Elevation of soybean seed oil content through selection for seed coat shininess

Soybean is one of the most important oil crops in the world. Revealing the molecular basis and exploring key candidate genes for seed oil synthesis has great significance for soybean improvement. In this study, we found that oil accumulation rates and gene expression levels changed dynamically during soybean seed development. The expression levels of genes in metabolic pathways such as carbon fixation, photosynthesis, glycolysis, and fatty acid biosynthesis were significantly up-regulated during the rapid accumulation of oil in developing soybean seeds. Through weighted correlation network analysis, we identified six co-expression modules associated with soybean seed oil content and the pink module was the most positively correlated (r = 0.83, p = 7 × 10−4) network. Through the integration of differential expression and co-expression analysis, we predicted 124 candidate genes potentially affecting soybean seed oil content, including seven genes in lipid metabolism pathway, two genes involved in glycolysis, one gene in sucrose metabolism, and 12 genes belonged to transcription factors as well as other categories. Among these, three genes (GmABI3b, GmNFYA and GmFAD2-1B) have been shown to control oil and fatty acid content in soybean seeds, and other newly identified candidate genes would broaden our knowledge to understand the molecular basis for oil accumulation in soybean seeds.

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Natural variation and selection in GmSWEET39 affect soybean seed oil content

New Phytologist ◽

10.1111/nph.16250 ◽

2019 ◽

Vol 225 (4) ◽

pp. 1651-1666 ◽

Cited By ~ 7

Author(s):

Long Miao ◽

Songnan Yang ◽

Kai Zhang ◽

Jianbo He ◽

Chunhua Wu ◽

...

Keyword(s):

Natural Variation ◽

Oil Content ◽

Seed Oil ◽

Soybean Seed ◽

Seed Oil Content ◽

Variation And Selection

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Identification of additive and epistatic effects QTLs for seed oil content in soybean based on an integrating map of two RIL populations

10.21203/rs.3.rs-39376/v1 ◽

2020 ◽

Author(s):

Yue Wang ◽

Shulin Liu ◽

Jiajing Wang ◽

Chang Yang ◽

Zhixi Tian ◽

...

Keyword(s):

Oil Content ◽

Seed Oil ◽

Fatty Acid Biosynthesis ◽

Soybean Seed ◽

Additive Effect ◽

Human Consumption ◽

Environment Interaction ◽

Potential Candidate ◽

Seed Oil Content ◽

Epistatic Effects

Abstract Background Soybean seed oil has been widely used in human consumption and industrial production. Results In order to identify the additive and epistatic effects QTLs and QTLs by environments interactions (AE and AAE) for seed oil content in soybean, an eight-environment conjoint analysis based on two populations RIL3613 and RIL6013 with an integrating map was conducted. An new high-density integrated genetic map containing 2212 SNP markers and covering 5718.01 cM with an average distance of 2.61 cM were constructed by the combination of two linkage maps of two associated recombinant inbred line (A-RIL) populations. A total of 64 additive effect and additive × environment interaction (AE) QTL were identified on 19 chromosomes by both ICIM and IM methods, and the proportion of phenotypic variations explained (PVE) range of QTL related to oil content was 1.29–10.75%, of which 19 QTLs had overlapping marker intervals, and qOil-5-1 was identified simultaneously in both RIL populations. Compared with previous SSR positioning results, it is found 8 SNP sites within the QTL physical interval located in the SSR sites. Among them, 4 QTLs were new found. Twelve pairs of epistatic QTLs (additive × additive, AA) and QTL interactions with environments (AAE) for oil content were identified by the ICIM method, of which 3 QTLs were new found, and 2 additive effect QTLs, qOil-9-2 and qOil-15-1, linked to the other two QTLs to produce epistatic effects. A total of 5 potential candidate genes were identified based on genetic ontology and annotated information showing the relationship with seed oil content and/or fatty acid biosynthesis and metabolism. Conclusion These QTLs with different effects provide the good basis for molecular-assisted breeding of soybean oil content-related traits and further fine mapping of related genes.

