scholarly journals Valorization of sugarcane bagasse by chemical pretreatment and enzyme mediated deconstruction

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Vihang S. Thite ◽  
Anuradha S. Nerurkar
Keyword(s):  
Cell Wall ◽  
Sugarcane Bagasse ◽  
Structural Changes ◽  
Enzymatic Saccharification ◽  
Dry Weight ◽  
Gravimetric Analysis ◽  
Chemical Pretreatment ◽  
Cell Wall Degrading Enzymes ◽  
First Time ◽  
Soluble Components

Abstract After chemical pretreatment, improved amenability of agrowaste biomass for enzymatic saccharification needs an understanding of the effect exerted by pretreatments on biomass for enzymatic deconstruction. In present studies, NaOH, NH4OH and H2SO4 pretreatments effectively changed visible morphology imparting distinct fibrous appearance to sugarcane bagasse (SCB). Filtrate analysis after NaOH, NH4OH and H2SO4 pretreatments yielded release of soluble reducing sugars (SRS) in range of ~0.17–0.44%, ~0.38–0.75% and ~2.9–8.4% respectively. Gravimetric analysis of pretreated SCB (PSCB) biomass also revealed dry weight loss in range of ~25.8–44.8%, ~11.1–16.0% and ~28.3–38.0% by the three pretreatments in the same order. Release of soluble components other than SRS, majorly reported to be soluble lignins, were observed highest for NaOH followed by H2SO4 and NH4OH pretreatments. Decrease or absence of peaks attributed to lignin and loosened fibrous appearance of biomass during FTIR and SEM studies respectively further corroborated with our observations of lignin removal. Application of commercial cellulase increased raw SCB saccharification from 1.93% to 38.84%, 25.56% and 9.61% after NaOH, H2SO4 and NH4OH pretreatments. Structural changes brought by cell wall degrading enzymes were first time shown visually confirming the cell wall disintegration under brightfield, darkfield and fluorescence microscopy. The microscopic evidence and saccharification results proved that the chemical treatment valorized the SCB by making it amenable for enzymatic saccharification.

The Biocontrol Mechanism of Trichoderma asperellum Resistance Plant Pathogenic Fungi

2013 ◽  
Vol 726-731 ◽  
pp. 4525-4528
Author(s):  
Ping Yang ◽  
Qian Xu
Keyword(s):  
Cell Wall ◽  
Plant Pathogens ◽  
Control Plant ◽  
Hydrolytic Enzymes ◽  
Pathogenic Fungi ◽  
Dry Weight ◽  
Plant Pathogenic Fungi ◽  
Cell Wall Degrading Enzymes ◽  
Antifungal Metabolites ◽  
Degrading Enzymes

T. asperellum is an important biocontrol fungus owing to their ability to antagonize plant pathogenic fungi. The biocontrol effects of T. asperellum were played by secreting many kinds of hydrolytic enzymes and antibiotics. T. asperellum producing more cell wall degrading enzymes when meeting plant pathogens. Moreover, the growth of the plant pathogens was inhibited by T. asperellum secondary metabolites. The yield of antibiotic 6-PP was 1.32 mg 6-PP/g mycelial dry weight. T. asperellum control plant pathogens through secreting cell wall degrading enzymes and producing antifungal metabolites.

Antifungal Activity of Biflavones from Taxus baccata and Ginkgo biloba

2003 ◽  
Vol 58 (1-2) ◽  
pp. 65-69 ◽  
Author(s):  
Mirosława Krauze-Baranowska ◽  
Marian Wiwart
Keyword(s):  
Cell Wall ◽  
Image Analysis ◽  
Antifungal Activity ◽  
Ginkgo Biloba ◽  
Alternaria Alternata ◽  
Structural Changes ◽  
Fusarium Culmorum ◽  
Taxus Baccata ◽  
Computer Aided ◽  
First Time

Bilobetin and 4‴-O-methylamentoflavone were isolated and identified in the needles of Taxus baccata, for the first time in this species. The antifungal activity of biflavones from T. baccata and Ginkgo biloba, namely amentoflavone, 7-O-methylamentoflavone, bilobetin, ginkgetin, sciadopitysin and 2,3-dihydrosciadopitysin towards the fungi Alternaria alternata, Fusarium culmorum, Cladosporium oxysporum was determined employing computer-aided image analysis couplet to a microscope. Bilobetin exhibited a significant antifungal activity with values of ED50 14, 11 and 17 μm respectively. This compound completely inhibited the growth of germinating tubes of Cladosporium oxysporum and Fusarium culmorum at a concentration 100 μm. Activity of ginkgetin and 7-O-methylamentoflavone towards Alternaria alternata was stronger than that of bilobetin. Moreover, slight structural changes in the cell wall of Alternaria alternata exposed to ginkgetin at concentration of 200 μm were observed

scholarly journals Discovery and Use of Genes and Gene Combinations Coding for Proteins Useful in Biological Control

