdala, hippocampus, prefrontal cortex, bed nucleus of stria terminalis, nucleus accumbens, and others. While normally these brain regions coordinate to produce an appropriate fear response, the fear network in humans can become dysregulated after a traumatic event. The resulting phenotype of hyperarousal, avoidance, and re-experiencing of fear known as post-traumatic stress disorder (PTSD) is a growing problem in the United States. This chapter focuses on the role of the basolateral complex (BLC) of the amygdala, which has been implicated in the neuropathology of PTSD, particularly the hyperarousal, fear generalization, and fear extinction deficits characteristic of the disorder, as well as aspects of the microcircuitry, network connectivity, and neuromodulation of the BLC that may be involved in the pathophysiology of PTSD.

Cell-type diversity in the bed nucleus of the stria terminalis to regulate motivated behaviors

2021 ◽  
pp. 113401
Author(s):  
Maria M. Ortiz-Juza ◽  
Rizk A. Alghorazi ◽  
Jose Rodriguez-Romaguera
Keyword(s):  
Stria Terminalis ◽  
Cell Type ◽  
Bed Nucleus ◽  
Motivated Behaviors
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