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Soybean seed oil content: genetic control under different photoperiods

Genetics and Molecular Biology ◽

10.1590/s1415-47571998000300017 ◽

1998 ◽

Vol 21 (3) ◽

pp. 387-394 ◽

Cited By ~ 3

Author(s):

Zilda F.S. Miranda ◽

Carlos A. Arrabal Arias ◽

José Francisco Ferraz de Toledo ◽

Marcelo Fernandes de Oliveira

Keyword(s):

Oil Content ◽

Seed Oil ◽

Additive Genetic Variance ◽

Soybean Seed ◽

Sowing Date ◽

Inbred Lines ◽

Seed Oil Content ◽

Additive Interaction ◽

Additive Variance ◽

Sowing Dates

The oil content of soybean (Glycine max (L.) Merrill) seeds is a polygenic and complex trait that is responsive to environmental effects that occur during plant development. Our objective was to study the seed oil content of soybeans developed under diverse photoperiod and temperature conditions. Three parental inbred lines with classic (BR-13, FT-2 and BR85-29009) and one with long juvenile flowering type (OCEPAR 8) and the F2, F3, and F9 generations derived from all possible crosses between them (including reciprocals) were sowed in September 27th, October 20th and December 17th in 1993 in Londrina, Paraná State, Brazil (between 23o08'47" and 23o55'46" latitude S). The October and December sowing dates are within the period the varietal research personnel recommend for sowing soybeans in Paraná State. The analysis of variance indicated significant differences among sowing dates, among advanced inbred lines, and the sowing date x inbred line interaction. Seed oil content increased from September to October and decreased from October to December in all materials, but the reduction was greater in FT-2 and OCEPAR 8 among the parentals. The additive genetic variance (D) or additive variance among linked genes (D1) was significant for all crosses and sowing dates. Genotype x micro-environment interactions were important in some crosses. The additive [d] effects were greater in September and October, and the additive x additive interaction [i] was important in October among the mean genetic parameters. Significant dominance effects [h] were more frequent in December and October, often in direction of the increased seed oil content. The heritability estimates ranged from 15 to 43%, with the highest values obtained in September. The prediction of cross potential to generate higher seed oil inbred lines indicated that selection is likely to be successful in most crosses. The highest proportion of inbred lines with seed oil percentage above the standard (lines with more than 22% seed oil content) was for BR85-29009 x OCEPAR 8 in September, FT-2 x OCEPAR 8 in October, and in BR85-29009 x OCEPAR 8 and BR-13 x OCEPAR 8 in December.

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Co-location of seed oil content, seed hull content and seed coat color QTL in three different environments in Brassica napus L.

Euphytica ◽

10.1007/s10681-009-0006-5 ◽

2009 ◽

Vol 170 (3) ◽

pp. 355-364 ◽

Cited By ~ 36

Author(s):

X. Y. Yan ◽

J. N. Li ◽

F. Y. Fu ◽

M. Y. Jin ◽

L. Chen ◽

...

Keyword(s):

Brassica Napus ◽

Seed Coat ◽

Oil Content ◽

Seed Oil ◽

Coat Color ◽

Seed Coat Color ◽

Seed Oil Content ◽

Brassica Napus L ◽

Hull Content ◽

Seed Hull

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Investigation of the contribution of oil biosynthetic enzymes to seed oil content in Brassica napus and Arabidopsis thaliana

Canadian Journal of Plant Science ◽

10.4141/cjps2013-161 ◽

2014 ◽

Vol 94 (6) ◽

pp. 1109-1112 ◽

Cited By ~ 3

Author(s):

Vesna Katavic ◽

Lin Shi ◽

Yuanyuan Yu ◽

Lifang Zhao ◽

George W. Haughn ◽

...