1994 ◽  
Author(s):  
Gary E. Harman ◽  
Ilan Chet
Keyword(s):  
Cell Wall ◽  
Pathogenic Fungi ◽  
Plant Pathogenic Fungi ◽  
Cell Wall Degrading Enzymes ◽  
Fungal Cell ◽  
Degrading Enzymes ◽  
Transgenic Organisms ◽  
Genetic Sequences ◽  
High Level ◽  
First Time

The objectives of the research in this proposal were to (A) identify synergy among proteins that provide enhanced activity over single proteins for control of plant pathogenic fungi, (B) clone and characterize genetic sequences coding for proteins with ability to control pathogenic fungi, (C) produce transgenic organisms with enhanced biocontrol ability using genes and gene combinations and determine their efficiency in protecting plants against plant pathogenic fungi. A related objective was to produce disease-resistant plants. Fungal cell wall degrading enzymes from any source are strongly synergistic with any membrane active compound and, further, different classes of cell wall degrading enzymes are also strongly synergistic. We have cloned and sequenced a number of genes from bacterial and fungal sources including five that are structurally unrelated. We have prepared transgenic fungi that are deficient in production of enzymes and useful in mechanistic studies. Others are hyperproducers of specific enzymes that permit us, for the first time, to produce enzymes from T. harzianum in sufficient quantity to conduct tests of their potential use in commercial agriculture. Finally, genes from these studies have been inserted into several species of crop plants were they produce a high level of resistance to several plant pathogenic fungi.

Alkaliphilic species Euastrum lacustre (Charophyta, Zygnematophyceae), first record from Russia

2020 ◽  
Vol 54 (2) ◽  
pp. 313-320
Author(s):  
O. V. Anissimova
Keyword(s):  
Cell Wall ◽  
New Species ◽  
First Record ◽  
European Russia ◽  
Similar Species ◽  
Morphological Differences ◽  
First Time ◽  
Kursk Region

Euastrum lacustre is reported for Russia for the first time. This alcaliphilic species was found in the periphyton and plankton of three lakes in the Kursk Region (European Russia). A description of morphology, including the relief of cell wall, and habitats where this taxon is found are represented. LM and SEM microphotographs are provided. Morphological differences of E. lacustre from similar species are discussed. New species for region, namely Closterium aciculare, Cosmarium formosulum, C. granatum, C. pseudoinsigne, C. reniforme and Staurastrum pingue, are found in the samples together with E. lacustre.

scholarly journals Pressurized Hot Liquid Extraction with 15% v/v Glycerol-Water as an Effective Environment-Friendly Process to Obtain Durvillaea Incurvata and Lessonia Spicata Phlorotannin Extracts with Antioxidant and Antihyperglycemic Potential

Antioxidants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1105
Author(s):  
Fernanda Erpel ◽  
María Salomé Mariotti-Celis ◽  
Javier Parada ◽  
Franco Pedreschi ◽  
José Ricardo Pérez-Correa
Keyword(s):  
Brown Seaweed ◽  
Dry Weight ◽  
Liquid Extraction ◽  
Southern Chile ◽  
Polyphenol Content ◽  
Glycerol Water ◽  
Environment Friendly ◽  
Dry Extract ◽  
First Time ◽  
Anatomical Part

