scholarly journals Structure of the human frataxin-bound iron-sulfur cluster assembly complex provides insight into its activation mechanism

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Nicholas G. Fox ◽  
Xiaodi Yu ◽  
Xidong Feng ◽  
Henry J. Bailey ◽  
Alain Martelli ◽  
...  
Keyword(s):  
Activation Mechanism ◽  
Cluster Assembly ◽  
Iron Sulfur Cluster ◽  
Sulfur Cluster ◽  
Iron Sulfur ◽  
Insight Into

scholarly journals Multiple Sense and Antisense Promoters Contribute to the Regulated Expression of the isc-suf Operon for Iron-Sulfur Cluster Assembly in Rhodobacter

2019 ◽  
Vol 7 (12) ◽  
pp. 671 ◽  
Author(s):  
Xin Nie ◽  
Bernhard Remes ◽  
Gabriele Klug
Keyword(s):  
Rhodobacter Sphaeroides ◽  
Photosynthetic Electron Transport ◽  
Regulatory Proteins ◽  
Cluster Assembly ◽  
Iron Sulfur Cluster ◽  
Iron Sulfur Clusters ◽  
Sulfur Cluster ◽  
Iron Sulfur ◽  
Photosynthetic Complexes ◽  
The One

A multitude of biological functions relies on iron-sulfur clusters. The formation of photosynthetic complexes goes along with an additional demand for iron-sulfur clusters for bacteriochlorophyll synthesis and photosynthetic electron transport. However, photooxidative stress leads to the destruction of iron-sulfur clusters, and the released iron promotes the formation of further reactive oxygen species. A balanced regulation of iron-sulfur cluster synthesis is required to guarantee the supply of this cofactor, on the one hand, but also to limit stress, on the other hand. The phototrophic alpha-proteobacterium Rhodobacter sphaeroides harbors a large operon for iron-sulfur cluster assembly comprising the iscRS and suf genes. IscR (iron-sulfur cluster regulator) is an iron-dependent regulator of isc-suf genes and other genes with a role in iron metabolism. We applied reporter gene fusions to identify promoters of the isc-suf operon and studied their activity alone or in combination under different conditions. Gel-retardation assays showed the binding of regulatory proteins to individual promoters. Our results demonstrated that several promoters in a sense and antisense direction influenced isc-suf expression and the binding of the IscR, Irr, and OxyR regulatory proteins to individual promoters. These findings demonstrated a complex regulatory network of several promoters and regulatory proteins that helped to adjust iron-sulfur cluster assembly to changing conditions in Rhodobacter sphaeroides.

scholarly journals Regulation of the HscA ATPase Reaction Cycle by the Co-chaperone HscB and the Iron-Sulfur Cluster Assembly Protein IscU

2004 ◽  
Vol 279 (52) ◽  
pp. 53924-53931 ◽  
Author(s):  
Jonathan J. Silberg ◽  
Tim L. Tapley ◽  
Kevin G. Hoff ◽  
Larry E. Vickery
Keyword(s):  
Cluster Assembly ◽  
Iron Sulfur Cluster ◽  
Reaction Cycle ◽  
Sulfur Cluster ◽  
Iron Sulfur ◽  
Atpase Reaction

scholarly journals Glutaredoxin GRXS17 Associates with the Cytosolic Iron-Sulfur Cluster Assembly Pathway

2016 ◽  
pp. pp.00261.2016 ◽  
Author(s):  
Sabrina Inigo ◽  
Astrid Nagels Durand ◽  
Andres Ritter ◽  
Sabine Le Gall ◽  
Martin Termathe ◽  
...  
Keyword(s):  
Cluster Assembly ◽  
Iron Sulfur Cluster ◽  
Sulfur Cluster ◽  
Iron Sulfur ◽  
Assembly Pathway

scholarly journals Multiple Turnover Transfer of [2Fe2S] Clusters by the Iron-Sulfur Cluster Assembly Scaffold Proteins IscU and IscA

2005 ◽  
Vol 280 (33) ◽  
pp. 29513-29518 ◽  
Author(s):  
Francesco Bonomi ◽  
Stefania Iametti ◽  
Dennis Ta ◽  
Larry E. Vickery
Keyword(s):  
Scaffold Proteins ◽  
Cluster Assembly ◽  
Iron Sulfur Cluster ◽  
Sulfur Cluster ◽  
Iron Sulfur
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