Keyword(s):

Arabidopsis Thaliana ◽

Brassica Napus ◽

Seed Coat ◽

Double Haploid ◽

Oil Content ◽

Seed Oil ◽

Fatty Acyl ◽

Seed Oil Content ◽

Oil Biosynthesis ◽

Tag Biosynthesis

Katavic, V., Shi, L., Yu, Y., Zhao, L., Haughn, G. W. and Kunst, L. 2014. Investigation of the contribution of oil biosynthetic enzymes to seed oil content in Brassica napus and Arabidopsis thaliana. Can. J. Plant Sci. 94: 1109–1112. One of the critical reactions in triacylglycerol (TAG) biosynthesis is activation of fatty acyl chains to fatty acyl CoAs, catalyzed by long-chain acyl CoA synthetases (LACS). In Arabidopsis thaliana there is a family of nine genes that encode LACSs. Studies to determine whether the products of two of these genes, LACS8 and LACS9, function together to contribute acyl-CoAs for storage oil biosynthesis in A. thaliana resulted in discovery that it is not LACS8 but LACS1 that functionally overlaps with LACS9 in TAG biosynthesis (published in Plant Journal). To elucidate regulatory mechanisms of seed oil synthesis, the potential roles of phospholipase D zeta (PLDZ) and rhamnose synthase 2 (RHM2/MUM4) in transcription factor GLABRA2 (GL2)-mediated regulation of seed oil biosynthesis and deposition were investigated. Results demonstrated that PLDZ genes are not involved in GL2-mediated seed oil accumulation and that GL2 regulates seed oil production, at least in part, through its influence on expression of the gene RHM2/MUM4 required for the seed coat mucilage biosynthesis (published in Plant Journal). A novel Arabidopsis mutant with speckled seed coat and reduced seed oil phenotypes resulting from a mutation in a single unknown gene was identified, but attempts to isolate the gene by positional cloning have not been successful to date (unpublished results). Finally, seed oil content in near-isogenic double haploid Brassica napus lines was analyzed, “low oil” and “high oil” lines were identified, and developing seeds for expression profiling of target seed oil biosynthesis/bioassembly genes in selected double haploid lines were collected (unpublished results).

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Diagnosis and recommendation integrated system (DRIS) of soybean seed oil content

Revista Brasileira de Ciência do Solo ◽

10.1590/s0100-06832012000600016 ◽

2012 ◽

Vol 36 (6) ◽

pp. 1820-1827 ◽

Cited By ~ 3

Author(s):

Alfredo Castamann ◽

Pedro Alexandre Varella Escosteguy ◽

Diego Berres ◽

Silas Zanella

Keyword(s):

Oil Content ◽

Seed Oil ◽

Soybean Seed ◽

Nutrient Status ◽

Integrated System ◽

Nutrient Ratios ◽

Seed Oil Content ◽

Leaf Analysis ◽

Full Flowering ◽

Soybean Leaves

The Diagnosis and Recommendation Integrated System (DRIS) can improve interpretations of leaf analysis to determine the nutrient status. Diagnoses by this method require DRIS norms, which are however not known for oil content of soybean seeds. The aims of this study were to establish and test the DRIS method for oil content of soybean seed (maturity group II cultivars). Soybean leaves (207 samples) in the full flowering stage were analyzed for macro and micro-nutrients, and the DRIS was applied to assess the relationship between nutrient ratios and the seed oil content. Samples from experimental and farm field sites of the southernmost Brazilian state Rio Grande do Sul (28° - 29° southern latitude; 52° -53° western longitude) were assessed in two growing seasons (2007/2008 and 2008/2009). The DRIS norms related to seed oil content differed between the studied years. A unique DRIS norm was established for seed oil content higher than 18.68 % based on data of the 2007/2008 growing season. Higher DRIS indices of B, Ca, Mg and S were associated with a higher oil content, while the opposite was found for K, N and P. The DRIS can be used to evaluate the leaf nutrient status of soybean to improve the seed oil content of the crop.

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