Single-cell genomics and gene editing: implications for nephrology

2018 ◽  
Vol 15 (2) ◽  
pp. 63-64 ◽  
Author(s):  
Parker C. Wilson ◽  
Benjamin D. Humphreys
Keyword(s):  
Single Cell ◽  
Gene Editing ◽  
Single Cell Genomics

scholarly journals Mapping human cell phenotypes to genotypes with single-cell genomics

Science ◽  
2019 ◽  
Vol 365 (6460) ◽  
pp. 1401-1405 ◽  
Author(s):  
J. Gray Camp ◽  
Randall Platt ◽  
Barbara Treutlein
Keyword(s):  
Single Cell ◽  
Human Cell ◽  
Cell Biology ◽  
Life Histories ◽  
Gene Editing ◽  
Cell Types ◽  
Single Cell Genomics ◽  
Cumulative Activity ◽  
Cellular Phenotypes ◽  
Cell Phenotypes

The cumulative activity of all of the body’s cells, with their myriad interactions, life histories, and environmental experiences, gives rise to a condition that is distinctly human and specific to each individual. It is an enduring goal to catalog our human cell types, to understand how they develop, how they vary between individuals, and how they fail in disease. Single-cell genomics has revolutionized this endeavor because sequencing-based methods provide a means to quantitatively annotate cell states on the basis of high-information content and high-throughput measurements. Together with advances in stem cell biology and gene editing, we are in the midst of a fascinating journey to understand the cellular phenotypes that compose human bodies and how the human genome is used to build and maintain each cell. Here, we will review recent advances into how single-cell genomics is being used to develop personalized phenotyping strategies that cross subcellular, cellular, and tissue scales to link our genome to our cumulative cellular phenotypes.

scholarly journals Gezielte Zellsortierung in der Einzelzellgenomik

BIOspektrum ◽  
2021 ◽  
Vol 27 (3) ◽  
pp. 274-276
Author(s):  
Morgan S. Sobol ◽  
Anne-Kristin Kaster
Keyword(s):  
Dark Matter ◽  
Single Cell ◽  
Cell Sorting ◽  
Genetic Information ◽  
Fluorescent Labeling ◽  
Single Cell Genomics ◽  
Rare Biosphere ◽  
Prokaryotic Genomes ◽  
Selection Of

AbstractSingle cell genomics (SCG) can provide reliable context for assembled genome fragments on the level of individual prokaryotic genomes and has rapidly emerged as an essential complement to cultivation-based and metagenomics research approaches. Targeted cell sorting approaches, which enable the selection of specific taxa by fluorescent labeling, compatible with subsequent single cell genomics offers an opportunity to access genetic information from rare biosphere members which would have otherwise stayed hidden as microbial dark matter.

scholarly journals What's New Is Old: Resolving the Identity of Leptothrix ochracea Using Single Cell Genomics, Pyrosequencing and FISH

PLoS ONE ◽  
2011 ◽  
Vol 6 (3) ◽  
pp. e17769 ◽  
Author(s):  
Emily J. Fleming ◽  
Amy E. Langdon ◽  
Manuel Martinez-Garcia ◽  
Ramunas Stepanauskas ◽  
Nicole J. Poulton ◽  
...  
Keyword(s):  
Single Cell ◽  
Single Cell Genomics

Development of a platform for single cell genomics using convex lens-induced confinement

Lab on a Chip ◽  
2015 ◽  
Vol 15 (14) ◽  
pp. 3013-3020 ◽  
Author(s):  
Sara Mahshid ◽  
Mohammed Jalal Ahamed ◽  
Daniel Berard ◽  
Susan Amin ◽  
Robert Sladek ◽  
...  
Keyword(s):  
Single Cell ◽  
Base Pair ◽  
Single Cell Analysis ◽  
Lab On A Chip ◽  
Cell Analysis ◽  
Next Generation ◽  
Single Cell Genomics ◽  
Convex Lens ◽  
Genomic Dnas

We present a lab-on-a-chip for the next generation of single-cell genomics, performing full-cycle single-cell analysis by demonstrating mega-base pair genomic DNAs in nanochannels extracted in situ.

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