Brown seaweed phlorotannins have shown the potential to promote several health benefits. Durvillaea incurvata and Lessonia spicata—species that are widely distributed in central and southern Chile—were investigated to obtain phlorotannin extracts with antioxidant and antihyperglycemic potential. The use of an environmentally friendly and food-grade glycerol-based pressurized hot liquid extraction (PHLE) process (15% v/v glycerol water) was assessed for the first time to obtain phlorotannins. Multiple effects were analyzed, including the effect of the species, harvesting area (Las Cruces and Niebla), and anatomical part (holdfast, stipe, and frond) on the extracts’ polyphenol content (TPC), antioxidant capacity (AC), and carbohydrate-hydrolyzing enzyme—α-glucosidase and α-amylase—inhibitory activity. Contaminants, such as mannitol, heavy metals (As, Cd, Pb, Hg, and Sn), and 5-hydroxymethylfurfural (HMF), were also determined. The anatomical part used demonstrated a significant impact on the extracts’ TPC and AC, with holdfasts showing the highest values (TPC: 95 ± 24 mg phloroglucinol equivalents/g dry extract; DPPH: 400 ± 140 μmol Trolox equivalents/g dry extract; ORAC: 560 ± 130 μmol TE/g dry extract). Accordingly, holdfast extracts presented the most potent α-glucosidase inhibition, with D. incurvata from Niebla showing an activity equivalent to fifteen times that of acarbose. Only one frond and stipe extract showed significant α-glucosidase inhibitory capacity. No α-amylase inhibition was found in any extract. Although no HMF was detected, potentially hazardous cadmium levels (over the French limit) and substantial mannitol concentrations—reaching up to 50% of the extract dry weight—were found in most seaweed samples and extracts. Therefore, further purification steps are suggested if food or pharmaceutical applications are intended for the seaweed PHLE extracts obtained in this study.

scholarly journals Alkali-Hydrothermal Treatment of K-Rich Igneous Rocks for Their Direct Use as Potassic Fertilizers

Minerals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 140
Author(s):  
Aaron Mbissik ◽  
Abdellatif Elghali ◽  
Muhammad Ouabid ◽  
Otmane Raji ◽  
Jean-Louis Bodinier ◽  
...  
Keyword(s):  
Hydrothermal Treatment ◽  
Inductively Coupled Plasma ◽  
Structural Changes ◽  
Raw Materials ◽  
Hydrothermal Process ◽  
Atomic Emission ◽  
Igneous Rocks ◽  
Gravimetric Analysis ◽  
African Countries ◽  
Naoh Solution

Due to the increasing demand for conventional sources of potassium (K) and their inaccessibility by African countries, K-rich igneous rocks are increasingly studied as potential alternative sources. In this study, six potassic igneous rocks (syenites and trachytes) from the Tamazeght, Jbel Boho, Ait Saoun, and El Glo’a regions (Morocco) were sampled and characterized. Then they were hydrothermally treated to enhance their K release for potential use as potassic fertilizers. The raw materials are mainly formed by microcline (up to 74%), orthoclase (20–68%), albite (36–57%), biotite-muscovite (15–23%), and titanite, calcite, hematite, and apatite as accessory minerals. These samples were crushed and milled to reach a particle size <150 µm and mixed with 4 N NaOH solution in an autoclave. The liquid/solid (L/S) ratio was about 44 mL/50 g. The powders were allowed to react with the solution at 170 °C for 7 h. For all tests, NaOH reacted completely with the powders and no liquid was observed after the treatment. X-ray diffraction (XRD), thermal gravimetric analysis (TGA), infrared spectroscopy (IRTF), and scanning electron microscopy (SEM-EDS) were carried out on treated samples to characterize the mineralogical and structural changes due to the alkali-hydrothermal treatment. Indeed, the treated samples revealed the presence of sodic neoformed phases such as thermonatrite, sodalite, analcime, and cancrinite. The treated material was leached for a week using deionized water and the elements released were measured using inductively coupled plasma–atomic emission spectroscopy (ICP-AES). The hydrothermal process showed a strong effect on structure breakdown as well as on the release of K and other nutrients such as P, Fe, Si, Mg, and Ca. Therefore, the alkali-hydrothermal treatment allowed the release of 50.5 wt% K. Moreover, the release of Mg, Ca, Fe, P, K, and Si were significantly increased. Mg, Ca, Fe, P, K, and Si release within raw materials was about (0.5–3.6), (3.5–31.4), (0.01–0.4), (0.01–0.3), (20–55), and (4.6–8) mg/kg, respectively, whereas treated samples showed a higher release of these elements. Quantitatively, Mg, Ca, Fe, P, K, and Si releases were about (10–11.8), (60–70), (7–20), (1.2–15), (218–1278), and (1119–2759) mg/kg, respectively. Consequently, the treated igneous rocks (syenite and trachyte) could be directly used as potassic fertilizers that would also be a source of other nutrients.

Cell Wall Permeability of Pinto Bean Cotyledon Cells Regulates In Vitro Fecal Fermentation and Gut Microbiota

2021 ◽  
Author(s):  
Yanrong Huang ◽  
Sushil Dhital ◽  
Feitong Liu ◽  
Xiong Fu ◽  
Qiang Huang ◽  
...  
Keyword(s):  
Cell Wall ◽  
Structural Changes ◽  
Microbiota Composition ◽  
Colonic Fermentation ◽  
Pinto Bean ◽  
Wall Permeability ◽  
Cell Wall Permeability ◽  
Cotyledon Cells ◽  
Whole Foods

Processing induced structural changes of whole foods on regulation of colonic fermentation rate and microbiota composition are least understood and often overlooked. In the present study, intact cotyledon cells from...

scholarly journals Antioxidant Capacity and Phenolic and Sugar Profiles of Date Fruits Extracts from Six Different Algerian Cultivars as Influenced by Ripening Stages and Extraction Systems

Foods ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 503
Author(s):  
Malika Tassoult ◽  
Djamel Edine Kati ◽  
María África Fernández-Prior ◽  
Alejandra Bermúdez-Oria ◽  
Juan Fernandez-Bolanos ◽  
...  
Keyword(s):  
Sugar Content ◽  
Antioxidant Properties ◽  
Dry Weight ◽  
Natural Antioxidants ◽  
Antioxidant Power ◽  
Fruit Extracts ◽  
Ripening Stages ◽  
Date Fruit ◽  
Ferric Reducing Antioxidant Power ◽  
First Time

The study investigated the phenols, sugar and the antioxidant capacities of date fruit extracts obtained by organic solvents and by hydrothermal treatment from six different Algerian cultivars at two ripening stages for the first time. The analyzed cultivars exhibited potent antioxidant properties (ferric reducing antioxidant power (FRAP), 1,1-Diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) scavenging capacities) and different phenols regardless of the solvents and the maturity stages. About 18 phenols were identified and quantified, mainly in the hydrothermal extracts. The earlier stages were characterized by high amounts of o-coumaric acid, cinnamic acid and luteolin, with a noticeable absence of quercetin. The tamr stage presented the highest sugar content (78.15–86.85 mg/100 mg dry weight (DW)) with an abundance of glucose. Galactose was present only in some cultivars from the kimri stage (tamjouhert). Uronic acids were mostly detected at the tamr stage (4.02–8.82 mg gallic acid equivalent/100 mg dried weight). The obtained results highlight the potential of using date fruit extracts as natural antioxidants, especially at industrial scales that tend use hydrothermal extraction.

scholarly journals Overexpression of vesicle-associated membrane protein PttVAP27-17 as a tool to improve biomass production and the overall saccharification yields in Populus trees

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Madhavi Latha Gandla ◽  
Niklas Mähler ◽  
Sacha Escamez ◽  
Tomas Skotare ◽  
Ogonna Obudulu ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Biomass Production ◽  
Enzymatic Saccharification ◽  
Dry Weight ◽  
Populus Tremula ◽  
Transgenic Trees ◽  
Wild Type ◽  
Glucose Yield ◽  
Transgenic Lines ◽  
Wild Type Control

Abstract Background Bioconversion of wood into bioproducts and biofuels is hindered by the recalcitrance of woody raw material to bioprocesses such as enzymatic saccharification. Targeted modification of the chemical composition of the feedstock can improve saccharification but this gain is often abrogated by concomitant reduction in tree growth. Results In this study, we report on transgenic hybrid aspen (Populus tremula × tremuloides) lines that showed potential to increase biomass production both in the greenhouse and after 5 years of growth in the field. The transgenic lines carried an overexpression construct for Populus tremula × tremuloides vesicle-associated membrane protein (VAMP)-associated protein PttVAP27-17 that was selected from a gene-mining program for novel regulators of wood formation. Analytical-scale enzymatic saccharification without any pretreatment revealed for all greenhouse-grown transgenic lines, compared to the wild type, a 20–44% increase in the glucose yield per dry weight after enzymatic saccharification, even though it was statistically significant only for one line. The glucose yield after enzymatic saccharification with a prior hydrothermal pretreatment step with sulfuric acid was not increased in the greenhouse-grown transgenic trees on a dry-weight basis, but increased by 26–50% when calculated on a whole biomass basis in comparison to the wild-type control. Tendencies to increased glucose yields by up to 24% were present on a whole tree biomass basis after acidic pretreatment and enzymatic saccharification also in the transgenic trees grown for 5 years on the field when compared to the wild-type control. Conclusions The results demonstrate the usefulness of gene-mining programs to identify novel genes with the potential to improve biofuel production in tree biotechnology programs. Furthermore, multi-omic analyses, including transcriptomic, proteomic and metabolomic analyses, performed here provide a toolbox for future studies on the function of VAP27 proteins in plants.

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